Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System
In the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system. PGCs cultured for 465 days were transfected in vitro, and GFP gene expression was observed in 25% of the treated PGCs after culturing for furthe...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Japan Poultry Science Association
2015-07-01
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| Series: | The Journal of Poultry Science |
| Subjects: | |
| Online Access: | https://www.jstage.jst.go.jp/article/jpsa/52/3/52_0140197/_pdf/-char/en |
| _version_ | 1827938920200929280 |
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| author | Mitsuru Naito Takashi Harumi Takashi Kuwana |
| author_facet | Mitsuru Naito Takashi Harumi Takashi Kuwana |
| author_sort | Mitsuru Naito |
| collection | DOAJ |
| description | In the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system. PGCs cultured for 465 days were transfected in vitro, and GFP gene expression was observed in 25% of the treated PGCs after culturing for further 42 days. The cultured PGCs expressing GFP gene were transferred to recipient embryos and strong GFP gene expression was observed in the recipient gonads at day 18.5 of incubation. Circulating PGCs were transfected in vivo, and intense GFP gene expression was observed in the gonads of recipient embryos at day 18.5 of incubation. The procedure employed in the present study will contribute to successful gene transfer into chickens. |
| first_indexed | 2024-03-13T08:45:48Z |
| format | Article |
| id | doaj.art-1d3d76c97f8d420ea7c659579a40f462 |
| institution | Directory Open Access Journal |
| issn | 1346-7395 1349-0486 |
| language | English |
| last_indexed | 2024-03-13T08:45:48Z |
| publishDate | 2015-07-01 |
| publisher | Japan Poultry Science Association |
| record_format | Article |
| series | The Journal of Poultry Science |
| spelling | doaj.art-1d3d76c97f8d420ea7c659579a40f4622023-05-30T04:33:40ZengJapan Poultry Science AssociationThe Journal of Poultry Science1346-73951349-04862015-07-0152322723110.2141/jpsa.0140197jpsaExpression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector SystemMitsuru Naito0Takashi Harumi1Takashi Kuwana2National Institute of Agrobiological Sciences, JapanNational Institute of Agrobiological Sciences, JapanInternational Institute of Avian Conservation Science, United Arab EmiratesIn the present study, chicken primordial germ cells (PGCs) were transfected with GFP gene in vitro or in vivo using the piggyBac transposon vector system. PGCs cultured for 465 days were transfected in vitro, and GFP gene expression was observed in 25% of the treated PGCs after culturing for further 42 days. The cultured PGCs expressing GFP gene were transferred to recipient embryos and strong GFP gene expression was observed in the recipient gonads at day 18.5 of incubation. Circulating PGCs were transfected in vivo, and intense GFP gene expression was observed in the gonads of recipient embryos at day 18.5 of incubation. The procedure employed in the present study will contribute to successful gene transfer into chickens.https://www.jstage.jst.go.jp/article/jpsa/52/3/52_0140197/_pdf/-char/enchick embryogfp geneprimordial germ cellstransfectiontransposon |
| spellingShingle | Mitsuru Naito Takashi Harumi Takashi Kuwana Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System The Journal of Poultry Science chick embryo gfp gene primordial germ cells transfection transposon |
| title | Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System |
| title_full | Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System |
| title_fullStr | Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System |
| title_full_unstemmed | Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System |
| title_short | Expression of GFP Gene in Gonads of Chicken Embryos by Transfecting Primordial Germ Cells in vitro or in vivo using the PiggyBac Transposon Vector System |
| title_sort | expression of gfp gene in gonads of chicken embryos by transfecting primordial germ cells in vitro or in vivo using the piggybac transposon vector system |
| topic | chick embryo gfp gene primordial germ cells transfection transposon |
| url | https://www.jstage.jst.go.jp/article/jpsa/52/3/52_0140197/_pdf/-char/en |
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