Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in Mice
Lead (Pb) exposure induces severe nephrotoxic effects in humans and animals. Herein, we compare the effects of two chelating agents, salinomycin and deferiprone, on Pb-induced renal alterations in mice and in the homeostasis of essential elements. Adult male mice (<i>Institute of Cancer Resear...
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2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/8/4368 |
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| author | Yordanka Gluhcheva Irena Pashkunova-Martic Martin Schaier Ivelin Vladov Silviya Stoykova Emilia Petrova Ekaterina Pavlova Peter Dorkov Thomas H. Helbich Bernhard Keppler Juliana Ivanova |
| author_facet | Yordanka Gluhcheva Irena Pashkunova-Martic Martin Schaier Ivelin Vladov Silviya Stoykova Emilia Petrova Ekaterina Pavlova Peter Dorkov Thomas H. Helbich Bernhard Keppler Juliana Ivanova |
| author_sort | Yordanka Gluhcheva |
| collection | DOAJ |
| description | Lead (Pb) exposure induces severe nephrotoxic effects in humans and animals. Herein, we compare the effects of two chelating agents, salinomycin and deferiprone, on Pb-induced renal alterations in mice and in the homeostasis of essential elements. Adult male mice (<i>Institute of Cancer Research</i> (<i>ICR</i>)) were randomized into four groups: control (Ctrl)—untreated mice administered distilled water for 28 days; Pb-exposed group (Pb)—mice administered orally an average daily dose of 80 mg/kg body weight (BW) lead (II) nitrate (Pb(NO<sub>3</sub>)<sub>2</sub>) during the first two weeks of the experimental protocol followed by the administration of distilled water for another two weeks; salinomycin-treated (Pb + Sal) group—Pb-exposed mice, administered an average daily dose of 16 mg/kg BW salinomycin for two weeks; deferiprone-treated (Pb + Def) group—Pb-exposed mice, administered an average daily dose of 20 mg/kg BW deferiprone for 14 days. The exposure of mice to Pb induced significant accumulation of the toxic metal in the kidneys and elicited inflammation with leukocyte infiltrations near the glomerulus. Biochemical analysis of the sera revealed that Pb significantly altered the renal function markers. Pb-induced renal toxicity was accompanied by a significant decrease in the endogenous renal concentrations of phosphorous (P), calcium (Ca), copper (Cu) and selenium (Se). In contrast to deferiprone, salinomycin significantly improved renal morphology in Pb-treated mice and decreased the Pb content by 13.62% compared to the Pb-exposed group. There was also a mild decrease in the renal endogenous concentration of magnesium (Mg) and elevation of the renal concentration of iron (Fe) in the salinomycin-treated group compared to controls. Overall, the results demonstrated that salinomycin is a more effective chelating agent for the treatment of Pb-induced alterations in renal morphology compared to deferiprone. |
| first_indexed | 2024-03-09T13:31:52Z |
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| id | doaj.art-1d4cdbc812984de78ae9e2334863b865 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T13:31:52Z |
| publishDate | 2022-04-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1d4cdbc812984de78ae9e2334863b8652023-11-30T21:16:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238436810.3390/ijms23084368Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in MiceYordanka Gluhcheva0Irena Pashkunova-Martic1Martin Schaier2Ivelin Vladov3Silviya Stoykova4Emilia Petrova5Ekaterina Pavlova6Peter Dorkov7Thomas H. Helbich8Bernhard Keppler9Juliana Ivanova10Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bl. 25, 1113 Sofia, BulgariaDepartment of Biomedical Imaging and Image-Guided Therapy, Division of Molecular and Structural Preclinical Imaging, Medical University of Vienna and General Hospital of Vienna, 18–20 Waehringer Guertel, 1090 Vienna, AustriaInstitute of Analytical Chemistry, University of Vienna, 38 Waehringer Strasse, 1090 Vienna, AustriaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bl. 25, 1113 Sofia, BulgariaFaculty of Chemistry and Pharmacy, Sofia University “St. Kliment Ohridski”, 1 J. Bourchier Blvd., 1164 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bl. 25, 1113 Sofia, BulgariaInstitute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Acad. Georgi Bonchev Street, Bl. 25, 1113 Sofia, BulgariaChemistry Department, Research and Development, BIOVET JSC, 39 Peter Rakov Street, 4550 Peshtera, BulgariaDepartment of Biomedical Imaging and Image-Guided Therapy, Division of Molecular and Structural Preclinical Imaging, Medical University of Vienna and General Hospital of Vienna, 18–20 Waehringer Guertel, 1090 Vienna, AustriaInstitute of Inorganic Chemistry, University of Vienna, 42 Waehringer Strasse, 1090 Vienna, AustriaFaculty of Medicine, Sofia University “St. Kliment Ohridski”, 1 Kozjak Street, 1407 Sofia, BulgariaLead (Pb) exposure induces severe nephrotoxic effects in humans and animals. Herein, we compare the effects of two chelating agents, salinomycin and deferiprone, on Pb-induced renal alterations in mice and in the homeostasis of essential elements. Adult male mice (<i>Institute of Cancer Research</i> (<i>ICR</i>)) were randomized into four groups: control (Ctrl)—untreated mice administered distilled water for 28 days; Pb-exposed group (Pb)—mice administered orally an average daily dose of 80 mg/kg body weight (BW) lead (II) nitrate (Pb(NO<sub>3</sub>)<sub>2</sub>) during the first two weeks of the experimental protocol followed by the administration of distilled water for another two weeks; salinomycin-treated (Pb + Sal) group—Pb-exposed mice, administered an average daily dose of 16 mg/kg BW salinomycin for two weeks; deferiprone-treated (Pb + Def) group—Pb-exposed mice, administered an average daily dose of 20 mg/kg BW deferiprone for 14 days. The exposure of mice to Pb induced significant accumulation of the toxic metal in the kidneys and elicited inflammation with leukocyte infiltrations near the glomerulus. Biochemical analysis of the sera revealed that Pb significantly altered the renal function markers. Pb-induced renal toxicity was accompanied by a significant decrease in the endogenous renal concentrations of phosphorous (P), calcium (Ca), copper (Cu) and selenium (Se). In contrast to deferiprone, salinomycin significantly improved renal morphology in Pb-treated mice and decreased the Pb content by 13.62% compared to the Pb-exposed group. There was also a mild decrease in the renal endogenous concentration of magnesium (Mg) and elevation of the renal concentration of iron (Fe) in the salinomycin-treated group compared to controls. Overall, the results demonstrated that salinomycin is a more effective chelating agent for the treatment of Pb-induced alterations in renal morphology compared to deferiprone.https://www.mdpi.com/1422-0067/23/8/4368deferipronesalinomycinleadessential elementskidneys |
| spellingShingle | Yordanka Gluhcheva Irena Pashkunova-Martic Martin Schaier Ivelin Vladov Silviya Stoykova Emilia Petrova Ekaterina Pavlova Peter Dorkov Thomas H. Helbich Bernhard Keppler Juliana Ivanova Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in Mice International Journal of Molecular Sciences deferiprone salinomycin lead essential elements kidneys |
| title | Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in Mice |
| title_full | Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in Mice |
| title_fullStr | Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in Mice |
| title_full_unstemmed | Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in Mice |
| title_short | Comparative Effects of Deferiprone and Salinomycin on Lead-Induced Disturbance in the Homeostasis of Intrarenal Essential Elements in Mice |
| title_sort | comparative effects of deferiprone and salinomycin on lead induced disturbance in the homeostasis of intrarenal essential elements in mice |
| topic | deferiprone salinomycin lead essential elements kidneys |
| url | https://www.mdpi.com/1422-0067/23/8/4368 |
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