A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301D
Genetic and phenotypic mapping of an approximately 145-kb DraI fragment of Pseudomonas syringae pv. syringae strain B301D determined that the syringomycin (syr) and syringopeptin (syp) gene clusters are localized to this fragment. The syr and syp gene clusters encompass approximately 55 kb and appro...
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2001-12-01
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| Series: | Molecular Plant-Microbe Interactions |
| Subjects: | |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.12.1426 |
| _version_ | 1818647204981637120 |
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| author | Brenda K. Scholz-Schroeder Jonathan D. Soule Shi-En Lu Ingeborg Grgurina Dennis C. Gross |
| author_facet | Brenda K. Scholz-Schroeder Jonathan D. Soule Shi-En Lu Ingeborg Grgurina Dennis C. Gross |
| author_sort | Brenda K. Scholz-Schroeder |
| collection | DOAJ |
| description | Genetic and phenotypic mapping of an approximately 145-kb DraI fragment of Pseudomonas syringae pv. syringae strain B301D determined that the syringomycin (syr) and syringopeptin (syp) gene clusters are localized to this fragment. The syr and syp gene clusters encompass approximately 55 kb and approximately 80 kb, respectively. Both phytotoxins are synthesized by a thiotemplate mechanism of biosynthesis, requiring large multienzymatic proteins called peptide synthetases. Genes encoding peptide synthetases were identified within the syr and syp gene clusters, accounting for 90% of the DraI fragment. In addition, genes encoding regulatory and secretion proteins were localized to the DraI fragment. In particular, the salA gene, encoding a regulatory element responsible for syringomycin production and lesion formation in P. syringae pv. syringae strain B728a, was localized to the syr gene cluster. A putative ATP-binding cassette (ABC) transporter homolog was determined to be physically located in the syp gene cluster, but phenotypically affects production of both phytotoxins. Preliminary size estimates of the syr and syp gene clusters indicate that they represent two of the largest nonribosomal peptide synthetase gene clusters. Together, the syr and syp gene clusters encompass approximately 135 kb of DNA and may represent a genomic island in P. syringae pv. syringae that contributes to virulence in plant hosts. |
| first_indexed | 2024-12-17T00:58:49Z |
| format | Article |
| id | doaj.art-1d4909a1f74c4bb494947b868a45be64 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-12-17T00:58:49Z |
| publishDate | 2001-12-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-1d4909a1f74c4bb494947b868a45be642022-12-21T22:09:31ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062001-12-0114121426143510.1094/MPMI.2001.14.12.1426A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301DBrenda K. Scholz-SchroederJonathan D. SouleShi-En LuIngeborg GrgurinaDennis C. GrossGenetic and phenotypic mapping of an approximately 145-kb DraI fragment of Pseudomonas syringae pv. syringae strain B301D determined that the syringomycin (syr) and syringopeptin (syp) gene clusters are localized to this fragment. The syr and syp gene clusters encompass approximately 55 kb and approximately 80 kb, respectively. Both phytotoxins are synthesized by a thiotemplate mechanism of biosynthesis, requiring large multienzymatic proteins called peptide synthetases. Genes encoding peptide synthetases were identified within the syr and syp gene clusters, accounting for 90% of the DraI fragment. In addition, genes encoding regulatory and secretion proteins were localized to the DraI fragment. In particular, the salA gene, encoding a regulatory element responsible for syringomycin production and lesion formation in P. syringae pv. syringae strain B728a, was localized to the syr gene cluster. A putative ATP-binding cassette (ABC) transporter homolog was determined to be physically located in the syp gene cluster, but phenotypically affects production of both phytotoxins. Preliminary size estimates of the syr and syp gene clusters indicate that they represent two of the largest nonribosomal peptide synthetase gene clusters. Together, the syr and syp gene clusters encompass approximately 135 kb of DNA and may represent a genomic island in P. syringae pv. syringae that contributes to virulence in plant hosts.https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.12.1426lipopeptide toxinnecrosis-inducing phytotoxinvirulence determinant |
| spellingShingle | Brenda K. Scholz-Schroeder Jonathan D. Soule Shi-En Lu Ingeborg Grgurina Dennis C. Gross A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301D Molecular Plant-Microbe Interactions lipopeptide toxin necrosis-inducing phytotoxin virulence determinant |
| title | A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301D |
| title_full | A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301D |
| title_fullStr | A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301D |
| title_full_unstemmed | A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301D |
| title_short | A Physical Map of the Syringomycin and Syringopeptin Gene Clusters Localized to an Approximately 145-kb DNA Region of Pseudomonas syringae pv. syringae Strain B301D |
| title_sort | physical map of the syringomycin and syringopeptin gene clusters localized to an approximately 145 kb dna region of pseudomonas syringae pv syringae strain b301d |
| topic | lipopeptide toxin necrosis-inducing phytotoxin virulence determinant |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI.2001.14.12.1426 |
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