Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase
The biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soils by laccase is reported. However, the low laccase activities of free laccase have limited its applications in environmental bioremediation. In this study, polluted soil was made with 3:1 volume ratio of soil to PAH solution. Subse...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Serbian Chemical Society
2018-01-01
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| Series: | Journal of the Serbian Chemical Society |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0352-5139/2018/0352-51391800022W.pdf |
| _version_ | 1811277177401901056 |
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| author | Wang Xin Shi Yu Sun Ni Zi Jun Li Zhao Xing Bao Jia |
| author_facet | Wang Xin Shi Yu Sun Ni Zi Jun Li Zhao Xing Bao Jia |
| author_sort | Wang Xin |
| collection | DOAJ |
| description | The biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soils by laccase is reported. However, the low laccase activities of free laccase have limited its applications in environmental bioremediation. In this study, polluted soil was made with 3:1 volume ratio of soil to PAH solution. Subsequently, the adsorption–cross-link composite immobilization method was applied to immobilize laccase derived from fungi onto nylon net and chitosan, respectively. These two kinds of immobilized laccase were used in the degradation of pyrene (Pyr) and benzo[a]pyrene (BaP), and their degradation efficiencies under different temperature and pH conditions were investigated. Consequently, the optimal laboratory experimental parameters were determined as follows: first, compared with free laccase, the degradation rates of Pyr and BaP by immobilized laccase increased by around 10–30 %; second, the degradation efficiency of chitosan as a carrier of immobilized laccase was much better than that of nylon net as a carrier of the immobilized laccase; finally, when the temperature was set at 40°C and the pH was set at 4, the degradation efficiency achieved by immobilized laccase was the best. |
| first_indexed | 2024-04-13T00:11:05Z |
| format | Article |
| id | doaj.art-1d3ad50f578b49fab9373a424f9c1124 |
| institution | Directory Open Access Journal |
| issn | 0352-5139 1820-7421 |
| language | English |
| last_indexed | 2024-04-13T00:11:05Z |
| publishDate | 2018-01-01 |
| publisher | Serbian Chemical Society |
| record_format | Article |
| series | Journal of the Serbian Chemical Society |
| spelling | doaj.art-1d3ad50f578b49fab9373a424f9c11242022-12-22T03:11:05ZengSerbian Chemical SocietyJournal of the Serbian Chemical Society0352-51391820-74212018-01-0183554955910.2298/JSC171004022W0352-51391800022WDegradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccaseWang Xin0Shi Yu Sun1Ni Zi Jun2Li Zhao Xing3Bao Jia4Shenyang University of Technology, School of Science, Shenyang, ChinaShenyang University of Technology, School of Science, Shenyang, ChinaShenyang University of Technology, School of Science, Shenyang, ChinaShenyang University of Technology, School of Science, Shenyang, ChinaShenyang University of Technology, School of Science, Shenyang, ChinaThe biodegradation of polycyclic aromatic hydrocarbons (PAHs) in soils by laccase is reported. However, the low laccase activities of free laccase have limited its applications in environmental bioremediation. In this study, polluted soil was made with 3:1 volume ratio of soil to PAH solution. Subsequently, the adsorption–cross-link composite immobilization method was applied to immobilize laccase derived from fungi onto nylon net and chitosan, respectively. These two kinds of immobilized laccase were used in the degradation of pyrene (Pyr) and benzo[a]pyrene (BaP), and their degradation efficiencies under different temperature and pH conditions were investigated. Consequently, the optimal laboratory experimental parameters were determined as follows: first, compared with free laccase, the degradation rates of Pyr and BaP by immobilized laccase increased by around 10–30 %; second, the degradation efficiency of chitosan as a carrier of immobilized laccase was much better than that of nylon net as a carrier of the immobilized laccase; finally, when the temperature was set at 40°C and the pH was set at 4, the degradation efficiency achieved by immobilized laccase was the best.http://www.doiserbia.nb.rs/img/doi/0352-5139/2018/0352-51391800022W.pdfimmobilized laccasepolycyclic aromatic hydrocarbons (PAHs)soil remediationnylon netchitosan |
| spellingShingle | Wang Xin Shi Yu Sun Ni Zi Jun Li Zhao Xing Bao Jia Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase Journal of the Serbian Chemical Society immobilized laccase polycyclic aromatic hydrocarbons (PAHs) soil remediation nylon net chitosan |
| title | Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase |
| title_full | Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase |
| title_fullStr | Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase |
| title_full_unstemmed | Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase |
| title_short | Degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase |
| title_sort | degradation of polycyclic aromatic hydrocarbons in contaminated soil by immobilized laccase |
| topic | immobilized laccase polycyclic aromatic hydrocarbons (PAHs) soil remediation nylon net chitosan |
| url | http://www.doiserbia.nb.rs/img/doi/0352-5139/2018/0352-51391800022W.pdf |
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