miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis
Abstract Background Atresia and degeneration, a follicular developmental fate that reduces female fertility and is triggered by granulosa cell (GC) apoptosis, have been induced by dozens of miRNAs. Here, we report a miRNA, miR-423, that inhibits the initiation of follicular atresia (FA), and early a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Journal of Animal Science and Biotechnology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40104-023-00960-y |
| _version_ | 1797397795967074304 |
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| author | Yuqi Li Zhuofan Zhang Siqi Wang Xing Du Qifa Li |
| author_facet | Yuqi Li Zhuofan Zhang Siqi Wang Xing Du Qifa Li |
| author_sort | Yuqi Li |
| collection | DOAJ |
| description | Abstract Background Atresia and degeneration, a follicular developmental fate that reduces female fertility and is triggered by granulosa cell (GC) apoptosis, have been induced by dozens of miRNAs. Here, we report a miRNA, miR-423, that inhibits the initiation of follicular atresia (FA), and early apoptosis of GCs. Results We showed that miR-423 was down-regulated during sow FA, and its levels in follicles were negatively correlated with the GC density and the P4/E2 ratio in the follicular fluid in vivo. The in vitro gain-of-function experiments revealed that miR-423 suppresses cell apoptosis, especially early apoptosis in GCs. Mechanically speaking, the miR-423 targets and interacts with the 3'-UTR of the porcine SMAD7 gene, which encodes an apoptosis-inducing factor in GCs, and represses its expression and pro-apoptotic function. Interestingly, FA and the GC apoptosis-related lncRNA NORHA was demonstrated as a ceRNA of miR-423. Additionally, we showed that a single base deletion/insertion in the miR-423 promoter is significantly associated with the number of stillbirths (NSB) trait of sows. Conclusion These results demonstrate that miR-423 is a small molecule for inhibiting FA initiation and GC early apoptosis, suggesting that treating with miR-423 may be a novel approach for inhibiting FA initiation and improving female fertility. |
| first_indexed | 2024-03-09T01:16:18Z |
| format | Article |
| id | doaj.art-1d2b2ccb68834a58be757988038056cc |
| institution | Directory Open Access Journal |
| issn | 2049-1891 |
| language | English |
| last_indexed | 2024-03-09T01:16:18Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Animal Science and Biotechnology |
| spelling | doaj.art-1d2b2ccb68834a58be757988038056cc2023-12-10T12:27:23ZengBMCJournal of Animal Science and Biotechnology2049-18912023-12-0114111310.1186/s40104-023-00960-ymiR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosisYuqi Li0Zhuofan Zhang1Siqi Wang2Xing Du3Qifa Li4College of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityCollege of Animal Science and Technology, Nanjing Agricultural UniversityAbstract Background Atresia and degeneration, a follicular developmental fate that reduces female fertility and is triggered by granulosa cell (GC) apoptosis, have been induced by dozens of miRNAs. Here, we report a miRNA, miR-423, that inhibits the initiation of follicular atresia (FA), and early apoptosis of GCs. Results We showed that miR-423 was down-regulated during sow FA, and its levels in follicles were negatively correlated with the GC density and the P4/E2 ratio in the follicular fluid in vivo. The in vitro gain-of-function experiments revealed that miR-423 suppresses cell apoptosis, especially early apoptosis in GCs. Mechanically speaking, the miR-423 targets and interacts with the 3'-UTR of the porcine SMAD7 gene, which encodes an apoptosis-inducing factor in GCs, and represses its expression and pro-apoptotic function. Interestingly, FA and the GC apoptosis-related lncRNA NORHA was demonstrated as a ceRNA of miR-423. Additionally, we showed that a single base deletion/insertion in the miR-423 promoter is significantly associated with the number of stillbirths (NSB) trait of sows. Conclusion These results demonstrate that miR-423 is a small molecule for inhibiting FA initiation and GC early apoptosis, suggesting that treating with miR-423 may be a novel approach for inhibiting FA initiation and improving female fertility.https://doi.org/10.1186/s40104-023-00960-yGranulosa cell apoptosismiR-423NORHASMAD7Sow fertility traits |
| spellingShingle | Yuqi Li Zhuofan Zhang Siqi Wang Xing Du Qifa Li miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis Journal of Animal Science and Biotechnology Granulosa cell apoptosis miR-423 NORHA SMAD7 Sow fertility traits |
| title | miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis |
| title_full | miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis |
| title_fullStr | miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis |
| title_full_unstemmed | miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis |
| title_short | miR-423 sponged by lncRNA NORHA inhibits granulosa cell apoptosis |
| title_sort | mir 423 sponged by lncrna norha inhibits granulosa cell apoptosis |
| topic | Granulosa cell apoptosis miR-423 NORHA SMAD7 Sow fertility traits |
| url | https://doi.org/10.1186/s40104-023-00960-y |
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