A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approaches
Mouse bone marrow mesenchymal stem cells (mBM‐MSCs) are important for preclinical tissue regeneration and repair studies. In the present study, we isolated mBM‐MSCs using three easy‐to‐perform methods (whole bone marrow‐adherent culture, density‐gradient centrifugation, and bone digestion), and then...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | FEBS Open Bio |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/2211-5463.13493 |
| _version_ | 1798014673744822272 |
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| author | Yalan Lu Yunlin Han Li Zhou Guiying Shi Lin Bai Kewei Wang Chuan Qin |
| author_facet | Yalan Lu Yunlin Han Li Zhou Guiying Shi Lin Bai Kewei Wang Chuan Qin |
| author_sort | Yalan Lu |
| collection | DOAJ |
| description | Mouse bone marrow mesenchymal stem cells (mBM‐MSCs) are important for preclinical tissue regeneration and repair studies. In the present study, we isolated mBM‐MSCs using three easy‐to‐perform methods (whole bone marrow‐adherent culture, density‐gradient centrifugation, and bone digestion), and then compared the morphology, proliferation, differentiation, and paracrine factor profiles of the isolated mBM‐MSCs. Of these three isolation methods, the bone digestion method resulted in the highest quantity of mBM‐MSCs with high growth potential and moderate differentiation. Conversely, the mBM‐MSCs isolated through the whole bone marrow‐adherent method exhibited the lowest potency for proliferation and differentiation. The differentially expressed factors between mBM‐MSCs were primarily those involved in immune responses. The highly expressed secreted factors included cytokines/members of the chemokine family, growth factors, and protein binding/proteinase activity. These findings provide a fundamental reference for development of MSC isolation methods. |
| first_indexed | 2024-04-11T15:22:13Z |
| format | Article |
| id | doaj.art-1d2e564807e94e2b802b3ac35016ef01 |
| institution | Directory Open Access Journal |
| issn | 2211-5463 |
| language | English |
| last_indexed | 2024-04-11T15:22:13Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | FEBS Open Bio |
| spelling | doaj.art-1d2e564807e94e2b802b3ac35016ef012022-12-22T04:16:21ZengWileyFEBS Open Bio2211-54632022-12-0112122154216510.1002/2211-5463.13493A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approachesYalan Lu0Yunlin Han1Li Zhou2Guiying Shi3Lin Bai4Kewei Wang5Chuan Qin6Beijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China, Institute of Laboratory Animal Science, Peking Union Medicine College Chinese Academy of Medical Sciences Beijing ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China, Institute of Laboratory Animal Science, Peking Union Medicine College Chinese Academy of Medical Sciences Beijing ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China, Institute of Laboratory Animal Science, Peking Union Medicine College Chinese Academy of Medical Sciences Beijing ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China, Institute of Laboratory Animal Science, Peking Union Medicine College Chinese Academy of Medical Sciences Beijing ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China, Institute of Laboratory Animal Science, Peking Union Medicine College Chinese Academy of Medical Sciences Beijing ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China, Institute of Laboratory Animal Science, Peking Union Medicine College Chinese Academy of Medical Sciences Beijing ChinaBeijing Engineering Research Center for Experimental Animal Models of Human Critical Diseases, Key Laboratory of Human Disease Comparative Medicine, National Health Commission of China, Institute of Laboratory Animal Science, Peking Union Medicine College Chinese Academy of Medical Sciences Beijing ChinaMouse bone marrow mesenchymal stem cells (mBM‐MSCs) are important for preclinical tissue regeneration and repair studies. In the present study, we isolated mBM‐MSCs using three easy‐to‐perform methods (whole bone marrow‐adherent culture, density‐gradient centrifugation, and bone digestion), and then compared the morphology, proliferation, differentiation, and paracrine factor profiles of the isolated mBM‐MSCs. Of these three isolation methods, the bone digestion method resulted in the highest quantity of mBM‐MSCs with high growth potential and moderate differentiation. Conversely, the mBM‐MSCs isolated through the whole bone marrow‐adherent method exhibited the lowest potency for proliferation and differentiation. The differentially expressed factors between mBM‐MSCs were primarily those involved in immune responses. The highly expressed secreted factors included cytokines/members of the chemokine family, growth factors, and protein binding/proteinase activity. These findings provide a fundamental reference for development of MSC isolation methods.https://doi.org/10.1002/2211-5463.13493bone digestiondensity‐gradient centrifugationmouse bone marrow mesenchymal stem cellssecretomewhole bone marrow‐adherent culture |
| spellingShingle | Yalan Lu Yunlin Han Li Zhou Guiying Shi Lin Bai Kewei Wang Chuan Qin A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approaches FEBS Open Bio bone digestion density‐gradient centrifugation mouse bone marrow mesenchymal stem cells secretome whole bone marrow‐adherent culture |
| title | A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approaches |
| title_full | A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approaches |
| title_fullStr | A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approaches |
| title_full_unstemmed | A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approaches |
| title_short | A comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy‐to‐perform approaches |
| title_sort | comparative study of mouse bone marrow mesenchymal stem cells isolated using three easy to perform approaches |
| topic | bone digestion density‐gradient centrifugation mouse bone marrow mesenchymal stem cells secretome whole bone marrow‐adherent culture |
| url | https://doi.org/10.1002/2211-5463.13493 |
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