Improving Thermostability of GH11 Xylanase XynASP by the Design of Loop Region

Improving Thermostability of GH11 Xylanase XynASP by the Design of Loop Region

Highly flexible loop regions can affect the structure-function relationship of enzymes. In this study, to reveal the effect of the loop on the thermal stability of GH11 xylanase XynASP from <i>Aspergillus saccharolyticus</i> JOP 1030-1, three mutants (T41V, A79Y, T81Q) located in the loo...

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Bibliographic Details
Main Authors: Tongbiao Li, Siwen Yang, Xiaoxiao Wang, Hongxuan Cai, Ye Wang, Chao Li, Enzhong Li
Format: Article
Language:English
Published: MDPI AG 2022-08-01
Series:Crystals
Subjects:
GH11 xylanase
thermostability
loop
site-directed mutagenesis
protein engineering
Online Access:https://www.mdpi.com/2073-4352/12/9/1228

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