Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles
Abstract Background Magnetic nanoparticles (MNPs) are characterized by unique physicochemical and biological properties that allow their employment as highly biocompatible drug carriers. Gelsolin (GSN) is a multifunctional actin-binding protein involved in cytoskeleton remodeling and free circulatin...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-07-01
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| Series: | Journal of Nanobiotechnology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12951-019-0511-1 |
| _version_ | 1817993388699418624 |
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| author | Robert Bucki Katarzyna Niemirowicz-Laskowska Piotr Deptuła Agnieszka Z. Wilczewska Paweł Misiak Bonita Durnaś Krzysztof Fiedoruk Ewelina Piktel Joanna Mystkowska Paul A. Janmey |
| author_facet | Robert Bucki Katarzyna Niemirowicz-Laskowska Piotr Deptuła Agnieszka Z. Wilczewska Paweł Misiak Bonita Durnaś Krzysztof Fiedoruk Ewelina Piktel Joanna Mystkowska Paul A. Janmey |
| author_sort | Robert Bucki |
| collection | DOAJ |
| description | Abstract Background Magnetic nanoparticles (MNPs) are characterized by unique physicochemical and biological properties that allow their employment as highly biocompatible drug carriers. Gelsolin (GSN) is a multifunctional actin-binding protein involved in cytoskeleton remodeling and free circulating actin sequestering. It was reported that a gelsolin derived phosphoinositide binding domain GSN 160–169, (PBP10 peptide) coupled with rhodamine B, exerts strong bactericidal activity. Results In this study, we synthesized a new antibacterial and antifungal nanosystem composed of MNPs and a PBP10 peptide attached to the surface. The physicochemical properties of these nanosystems were analyzed by spectroscopy, calorimetry, electron microscopy, and X-ray studies. Using luminescence based techniques and a standard killing assay against representative strains of Gram-positive (Staphylococcus aureus MRSA Xen 30) and Gram-negative (Pseudomonas aeruginosa Xen 5) bacteria and against fungal cells (Candida spp.) we demonstrated that magnetic nanoparticles significantly enhance the effect of PBP10 peptides through a membrane-based mode of action, involving attachment and interaction with cell wall components, disruption of microbial membrane and increased uptake of peptide. Our results also indicate that treatment of both planktonic and biofilm forms of pathogens by PBP10-based nanosystems is more effective than therapy with either of these agents alone. Conclusions The results show that magnetic nanoparticles enhance the antimicrobial activity of the phosphoinositide-binding domain of gelsolin, modulate its mode of action and strengthen the idea of its employment for developing the new treatment methods of infections. |
| first_indexed | 2024-04-14T01:39:13Z |
| format | Article |
| id | doaj.art-1d44089628d64e4aaebb20a934cadddd |
| institution | Directory Open Access Journal |
| issn | 1477-3155 |
| language | English |
| last_indexed | 2024-04-14T01:39:13Z |
| publishDate | 2019-07-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Nanobiotechnology |
| spelling | doaj.art-1d44089628d64e4aaebb20a934cadddd2022-12-22T02:19:49ZengBMCJournal of Nanobiotechnology1477-31552019-07-0117111510.1186/s12951-019-0511-1Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticlesRobert Bucki0Katarzyna Niemirowicz-Laskowska1Piotr Deptuła2Agnieszka Z. Wilczewska3Paweł Misiak4Bonita Durnaś5Krzysztof Fiedoruk6Ewelina Piktel7Joanna Mystkowska8Paul A. Janmey9Department of Medical Microbiology and Nanobiomedical Engineering, Medical University of BialystokDepartment of Medical Microbiology and Nanobiomedical Engineering, Medical University of BialystokDepartment of Medical Microbiology and Nanobiomedical Engineering, Medical University of BialystokInstitute of Chemistry, University of BiałystokInstitute of Chemistry, University of BiałystokDepartment of Microbiology and Immunology, The Faculty of Medicine and Health Sciences of the Jan Kochanowski University in KielceDepartment of Microbiology, Medical University of BialystokDepartment of Medical Microbiology and Nanobiomedical Engineering, Medical University of BialystokDepartment of Materials Engineering and Production, Faculty of Mechanical Engineering, Bialystok University of TechnologyDepartment of Physiology and Institute for Medicine and Engineering, University of PennsylvaniaAbstract Background Magnetic nanoparticles (MNPs) are characterized by unique physicochemical and biological properties that allow their employment as highly biocompatible drug carriers. Gelsolin (GSN) is a multifunctional actin-binding protein involved in cytoskeleton remodeling and free circulating actin sequestering. It was reported that a gelsolin derived phosphoinositide binding domain GSN 160–169, (PBP10 peptide) coupled with rhodamine B, exerts strong bactericidal activity. Results In this study, we synthesized a new antibacterial and antifungal nanosystem composed of MNPs and a PBP10 peptide attached to the surface. The physicochemical properties of these nanosystems were analyzed by spectroscopy, calorimetry, electron microscopy, and X-ray studies. Using luminescence based techniques and a standard killing assay against representative strains of Gram-positive (Staphylococcus aureus MRSA Xen 30) and Gram-negative (Pseudomonas aeruginosa Xen 5) bacteria and against fungal cells (Candida spp.) we demonstrated that magnetic nanoparticles significantly enhance the effect of PBP10 peptides through a membrane-based mode of action, involving attachment and interaction with cell wall components, disruption of microbial membrane and increased uptake of peptide. Our results also indicate that treatment of both planktonic and biofilm forms of pathogens by PBP10-based nanosystems is more effective than therapy with either of these agents alone. Conclusions The results show that magnetic nanoparticles enhance the antimicrobial activity of the phosphoinositide-binding domain of gelsolin, modulate its mode of action and strengthen the idea of its employment for developing the new treatment methods of infections.http://link.springer.com/article/10.1186/s12951-019-0511-1GelsolinPBP10 peptideMagnetic nanoparticlesFungal cellsAntibacterial |
| spellingShingle | Robert Bucki Katarzyna Niemirowicz-Laskowska Piotr Deptuła Agnieszka Z. Wilczewska Paweł Misiak Bonita Durnaś Krzysztof Fiedoruk Ewelina Piktel Joanna Mystkowska Paul A. Janmey Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles Journal of Nanobiotechnology Gelsolin PBP10 peptide Magnetic nanoparticles Fungal cells Antibacterial |
| title | Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles |
| title_full | Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles |
| title_fullStr | Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles |
| title_full_unstemmed | Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles |
| title_short | Susceptibility of microbial cells to the modified PIP2-binding sequence of gelsolin anchored on the surface of magnetic nanoparticles |
| title_sort | susceptibility of microbial cells to the modified pip2 binding sequence of gelsolin anchored on the surface of magnetic nanoparticles |
| topic | Gelsolin PBP10 peptide Magnetic nanoparticles Fungal cells Antibacterial |
| url | http://link.springer.com/article/10.1186/s12951-019-0511-1 |
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