Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged Fermentation
Cellulase enzymes attract a lot of research due to their industrial application. Diverse cellulase-producing organisms and substances that induce cellulase are highly sought after. This study aimed to evaluate the effect of different inducer sources on cellulase production by white rot fungi <i&g...
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MDPI AG
2022-10-01
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| Series: | Fermentation |
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| Online Access: | https://www.mdpi.com/2311-5637/8/10/561 |
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| author | Osmond Datsomor Qi Yan Louis Opoku-Mensah Guoqi Zhao Lin Miao |
| author_facet | Osmond Datsomor Qi Yan Louis Opoku-Mensah Guoqi Zhao Lin Miao |
| author_sort | Osmond Datsomor |
| collection | DOAJ |
| description | Cellulase enzymes attract a lot of research due to their industrial application. Diverse cellulase-producing organisms and substances that induce cellulase are highly sought after. This study aimed to evaluate the effect of different inducer sources on cellulase production by white rot fungi <i>P</i>. <i>ostreatus</i> CGMCC 3.7292 and <i>P</i>. <i>chrysosporium</i> CGMCC 3.7212 under submerged fermentation employing a completely randomized experimental design. The different inducer sources tested were nitrogen (yeast, potassium nitrate, sodium nitrate, ammonium sulphate, aqueous ammonia and urea), carbon (malt extract, glucose, fructose, carboxymethylcellulose, starch and xylose) and agro-biomass (stevia straw, wheat straw, oat straw, alfalfa straw, corn cobs and corn stover). These inducer sources strongly impacted enzyme activities by <i>P</i>. <i>ostreatus</i> CGMCC 3.7292 and <i>P</i>. <i>chrysosporium</i> CGMCC 3.7212. The suitable nitrogen and carbon inducer sources for cellulase activity by <i>P</i>. <i>ostreatus</i> and <i>P</i>. <i>chrysosporium</i> were yeast (1.354 U/mL and 1.154 U/mL) and carboxymethylcellulose (0.976 U/mL and 0.776 U/mL) while the suitable agro-biomass were wheat straw (6.880 U/mL) and corn stover (6.525 U/mL), respectively. The least inducer sources in terms of nitrogen, carbon and agro-biomass for cellulase activity by <i>P</i>. <i>ostreatus</i> and <i>P</i>. <i>chrysosporium</i> were urea (0.213 U/mL and 0.081 U/mL), glucose (0.042 U/mL and 0.035), xylose (0.042 U/mL and 0.035 U/mL) and stevia straw (1.555 U/mL and 0.960 U/mL). In submerged fermentation, the cellulase enzyme activity of <i>P</i>. ostreatus in response to various inducer sources was relatively higher than <i>P</i>. <i>chrysosporium</i>. |
| first_indexed | 2024-03-09T03:40:06Z |
| format | Article |
| id | doaj.art-1d40f4092df84704abb53ccd2f8151eb |
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| last_indexed | 2024-03-09T03:40:06Z |
| publishDate | 2022-10-01 |
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| spelling | doaj.art-1d40f4092df84704abb53ccd2f8151eb2023-12-03T14:42:01ZengMDPI AGFermentation2311-56372022-10-0181056110.3390/fermentation8100561Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged FermentationOsmond Datsomor0Qi Yan1Louis Opoku-Mensah2Guoqi Zhao3Lin Miao4Department of Animal Nutrition and Feed Science, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaNational Sheep Breeding Station, Animal Production Department, Ministry of Food and Agriculture, P.O. Box EA33, Ejura 3223, GhanaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaDepartment of Animal Nutrition and Feed Science, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, ChinaCellulase enzymes attract a lot of research due to their industrial application. Diverse cellulase-producing organisms and substances that induce cellulase are highly sought after. This study aimed to evaluate the effect of different inducer sources on cellulase production by white rot fungi <i>P</i>. <i>ostreatus</i> CGMCC 3.7292 and <i>P</i>. <i>chrysosporium</i> CGMCC 3.7212 under submerged fermentation employing a completely randomized experimental design. The different inducer sources tested were nitrogen (yeast, potassium nitrate, sodium nitrate, ammonium sulphate, aqueous ammonia and urea), carbon (malt extract, glucose, fructose, carboxymethylcellulose, starch and xylose) and agro-biomass (stevia straw, wheat straw, oat straw, alfalfa straw, corn cobs and corn stover). These inducer sources strongly impacted enzyme activities by <i>P</i>. <i>ostreatus</i> CGMCC 3.7292 and <i>P</i>. <i>chrysosporium</i> CGMCC 3.7212. The suitable nitrogen and carbon inducer sources for cellulase activity by <i>P</i>. <i>ostreatus</i> and <i>P</i>. <i>chrysosporium</i> were yeast (1.354 U/mL and 1.154 U/mL) and carboxymethylcellulose (0.976 U/mL and 0.776 U/mL) while the suitable agro-biomass were wheat straw (6.880 U/mL) and corn stover (6.525 U/mL), respectively. The least inducer sources in terms of nitrogen, carbon and agro-biomass for cellulase activity by <i>P</i>. <i>ostreatus</i> and <i>P</i>. <i>chrysosporium</i> were urea (0.213 U/mL and 0.081 U/mL), glucose (0.042 U/mL and 0.035), xylose (0.042 U/mL and 0.035 U/mL) and stevia straw (1.555 U/mL and 0.960 U/mL). In submerged fermentation, the cellulase enzyme activity of <i>P</i>. ostreatus in response to various inducer sources was relatively higher than <i>P</i>. <i>chrysosporium</i>.https://www.mdpi.com/2311-5637/8/10/561submerged fermentation<i>P. ostreatus</i><i>P. chrysosporium</i>inducer sourcesagro-biomasscellulase enzyme |
| spellingShingle | Osmond Datsomor Qi Yan Louis Opoku-Mensah Guoqi Zhao Lin Miao Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged Fermentation Fermentation submerged fermentation <i>P. ostreatus</i> <i>P. chrysosporium</i> inducer sources agro-biomass cellulase enzyme |
| title | Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged Fermentation |
| title_full | Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged Fermentation |
| title_fullStr | Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged Fermentation |
| title_full_unstemmed | Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged Fermentation |
| title_short | Effect of Different Inducer Sources on Cellulase Enzyme Production by White-Rot Basidiomycetes <i>Pleurotus ostreatus</i> and <i>Phanerochaete chrysosporium</i> under Submerged Fermentation |
| title_sort | effect of different inducer sources on cellulase enzyme production by white rot basidiomycetes i pleurotus ostreatus i and i phanerochaete chrysosporium i under submerged fermentation |
| topic | submerged fermentation <i>P. ostreatus</i> <i>P. chrysosporium</i> inducer sources agro-biomass cellulase enzyme |
| url | https://www.mdpi.com/2311-5637/8/10/561 |
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