Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea
Abstract Background Enterotoxigenic Escherichia coli (ETEC) cause infectious diarrhea and diarrheal death. However, the genetic properties of pathogenic strains vary spatially and temporally, making prevention and treatment difficult. In this study, the genomic features of the major type of ETEC in...
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| Format: | Article |
| Language: | English |
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BMC
2017-09-01
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| Series: | Gut Pathogens |
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| Online Access: | http://link.springer.com/article/10.1186/s13099-017-0204-y |
| _version_ | 1818112817047273472 |
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| author | Taesoo Kwon Si-yun Chung Young-Hee Jung Su-Jin Jung Sang-Gyun Roh Je-Seop Park Cheorl-Ho Kim Won Kim Young-Seok Bak Seung-Hak Cho |
| author_facet | Taesoo Kwon Si-yun Chung Young-Hee Jung Su-Jin Jung Sang-Gyun Roh Je-Seop Park Cheorl-Ho Kim Won Kim Young-Seok Bak Seung-Hak Cho |
| author_sort | Taesoo Kwon |
| collection | DOAJ |
| description | Abstract Background Enterotoxigenic Escherichia coli (ETEC) cause infectious diarrhea and diarrheal death. However, the genetic properties of pathogenic strains vary spatially and temporally, making prevention and treatment difficult. In this study, the genomic features of the major type of ETEC in Korea from 2003 to 2011 were examined by whole-genome sequencing of strain NCCP15740, and a comparative genomic analysis was performed with O6 reference strains. Results The assembled genome size of NCCP15740 was 4,795,873 bp with 50.54% G+C content. Using rapid annotation using subsystem technology analysis, we predicted 4492 ORFs and 17 RNA genes. NCCP15740 was investigated for enterotoxin genes, colonization factor (CF) genes, serotype, multilocus sequence typing (MLST) profiles, and classical and nonclassical virulence factors. NCCP15740 belonged to the O6:H16 serotype and possessed enterotoxin genes encoding heat-stable toxin (STh) and heat-labile toxin (LT); 87.5% of the O6 serotype strains possessed both toxin types. NCCP15740 carried the colonization factors CS2 and CS3, whereas most O6 strains carried CS2-CS3-CS21 (79.2%). NCCP15740 harbored fewer virulence factors (59.4%) than the average observed in other O6 strains (62.0%). Interestingly, NCCP15740 did not harbor any nonclassical virulence genes. Conclusions The major type of ETEC in Korea had the same MLST sequence type as that of isolates from the USA obtained in 2011 and 2014, but had different colonization factor types and virulence profiles. These results provide important information for the development of an ETEC vaccine candidate. |
| first_indexed | 2024-12-11T03:24:57Z |
| format | Article |
| id | doaj.art-1d2a66c0c8d44639b005661f67b5466b |
| institution | Directory Open Access Journal |
| issn | 1757-4749 |
| language | English |
| last_indexed | 2024-12-11T03:24:57Z |
| publishDate | 2017-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Gut Pathogens |
| spelling | doaj.art-1d2a66c0c8d44639b005661f67b5466b2022-12-22T01:22:32ZengBMCGut Pathogens1757-47492017-09-01911710.1186/s13099-017-0204-yComparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in KoreaTaesoo Kwon0Si-yun Chung1Young-Hee Jung2Su-Jin Jung3Sang-Gyun Roh4Je-Seop Park5Cheorl-Ho Kim6Won Kim7Young-Seok Bak8Seung-Hak Cho9Interdisciplinary Program in Bioinformatics, Seoul National UniversityInterdisciplinary Program in Bioinformatics, Seoul National UniversityDepartment of Biotechnology, Korea UniversityDepartment of Dental Hygiene, College of Medical Science, Konyang UniversityDepartment of Emergency Medical Services, Sun Moon UniversityFire Science Laboratory, National Fire Service AcademyGlycobiology Unit, Department of Biological Science, Sungkyunkwan University and Samsung Advanced Institute for Health Sciences and Technology (SAIHST)Interdisciplinary Program in Bioinformatics, Seoul National UniversityDepartment of Emergency Medical Services, Sun Moon UniversityDivision of Enteric Diseases, Center for Infectious Diseases, Korea National Institute of HealthAbstract Background Enterotoxigenic Escherichia coli (ETEC) cause infectious diarrhea and diarrheal death. However, the genetic properties of pathogenic strains vary spatially and temporally, making prevention and treatment difficult. In this study, the genomic features of the major type of ETEC in Korea from 2003 to 2011 were examined by whole-genome sequencing of strain NCCP15740, and a comparative genomic analysis was performed with O6 reference strains. Results The assembled genome size of NCCP15740 was 4,795,873 bp with 50.54% G+C content. Using rapid annotation using subsystem technology analysis, we predicted 4492 ORFs and 17 RNA genes. NCCP15740 was investigated for enterotoxin genes, colonization factor (CF) genes, serotype, multilocus sequence typing (MLST) profiles, and classical and nonclassical virulence factors. NCCP15740 belonged to the O6:H16 serotype and possessed enterotoxin genes encoding heat-stable toxin (STh) and heat-labile toxin (LT); 87.5% of the O6 serotype strains possessed both toxin types. NCCP15740 carried the colonization factors CS2 and CS3, whereas most O6 strains carried CS2-CS3-CS21 (79.2%). NCCP15740 harbored fewer virulence factors (59.4%) than the average observed in other O6 strains (62.0%). Interestingly, NCCP15740 did not harbor any nonclassical virulence genes. Conclusions The major type of ETEC in Korea had the same MLST sequence type as that of isolates from the USA obtained in 2011 and 2014, but had different colonization factor types and virulence profiles. These results provide important information for the development of an ETEC vaccine candidate.http://link.springer.com/article/10.1186/s13099-017-0204-yEnterotoxigenic Escherichia coli O6Whole-genome sequencingColonization factor genesEnterotoxinMultilocus sequence typingVirulence |
| spellingShingle | Taesoo Kwon Si-yun Chung Young-Hee Jung Su-Jin Jung Sang-Gyun Roh Je-Seop Park Cheorl-Ho Kim Won Kim Young-Seok Bak Seung-Hak Cho Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea Gut Pathogens Enterotoxigenic Escherichia coli O6 Whole-genome sequencing Colonization factor genes Enterotoxin Multilocus sequence typing Virulence |
| title | Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea |
| title_full | Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea |
| title_fullStr | Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea |
| title_full_unstemmed | Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea |
| title_short | Comparative genomic analysis and characteristics of NCCP15740, the major type of enterotoxigenic Escherichia coli in Korea |
| title_sort | comparative genomic analysis and characteristics of nccp15740 the major type of enterotoxigenic escherichia coli in korea |
| topic | Enterotoxigenic Escherichia coli O6 Whole-genome sequencing Colonization factor genes Enterotoxin Multilocus sequence typing Virulence |
| url | http://link.springer.com/article/10.1186/s13099-017-0204-y |
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