Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomics
Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever and, in some cases, chronic carriage after resolution of acute disease. This study examined sequential isolates of S. Typhi from a single host with persistent asymptomatic infection. These isolates, along with another S. Typhi isolate...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10446203/?tool=EBI |
| _version_ | 1797736799456460800 |
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| author | Eby M. Sim Qinning Wang Peter Howard Rady Kim Ling Lim Kirsty Hope Vitali Sintchenko |
| author_facet | Eby M. Sim Qinning Wang Peter Howard Rady Kim Ling Lim Kirsty Hope Vitali Sintchenko |
| author_sort | Eby M. Sim |
| collection | DOAJ |
| description | Salmonella enterica serovar Typhi (S. Typhi) causes typhoid fever and, in some cases, chronic carriage after resolution of acute disease. This study examined sequential isolates of S. Typhi from a single host with persistent asymptomatic infection. These isolates, along with another S. Typhi isolate recovered from a household contact with typhoid fever, were subjected to whole genome sequencing and analysis. In addition, direct sequencing of the bile fluid from the host with persistent infection was also performed. Comparative analysis of isolates revealed three sub-populations of S. Typhi with distinct genetic patterns. Metagenomic sequencing recognised only two of the three sub-populations within the bile fluid. The detection and investigation of insertion sequences IS10R and associated deletions complemented analysis of single nucleotide polymorphisms. These findings improve our understanding of within-host dynamics of S. Typhi in cases of persistent infection and inform epidemiological investigations of transmission events associated with chronic carriers. |
| first_indexed | 2024-03-12T13:19:10Z |
| format | Article |
| id | doaj.art-1d482634bc0e4ebe9ac7f36063d78612 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-12T13:19:10Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1d482634bc0e4ebe9ac7f36063d786122023-08-26T05:31:37ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-01188Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomicsEby M. SimQinning WangPeter HowardRady KimLing LimKirsty HopeVitali SintchenkoSalmonella enterica serovar Typhi (S. Typhi) causes typhoid fever and, in some cases, chronic carriage after resolution of acute disease. This study examined sequential isolates of S. Typhi from a single host with persistent asymptomatic infection. These isolates, along with another S. Typhi isolate recovered from a household contact with typhoid fever, were subjected to whole genome sequencing and analysis. In addition, direct sequencing of the bile fluid from the host with persistent infection was also performed. Comparative analysis of isolates revealed three sub-populations of S. Typhi with distinct genetic patterns. Metagenomic sequencing recognised only two of the three sub-populations within the bile fluid. The detection and investigation of insertion sequences IS10R and associated deletions complemented analysis of single nucleotide polymorphisms. These findings improve our understanding of within-host dynamics of S. Typhi in cases of persistent infection and inform epidemiological investigations of transmission events associated with chronic carriers.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10446203/?tool=EBI |
| spellingShingle | Eby M. Sim Qinning Wang Peter Howard Rady Kim Ling Lim Kirsty Hope Vitali Sintchenko Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomics PLoS ONE |
| title | Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomics |
| title_full | Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomics |
| title_fullStr | Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomics |
| title_full_unstemmed | Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomics |
| title_short | Persistent Salmonella enterica serovar Typhi sub-populations within host interrogated by whole genome sequencing and metagenomics |
| title_sort | persistent salmonella enterica serovar typhi sub populations within host interrogated by whole genome sequencing and metagenomics |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10446203/?tool=EBI |
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