Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppression
There is great interest in evaluating the anti-inflammatory properties of new herbal products. Thus, the effects of Mentha pulegium L. extract on gene and protein expressions of pro-inflammatory mediators and transcription factors were determined.The hydro-ethanolic extract of Mentha pulegium L. was...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-01-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024000719 |
| _version_ | 1797336876509560832 |
|---|---|
| author | Firouz Mohammadi Kaveh Rahimi Abbas Ahmadi Zahra Hooshmandi Sabrieh Amini Asadollah Mohammadi |
| author_facet | Firouz Mohammadi Kaveh Rahimi Abbas Ahmadi Zahra Hooshmandi Sabrieh Amini Asadollah Mohammadi |
| author_sort | Firouz Mohammadi |
| collection | DOAJ |
| description | There is great interest in evaluating the anti-inflammatory properties of new herbal products. Thus, the effects of Mentha pulegium L. extract on gene and protein expressions of pro-inflammatory mediators and transcription factors were determined.The hydro-ethanolic extract of Mentha pulegium L. was obtained and optimal non-cytotoxic concentrations of the extract were determined by MTT assay. Then, three different concentrations of Mentha pulegium L. (10, 30, and 90 μg/mL) were used to pre-treat the lipopolysaccharide (LPS)-stimulated and non-stimulated peripheral blood mononuclear cells (PBMCs) of 10 healthy individuals. Finally, the tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, Toll-like receptor-4 (TLR-4), nuclear factor-kappa B (NF-κB) p65, activator protein-1 (AP-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) gene expressions and TNF-α, IL-1β, IL-6, TLR-4, prostaglandin E2 (PGE2), and COX-2 protein levels were measured.MTT results showed that there is no significant difference in cell viability among 10, 20, 40, and 80 μg/mL concentrations of Mentha pulegium L. extract at 24, 48, and 72 h (P > 0.05). The IC50 values were 236.1, 147.0, and 118.0 μg/mL after 24, 48, and 72 h respectively. TNF-α, IL-1β, IL-6, TLR-4, iNOS, and NF-κB p65 mRNA levels in the pre-treated LPS-stimulated PBMCs were concentration-dependently reduced (P < 0.01 for TNF-α, TLR-4, and NF-κB p65; P < 0.05 for IL-1β, IL-6, and iNOS). Also, the protein levels of pro-inflammatory mediators decreased and these differences were significant for TNF-α, IL-1β, and TLR-4 (P < 0.001, P < 0.01, and P < 0.001, respectively).Mentha pulegium L. extract decreased the expression and biosynthesis of pro-inflammatory mediators. These effects are mainly mediated by TLR-4 and NF-κB suppression. Thus, Mentha pulegium L. could be useful in treating or ameliorating chronic inflammatory diseases. |
| first_indexed | 2024-03-08T09:01:16Z |
| format | Article |
| id | doaj.art-1d3def091d3e47f0a54f811be9a23589 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-08T09:01:16Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-1d3def091d3e47f0a54f811be9a235892024-02-01T06:34:29ZengElsevierHeliyon2405-84402024-01-01101e24040Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppressionFirouz Mohammadi0Kaveh Rahimi1Abbas Ahmadi2Zahra Hooshmandi3Sabrieh Amini4Asadollah Mohammadi5Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, IranDepartment of Basic Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, IranCellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, IranDepartment of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, IranDepartment of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran; Corresponding author. Department of Biology, Sanandaj Branch, Islamic Azad University, Pasdaran Ave., Sanandaj, Iran.Cellular and Molecular Research Center, Research Institute for Health Development, Kurdistan University of Medical Sciences, Sanandaj, Iran; Corresponding author.There is great interest in evaluating the anti-inflammatory properties of new herbal products. Thus, the effects of Mentha pulegium L. extract on gene and protein expressions of pro-inflammatory mediators and transcription factors were determined.The hydro-ethanolic extract of Mentha pulegium L. was obtained and optimal non-cytotoxic concentrations of the extract were determined by MTT assay. Then, three different concentrations of Mentha pulegium L. (10, 30, and 90 μg/mL) were used to pre-treat the lipopolysaccharide (LPS)-stimulated and non-stimulated peripheral blood mononuclear cells (PBMCs) of 10 healthy individuals. Finally, the tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6, Toll-like receptor-4 (TLR-4), nuclear factor-kappa B (NF-κB) p65, activator protein-1 (AP-1), inducible nitric oxide synthase (iNOS), and cyclooxygenase-2 (COX-2) gene expressions and TNF-α, IL-1β, IL-6, TLR-4, prostaglandin E2 (PGE2), and COX-2 protein levels were measured.MTT results showed that there is no significant difference in cell viability among 10, 20, 40, and 80 μg/mL concentrations of Mentha pulegium L. extract at 24, 48, and 72 h (P > 0.05). The IC50 values were 236.1, 147.0, and 118.0 μg/mL after 24, 48, and 72 h respectively. TNF-α, IL-1β, IL-6, TLR-4, iNOS, and NF-κB p65 mRNA levels in the pre-treated LPS-stimulated PBMCs were concentration-dependently reduced (P < 0.01 for TNF-α, TLR-4, and NF-κB p65; P < 0.05 for IL-1β, IL-6, and iNOS). Also, the protein levels of pro-inflammatory mediators decreased and these differences were significant for TNF-α, IL-1β, and TLR-4 (P < 0.001, P < 0.01, and P < 0.001, respectively).Mentha pulegium L. extract decreased the expression and biosynthesis of pro-inflammatory mediators. These effects are mainly mediated by TLR-4 and NF-κB suppression. Thus, Mentha pulegium L. could be useful in treating or ameliorating chronic inflammatory diseases.http://www.sciencedirect.com/science/article/pii/S2405844024000719InflammationHerbal medicineMentha pulegiumAnti-Inflammatory effects |
| spellingShingle | Firouz Mohammadi Kaveh Rahimi Abbas Ahmadi Zahra Hooshmandi Sabrieh Amini Asadollah Mohammadi Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppression Heliyon Inflammation Herbal medicine Mentha pulegium Anti-Inflammatory effects |
| title | Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppression |
| title_full | Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppression |
| title_fullStr | Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppression |
| title_full_unstemmed | Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppression |
| title_short | Anti-inflammatory effects of Mentha pulegium L. extract on human peripheral blood mononuclear cells are mediated by TLR-4 and NF-κB suppression |
| title_sort | anti inflammatory effects of mentha pulegium l extract on human peripheral blood mononuclear cells are mediated by tlr 4 and nf κb suppression |
| topic | Inflammation Herbal medicine Mentha pulegium Anti-Inflammatory effects |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024000719 |
| work_keys_str_mv | AT firouzmohammadi antiinflammatoryeffectsofmenthapulegiumlextractonhumanperipheralbloodmononuclearcellsaremediatedbytlr4andnfkbsuppression AT kavehrahimi antiinflammatoryeffectsofmenthapulegiumlextractonhumanperipheralbloodmononuclearcellsaremediatedbytlr4andnfkbsuppression AT abbasahmadi antiinflammatoryeffectsofmenthapulegiumlextractonhumanperipheralbloodmononuclearcellsaremediatedbytlr4andnfkbsuppression AT zahrahooshmandi antiinflammatoryeffectsofmenthapulegiumlextractonhumanperipheralbloodmononuclearcellsaremediatedbytlr4andnfkbsuppression AT sabriehamini antiinflammatoryeffectsofmenthapulegiumlextractonhumanperipheralbloodmononuclearcellsaremediatedbytlr4andnfkbsuppression AT asadollahmohammadi antiinflammatoryeffectsofmenthapulegiumlextractonhumanperipheralbloodmononuclearcellsaremediatedbytlr4andnfkbsuppression |