Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest Iran
Background and Objectives: An increase in the antibiotic resistance of Shigella isolates has caused major global challenges in antimicrobial therapy. Knowledge of local antibiotic resistance trends is essential for selecting appropriate antibiotic treatment regimens. This study aimed to evaluate th...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2023-10-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4115 |
| _version_ | 1797658886746931200 |
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| author | Nabi Jomehzadeh Khadijeh Ahmadi Nazanin Ataee Maryam Afzali |
| author_facet | Nabi Jomehzadeh Khadijeh Ahmadi Nazanin Ataee Maryam Afzali |
| author_sort | Nabi Jomehzadeh |
| collection | DOAJ |
| description |
Background and Objectives: An increase in the antibiotic resistance of Shigella isolates has caused major global challenges in antimicrobial therapy. Knowledge of local antibiotic resistance trends is essential for selecting appropriate antibiotic treatment regimens. This study aimed to evaluate the frequency of efflux-mediated tetracycline resistance (tet) and plasmid-mediated quinolone resistance (qnr) genes among Shigella isolates.
Materials and Methods: This survey investigated 91 Shigella isolates, obtained from children with acute diarrhea. The isolates were identified using standard biochemical tests and confirmed by polymerase chain reaction (PCR) assay. Besides, the susceptibility of isolates to six selected antibiotics was assessed by the disk diffusion method. All tetracycline-resistant and nalidixic acid and ciprofloxacin resistant strains were screened for tet and qnr genes by a multiplex PCR assay.
Results: According to the results of antibiotic susceptibility tests, the highest level of antibiotic resistance was related to tetracycline (80.2%) and doxycycline (78.1%), respectively. All isolates were sensitive to tigecycline. The PCR results showed that 40.6%, 3.1%, 21.8%, 61.6% and 28.7% of the isolates carried qnrA, qnrB, qnrS, tetA, and tetB genes, respectively. None of the isolates contained tetC and tetD genes.
Conclusion: The current findings revealed that tetA and qnrA genes might play a key role in conferring tetracycline and quinolone resistance.
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| first_indexed | 2024-03-11T18:06:53Z |
| format | Article |
| id | doaj.art-1d454af4ddf945b6b8eed39d059d1c52 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-03-11T18:06:53Z |
| publishDate | 2023-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-1d454af4ddf945b6b8eed39d059d1c522023-10-17T05:23:24ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472023-10-0115510.18502/ijm.v15i5.13868Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest IranNabi Jomehzadeh0Khadijeh Ahmadi1Nazanin Ataee2Maryam Afzali3Department of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, IranDepartment of Microbiology, School of Medicine, Abadan University of Medical Sciences, Abadan, IranDepartment of Biology, Kavian Institute of Higher Education, Mashhad, IranDepartment of Microbiology, Faculty of Medicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran; Department of Laboratory Sciences, Faculty of Paramedicine, Mashhad Medical Sciences, Islamic Azad University, Mashhad, Iran Background and Objectives: An increase in the antibiotic resistance of Shigella isolates has caused major global challenges in antimicrobial therapy. Knowledge of local antibiotic resistance trends is essential for selecting appropriate antibiotic treatment regimens. This study aimed to evaluate the frequency of efflux-mediated tetracycline resistance (tet) and plasmid-mediated quinolone resistance (qnr) genes among Shigella isolates. Materials and Methods: This survey investigated 91 Shigella isolates, obtained from children with acute diarrhea. The isolates were identified using standard biochemical tests and confirmed by polymerase chain reaction (PCR) assay. Besides, the susceptibility of isolates to six selected antibiotics was assessed by the disk diffusion method. All tetracycline-resistant and nalidixic acid and ciprofloxacin resistant strains were screened for tet and qnr genes by a multiplex PCR assay. Results: According to the results of antibiotic susceptibility tests, the highest level of antibiotic resistance was related to tetracycline (80.2%) and doxycycline (78.1%), respectively. All isolates were sensitive to tigecycline. The PCR results showed that 40.6%, 3.1%, 21.8%, 61.6% and 28.7% of the isolates carried qnrA, qnrB, qnrS, tetA, and tetB genes, respectively. None of the isolates contained tetC and tetD genes. Conclusion: The current findings revealed that tetA and qnrA genes might play a key role in conferring tetracycline and quinolone resistance. https://ijm.tums.ac.ir/index.php/ijm/article/view/4115Diarrhea;Shigella;Tetracycline resistance;Quinolone resistance |
| spellingShingle | Nabi Jomehzadeh Khadijeh Ahmadi Nazanin Ataee Maryam Afzali Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest Iran Iranian Journal of Microbiology Diarrhea; Shigella; Tetracycline resistance; Quinolone resistance |
| title | Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest Iran |
| title_full | Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest Iran |
| title_fullStr | Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest Iran |
| title_full_unstemmed | Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest Iran |
| title_short | Molecular detection of genes encoding resistance to tetracycline and quinolones among Shigella strains isolated from children with acute diarrhea in southwest Iran |
| title_sort | molecular detection of genes encoding resistance to tetracycline and quinolones among shigella strains isolated from children with acute diarrhea in southwest iran |
| topic | Diarrhea; Shigella; Tetracycline resistance; Quinolone resistance |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4115 |
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