An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method
Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-01-01
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| Series: | Diagnostics |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2075-4418/4/1/1 |
| _version_ | 1818035265891991552 |
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| author | Frank Eertmans Veerle Bogaert Tanita Van Poecke Barbara Puype |
| author_facet | Frank Eertmans Veerle Bogaert Tanita Van Poecke Barbara Puype |
| author_sort | Frank Eertmans |
| collection | DOAJ |
| description | Neutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen. |
| first_indexed | 2024-12-10T06:52:19Z |
| format | Article |
| id | doaj.art-1d3e291e331642ab9d9cc088b8c9c0a7 |
| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-12-10T06:52:19Z |
| publishDate | 2014-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-1d3e291e331642ab9d9cc088b8c9c0a72022-12-22T01:58:30ZengMDPI AGDiagnostics2075-44182014-01-014111110.3390/diagnostics4010001diagnostics4010001An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO MethodFrank Eertmans0Veerle Bogaert1Tanita Van Poecke2Barbara Puype3Department of Research and Development, FertiPro NV, Industriepark Noord 32, 8730 Beernem, WVL, BelgiumDepartment of Research and Development, FertiPro NV, Industriepark Noord 32, 8730 Beernem, WVL, BelgiumLaboratory of Andrology, Ghent University Hospital, De Pintelaan 185, 9000 Ghent, OVL, BelgiumDepartment of Research and Development, FertiPro NV, Industriepark Noord 32, 8730 Beernem, WVL, BelgiumNeutral a-glucosidase (NAG) activity in human seminal plasma is an important indicator for epididymis functionality. In the present study, the classic World Health Organization (WHO) method has been adapted to enhance assay robustness. Changes include modified enzyme reaction buffer composition and usage of an alternative enzyme inhibitor for background correction (glucose instead of castanospermine). Both methods have been tested in parallel on 144 semen samples, obtained from 94 patients/donors and 50 vasectomized men (negative control), respectively. Passing-Bablok regression analysis demonstrated equal assay performance. In terms of assay validation, analytical specificity, detection limit, measuring range, precision, and cut-off values have been calculated. These data confirm that the adapted method is a reliable, improved tool for NAG analysis in human semen.http://www.mdpi.com/2075-4418/4/1/1epididymisneutral a-glucosidasemethod optimizationassay validation |
| spellingShingle | Frank Eertmans Veerle Bogaert Tanita Van Poecke Barbara Puype An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method Diagnostics epididymis neutral a-glucosidase method optimization assay validation |
| title | An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method |
| title_full | An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method |
| title_fullStr | An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method |
| title_full_unstemmed | An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method |
| title_short | An Improved Neutral a-Glucosidase Assay for Assessment of Epididymal Function—Validation and Comparison to the WHO Method |
| title_sort | improved neutral a glucosidase assay for assessment of epididymal function validation and comparison to the who method |
| topic | epididymis neutral a-glucosidase method optimization assay validation |
| url | http://www.mdpi.com/2075-4418/4/1/1 |
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