High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer
Combining focused optical excitation and high-frequency ultrasound detection, optical-resolution photoacoustic microscopy (OR-PAM) can provide micrometer-level spatial resolution with millimeter-level penetration depth and has been employed in a variety of biomedical applications. However, it remain...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Photoacoustics |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213597922000829 |
| _version_ | 1811300234197729280 |
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| author | Maomao Chen Laiming Jiang Clare Cook Yushun Zeng Tri Vu Ruimin Chen Gengxi Lu Wei Yang Ulrike Hoffmann Qifa Zhou Junjie Yao |
| author_facet | Maomao Chen Laiming Jiang Clare Cook Yushun Zeng Tri Vu Ruimin Chen Gengxi Lu Wei Yang Ulrike Hoffmann Qifa Zhou Junjie Yao |
| author_sort | Maomao Chen |
| collection | DOAJ |
| description | Combining focused optical excitation and high-frequency ultrasound detection, optical-resolution photoacoustic microscopy (OR-PAM) can provide micrometer-level spatial resolution with millimeter-level penetration depth and has been employed in a variety of biomedical applications. However, it remains a challenge for OR-PAM to achieve a high imaging speed and a large field of view at the same time. In this work, we report a new approach to implement high-speed wide-field OR-PAM, using a cylindrically-focused transparent ultrasound transducer (CFT-UT). The CFT-UT is made of transparent lithium niobate coated with indium-tin-oxide as electrodes. A transparent cylindrical lens is attached to the transducer surface to provide an acoustic focal line with a length of 9 mm. The excitation light can pass directly through the CFT-UT from the above and thus enables a reflection imaging mode. High-speed imaging is achieved by fast optical scanning of the focused excitation light along the CFT-UT focal line. With the confocal alignment of the optical excitation and acoustic detection, a relatively high detection sensitivity is maintained over the entire scanning range. The CFT-UT-based OR-PAM system has achieved a cross-sectional frame rate of 500 Hz over the scanning range of 9 mm. We have characterized the system’s performance on phantoms and demonstrated its application on small animal models in vivo. We expect the new CFT-UT-based OR-PAM will find matched biomedical applications that need high imaging speed over a large field of view. |
| first_indexed | 2024-04-13T06:48:10Z |
| format | Article |
| id | doaj.art-1d3eff966c514bfeb40a40ba86da79f9 |
| institution | Directory Open Access Journal |
| issn | 2213-5979 |
| language | English |
| last_indexed | 2024-04-13T06:48:10Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Photoacoustics |
| spelling | doaj.art-1d3eff966c514bfeb40a40ba86da79f92022-12-22T02:57:29ZengElsevierPhotoacoustics2213-59792022-12-0128100417High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducerMaomao Chen0Laiming Jiang1Clare Cook2Yushun Zeng3Tri Vu4Ruimin Chen5Gengxi Lu6Wei Yang7Ulrike Hoffmann8Qifa Zhou9Junjie Yao10Department of Biomedical Engineering, Duke University, Durham, NC 27708, USADepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USADepartment of Biomedical Engineering, Duke University, Durham, NC 27708, USADepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USADepartment of Biomedical Engineering, Duke University, Durham, NC 27708, USADepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USADepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USAMultidisciplinary Brain Protection Program, Department of Anaesthesiology, Duke University School of Medicine, Durham, NC 27708, USAMultidisciplinary Brain Protection Program, Department of Anaesthesiology, Duke University School of Medicine, Durham, NC 27708, USADepartment of Biomedical Engineering, University of Southern California, Los Angeles, CA 90089, USA; Corresponding authors.Department of Biomedical Engineering, Duke University, Durham, NC 27708, USA; Corresponding authors.Combining focused optical excitation and high-frequency ultrasound detection, optical-resolution photoacoustic microscopy (OR-PAM) can provide micrometer-level spatial resolution with millimeter-level penetration depth and has been employed in a variety of biomedical applications. However, it remains a challenge for OR-PAM to achieve a high imaging speed and a large field of view at the same time. In this work, we report a new approach to implement high-speed wide-field OR-PAM, using a cylindrically-focused transparent ultrasound transducer (CFT-UT). The CFT-UT is made of transparent lithium niobate coated with indium-tin-oxide as electrodes. A transparent cylindrical lens is attached to the transducer surface to provide an acoustic focal line with a length of 9 mm. The excitation light can pass directly through the CFT-UT from the above and thus enables a reflection imaging mode. High-speed imaging is achieved by fast optical scanning of the focused excitation light along the CFT-UT focal line. With the confocal alignment of the optical excitation and acoustic detection, a relatively high detection sensitivity is maintained over the entire scanning range. The CFT-UT-based OR-PAM system has achieved a cross-sectional frame rate of 500 Hz over the scanning range of 9 mm. We have characterized the system’s performance on phantoms and demonstrated its application on small animal models in vivo. We expect the new CFT-UT-based OR-PAM will find matched biomedical applications that need high imaging speed over a large field of view.http://www.sciencedirect.com/science/article/pii/S2213597922000829High-speed imagingWide-field imagingOptical-resolution photoacoustic microscopyCylindrically-focused ultrasound transducerTransparent ultrasound transducerBrain imaging |
| spellingShingle | Maomao Chen Laiming Jiang Clare Cook Yushun Zeng Tri Vu Ruimin Chen Gengxi Lu Wei Yang Ulrike Hoffmann Qifa Zhou Junjie Yao High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer Photoacoustics High-speed imaging Wide-field imaging Optical-resolution photoacoustic microscopy Cylindrically-focused ultrasound transducer Transparent ultrasound transducer Brain imaging |
| title | High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer |
| title_full | High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer |
| title_fullStr | High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer |
| title_full_unstemmed | High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer |
| title_short | High-speed wide-field photoacoustic microscopy using a cylindrically focused transparent high-frequency ultrasound transducer |
| title_sort | high speed wide field photoacoustic microscopy using a cylindrically focused transparent high frequency ultrasound transducer |
| topic | High-speed imaging Wide-field imaging Optical-resolution photoacoustic microscopy Cylindrically-focused ultrasound transducer Transparent ultrasound transducer Brain imaging |
| url | http://www.sciencedirect.com/science/article/pii/S2213597922000829 |
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