Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical Production
Chiral compounds are valuable industrial products and intermediates, and the production of chemicals with high enantiopurity is one of the major objects in asymmetric catalysis. Compared with traditional chemical synthesis, enzymatic synthesis can produce chiral molecules under sustainable condition...
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MDPI AG
2023-01-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/13/2/288 |
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| author | Mingzhe Qiu Sheng Dong Qiu Cui Yingang Feng Jinsong Xuan |
| author_facet | Mingzhe Qiu Sheng Dong Qiu Cui Yingang Feng Jinsong Xuan |
| author_sort | Mingzhe Qiu |
| collection | DOAJ |
| description | Chiral compounds are valuable industrial products and intermediates, and the production of chemicals with high enantiopurity is one of the major objects in asymmetric catalysis. Compared with traditional chemical synthesis, enzymatic synthesis can produce chiral molecules under sustainable conditions which are much greener, more economical, and more environmentally friendly. The superfamily of α/β hydrolases includes a lot of diverse enzymes showing excellent chemo-, regio-, and enantio-selectivity in asymmetric synthesis and many of them are biocatalysts in industry. This review outlines the current knowledge of the structures and reaction mechanism of α/β hydrolases and summarizes the screening and protein engineering efforts to develop biocatalysts for chiral chemicals production in recent years. Other strategies such as whole-cell catalysis and protein immobilization to improve the performance of α/β hydrolases are also discussed. The progress in biocatalyst development based on α/β hydrolases will promote the biosynthesis of chiral compounds, thus contributing to the green and sustainable development of the chemical and pharmaceutical industry. |
| first_indexed | 2024-03-11T09:01:39Z |
| format | Article |
| id | doaj.art-1d4e57e212464026868db2d22ebc4391 |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-11T09:01:39Z |
| publishDate | 2023-01-01 |
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| series | Catalysts |
| spelling | doaj.art-1d4e57e212464026868db2d22ebc43912023-11-16T19:41:01ZengMDPI AGCatalysts2073-43442023-01-0113228810.3390/catal13020288Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical ProductionMingzhe Qiu0Sheng Dong1Qiu Cui2Yingang Feng3Jinsong Xuan4Department of Bioscience and Bioengineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Beijing 100083, ChinaCAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, ChinaCAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, ChinaCAS Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, 189 Songling Road, Qingdao 266101, ChinaDepartment of Bioscience and Bioengineering, School of Chemistry and Biological Engineering, University of Science and Technology Beijing, 30 Xueyuan Road, Beijing 100083, ChinaChiral compounds are valuable industrial products and intermediates, and the production of chemicals with high enantiopurity is one of the major objects in asymmetric catalysis. Compared with traditional chemical synthesis, enzymatic synthesis can produce chiral molecules under sustainable conditions which are much greener, more economical, and more environmentally friendly. The superfamily of α/β hydrolases includes a lot of diverse enzymes showing excellent chemo-, regio-, and enantio-selectivity in asymmetric synthesis and many of them are biocatalysts in industry. This review outlines the current knowledge of the structures and reaction mechanism of α/β hydrolases and summarizes the screening and protein engineering efforts to develop biocatalysts for chiral chemicals production in recent years. Other strategies such as whole-cell catalysis and protein immobilization to improve the performance of α/β hydrolases are also discussed. The progress in biocatalyst development based on α/β hydrolases will promote the biosynthesis of chiral compounds, thus contributing to the green and sustainable development of the chemical and pharmaceutical industry.https://www.mdpi.com/2073-4344/13/2/288protein engineeringchiral compoundα/β hydrolasecatalytic mechanismenantioselectivity |
| spellingShingle | Mingzhe Qiu Sheng Dong Qiu Cui Yingang Feng Jinsong Xuan Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical Production Catalysts protein engineering chiral compound α/β hydrolase catalytic mechanism enantioselectivity |
| title | Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical Production |
| title_full | Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical Production |
| title_fullStr | Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical Production |
| title_full_unstemmed | Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical Production |
| title_short | Recent Progress in the Mechanism and Engineering of α/β Hydrolases for Chiral Chemical Production |
| title_sort | recent progress in the mechanism and engineering of α β hydrolases for chiral chemical production |
| topic | protein engineering chiral compound α/β hydrolase catalytic mechanism enantioselectivity |
| url | https://www.mdpi.com/2073-4344/13/2/288 |
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