Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers
Human milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers’ body mass index, there is limited information on the possible influence of this condition on th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/4/887 |
| _version_ | 1797537236648984576 |
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| author | Silvia Sánchez-Hernández Laëtitia Théron Pablo Jiménez-Barrios Manuel Olalla-Herrera Isidra Recio Beatriz Miralles |
| author_facet | Silvia Sánchez-Hernández Laëtitia Théron Pablo Jiménez-Barrios Manuel Olalla-Herrera Isidra Recio Beatriz Miralles |
| author_sort | Silvia Sánchez-Hernández |
| collection | DOAJ |
| description | Human milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers’ body mass index, there is limited information on the possible influence of this condition on the whole protein distribution. The objective of this study was to evaluate the protein profile of human milk from normal weight and overweight or obese mothers to identify differences in protein expression in colostrum, transitional and mature milk. The mass spectrometry analysis showed the ability to class with a high degree of confidence the lactation state and the milk profile according to the mother’s condition. Individual milk samples were subjected to a digestion in vitro model that takes into account the specificities of the gastrointestinal conditions of full-term newborn infants. The digestion products were compared with available data from the digestive contents in newborns. The behavior of the most abundant proteins and the overall peptide generation and survival, showed good correspondence with in vivo data. |
| first_indexed | 2024-03-10T12:12:15Z |
| format | Article |
| id | doaj.art-1d2a48e1111f4b5ba149617a7f025358 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T12:12:15Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-1d2a48e1111f4b5ba149617a7f0253582023-11-21T16:06:25ZengMDPI AGFoods2304-81582021-04-0110488710.3390/foods10040887Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight MothersSilvia Sánchez-Hernández0Laëtitia Théron1Pablo Jiménez-Barrios2Manuel Olalla-Herrera3Isidra Recio4Beatriz Miralles5Departamento de Nutrición y Bromatología, University of Granada, 18071 Granada, SpainInstitut National de Recherche Pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), UR370 Qualité des Produits Animaux, F-63122 Saint Genès-Champanelle, FranceInstituto de Investigación en Ciencias de la Alimentación, CIAL, (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, SpainDepartamento de Nutrición y Bromatología, University of Granada, 18071 Granada, SpainInstituto de Investigación en Ciencias de la Alimentación, CIAL, (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, SpainInstituto de Investigación en Ciencias de la Alimentación, CIAL, (CSIC-UAM), Nicolás Cabrera 9, 28049 Madrid, SpainHuman milk proteins have shown to vary in concentration and distribution through lactation. However, while some regulatory components, such as hormones, have shown associations with regard to the mothers’ body mass index, there is limited information on the possible influence of this condition on the whole protein distribution. The objective of this study was to evaluate the protein profile of human milk from normal weight and overweight or obese mothers to identify differences in protein expression in colostrum, transitional and mature milk. The mass spectrometry analysis showed the ability to class with a high degree of confidence the lactation state and the milk profile according to the mother’s condition. Individual milk samples were subjected to a digestion in vitro model that takes into account the specificities of the gastrointestinal conditions of full-term newborn infants. The digestion products were compared with available data from the digestive contents in newborns. The behavior of the most abundant proteins and the overall peptide generation and survival, showed good correspondence with in vivo data.https://www.mdpi.com/2304-8158/10/4/887human milkMALDI mass spectrometrybody mass indexin vitro infant digestionbioactive proteins |
| spellingShingle | Silvia Sánchez-Hernández Laëtitia Théron Pablo Jiménez-Barrios Manuel Olalla-Herrera Isidra Recio Beatriz Miralles Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers Foods human milk MALDI mass spectrometry body mass index in vitro infant digestion bioactive proteins |
| title | Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers |
| title_full | Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers |
| title_fullStr | Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers |
| title_full_unstemmed | Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers |
| title_short | Protein Profile and Simulated Digestive Behavior of Breast Milk from Overweight and Normal Weight Mothers |
| title_sort | protein profile and simulated digestive behavior of breast milk from overweight and normal weight mothers |
| topic | human milk MALDI mass spectrometry body mass index in vitro infant digestion bioactive proteins |
| url | https://www.mdpi.com/2304-8158/10/4/887 |
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