Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability
Methanothermobacter thermautotrophicus strain ∆H is a model hydrogenotrophic methanogen, for which extensive biochemical information, including the complete genome sequence, is available. Nevertheless, at the cell membrane lipid level, little is known about the responses of this archaeon to environm...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2015-01-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00005/full |
| _version_ | 1818310466696380416 |
|---|---|
| author | Marcos Yukio Yoshinaga Emma J. Gagen Emma J. Gagen Lars eWörmer Nadine K. Broda Travis Blake Meador Jenny eWendt Michael eThomm Kai-Uwe eHinrichs |
| author_facet | Marcos Yukio Yoshinaga Emma J. Gagen Emma J. Gagen Lars eWörmer Nadine K. Broda Travis Blake Meador Jenny eWendt Michael eThomm Kai-Uwe eHinrichs |
| author_sort | Marcos Yukio Yoshinaga |
| collection | DOAJ |
| description | Methanothermobacter thermautotrophicus strain ∆H is a model hydrogenotrophic methanogen, for which extensive biochemical information, including the complete genome sequence, is available. Nevertheless, at the cell membrane lipid level, little is known about the responses of this archaeon to environmental stimuli. In this study, the lipid composition of M. thermautotrophicus was characterized to verify how this archaeon modulates its cell membrane components during growth phases and in response to hydrogen depletion and nutrient limitation (potassium and phosphate). As opposed to the higher abundance of phospholipids in the stationary phase of control experiments, cell membranes under nutrient and energy stress were dominated by glycolipids that likely provided a more effective barrier against ion leakage. We also identified particular lipid regulatory mechanisms in M. thermautotrophicus, which included the accumulation of polyprenols under hydrogen-limited conditions and an increased content of sodiated adducts of lipids in nutrient-limited cells. These findings suggest that M. thermautotrophicus intensely modulates its cell membrane lipid composition to cope with energy and nutrient availability in dynamic environments. |
| first_indexed | 2024-12-13T07:46:31Z |
| format | Article |
| id | doaj.art-1d44e85cf7b1459280fadf434ebada43 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T07:46:31Z |
| publishDate | 2015-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1d44e85cf7b1459280fadf434ebada432022-12-21T23:54:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-01-01610.3389/fmicb.2015.00005126069Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availabilityMarcos Yukio Yoshinaga0Emma J. Gagen1Emma J. Gagen2Lars eWörmer3Nadine K. Broda4Travis Blake Meador5Jenny eWendt6Michael eThomm7Kai-Uwe eHinrichs8University of BremenUniversity of RegensburgUniversity of QueenslandUniversity of BremenUniversity of BremenUniversity of BremenUniversity of BremenUniversity of RegensburgUniversity of BremenMethanothermobacter thermautotrophicus strain ∆H is a model hydrogenotrophic methanogen, for which extensive biochemical information, including the complete genome sequence, is available. Nevertheless, at the cell membrane lipid level, little is known about the responses of this archaeon to environmental stimuli. In this study, the lipid composition of M. thermautotrophicus was characterized to verify how this archaeon modulates its cell membrane components during growth phases and in response to hydrogen depletion and nutrient limitation (potassium and phosphate). As opposed to the higher abundance of phospholipids in the stationary phase of control experiments, cell membranes under nutrient and energy stress were dominated by glycolipids that likely provided a more effective barrier against ion leakage. We also identified particular lipid regulatory mechanisms in M. thermautotrophicus, which included the accumulation of polyprenols under hydrogen-limited conditions and an increased content of sodiated adducts of lipids in nutrient-limited cells. These findings suggest that M. thermautotrophicus intensely modulates its cell membrane lipid composition to cope with energy and nutrient availability in dynamic environments.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00005/fullArchaeastress responsepolar lipidsDiethertetraether |
| spellingShingle | Marcos Yukio Yoshinaga Emma J. Gagen Emma J. Gagen Lars eWörmer Nadine K. Broda Travis Blake Meador Jenny eWendt Michael eThomm Kai-Uwe eHinrichs Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability Frontiers in Microbiology Archaea stress response polar lipids Diether tetraether |
| title | Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability |
| title_full | Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability |
| title_fullStr | Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability |
| title_full_unstemmed | Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability |
| title_short | Methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability |
| title_sort | methanothermobacter thermautotrophicus modulates its membrane lipids in response to hydrogen and nutrient availability |
| topic | Archaea stress response polar lipids Diether tetraether |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00005/full |
| work_keys_str_mv | AT marcosyukioyoshinaga methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT emmajgagen methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT emmajgagen methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT larsewormer methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT nadinekbroda methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT travisblakemeador methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT jennyewendt methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT michaelethomm methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability AT kaiuweehinrichs methanothermobacterthermautotrophicusmodulatesitsmembranelipidsinresponsetohydrogenandnutrientavailability |