Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusion
Abstract Background Ischaemic stroke has become the main cause of death and severe neurological disorders, for which effective restorative treatments are currently limited. While stem cell transplantation offers therapeutic potential through neural regeneration, this approach is associated with the...
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| Format: | Article |
| Language: | English |
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BMC
2020-09-01
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| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-020-01927-w |
| _version_ | 1818274031441281024 |
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| author | Xuemei Zhang Hao Tang Senlin Mao Bing Li Yinglian Zhou Hui Yue Duo Wang Yifei Wang Jin Fu |
| author_facet | Xuemei Zhang Hao Tang Senlin Mao Bing Li Yinglian Zhou Hui Yue Duo Wang Yifei Wang Jin Fu |
| author_sort | Xuemei Zhang |
| collection | DOAJ |
| description | Abstract Background Ischaemic stroke has become the main cause of death and severe neurological disorders, for which effective restorative treatments are currently limited. While stem cell transplantation offers therapeutic potential through neural regeneration, this approach is associated with the challenges of limited applicable sources. Hair follicle stem cells (HFSCs) are multipotential cells that can differentiate into ectodermal and mesodermal lineages and proliferate for long periods. The therapeutic potentials of HFSCs have not been investigated in ischaemic stroke models, and therefore, in this study, we aimed to determine whether they could survive and migrate to ischaemic areas after a stroke attack. Methods A rat model of middle cerebral artery ischaemia/reperfusion was established and intravenously administered HFSCs. The potential of HFSCs to migrate and differentiate into neuron-like cells as well as their ability to reduce the infarct size was evaluated. Rat brain tissue samples were collected 2 weeks after cell transplantation and analysed via TTC staining, immunofluorescence and immunohistochemistry methods. The data were statistically analysed and presented as the means ± standard deviations. Results Intravenously administrated rat HFSCs were able to migrate to the penumbra where they expressed neuron-specific markers, reduced the infarct volume and promoted neurological recovery. Conclusion HFSC transplantation has therapeutic potential for ischaemic stroke and is, therefore, worthy of further investigation toward possible clinical development for treating stroke patients. |
| first_indexed | 2024-12-12T22:07:23Z |
| format | Article |
| id | doaj.art-1d47bbfddfc844c4a724796c228d7cd5 |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-12T22:07:23Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-1d47bbfddfc844c4a724796c228d7cd52022-12-22T00:10:21ZengBMCStem Cell Research & Therapy1757-65122020-09-0111111210.1186/s13287-020-01927-wTransplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusionXuemei Zhang0Hao Tang1Senlin Mao2Bing Li3Yinglian Zhou4Hui Yue5Duo Wang6Yifei Wang7Jin Fu8Department of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityDepartment of Neurology, The Second Affiliated Hospital of Harbin Medical UniversityAbstract Background Ischaemic stroke has become the main cause of death and severe neurological disorders, for which effective restorative treatments are currently limited. While stem cell transplantation offers therapeutic potential through neural regeneration, this approach is associated with the challenges of limited applicable sources. Hair follicle stem cells (HFSCs) are multipotential cells that can differentiate into ectodermal and mesodermal lineages and proliferate for long periods. The therapeutic potentials of HFSCs have not been investigated in ischaemic stroke models, and therefore, in this study, we aimed to determine whether they could survive and migrate to ischaemic areas after a stroke attack. Methods A rat model of middle cerebral artery ischaemia/reperfusion was established and intravenously administered HFSCs. The potential of HFSCs to migrate and differentiate into neuron-like cells as well as their ability to reduce the infarct size was evaluated. Rat brain tissue samples were collected 2 weeks after cell transplantation and analysed via TTC staining, immunofluorescence and immunohistochemistry methods. The data were statistically analysed and presented as the means ± standard deviations. Results Intravenously administrated rat HFSCs were able to migrate to the penumbra where they expressed neuron-specific markers, reduced the infarct volume and promoted neurological recovery. Conclusion HFSC transplantation has therapeutic potential for ischaemic stroke and is, therefore, worthy of further investigation toward possible clinical development for treating stroke patients.http://link.springer.com/article/10.1186/s13287-020-01927-wHair follicle stem cellsIschaemia/reperfusionCell transplantationHomingDifferentiation |
| spellingShingle | Xuemei Zhang Hao Tang Senlin Mao Bing Li Yinglian Zhou Hui Yue Duo Wang Yifei Wang Jin Fu Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusion Stem Cell Research & Therapy Hair follicle stem cells Ischaemia/reperfusion Cell transplantation Homing Differentiation |
| title | Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusion |
| title_full | Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusion |
| title_fullStr | Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusion |
| title_full_unstemmed | Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusion |
| title_short | Transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia/reperfusion |
| title_sort | transplanted hair follicle stem cells migrate to the penumbra and express neural markers in a rat model of cerebral ischaemia reperfusion |
| topic | Hair follicle stem cells Ischaemia/reperfusion Cell transplantation Homing Differentiation |
| url | http://link.springer.com/article/10.1186/s13287-020-01927-w |
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