In-vitro Inhibition of Biofilm Formation in Candida albicans and Candida tropicalis by Heat Stable Compounds in Culture Filtrate of Aspergillus flavus
Background: Invasive candidiasis, caused mostly by Candida albicans and C. tropicalis is one of the most common causes of bloodstream infection with a substantial attributable mortality. This disease is associated with formation of structured, multilayered microbial communities known as biofilms...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2013-10-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/3460/17-%206020_E(C)_F(T)_PF1(Re_Formatting)_PFA(H)_PF2(Bo_PUH).pdf |
| Summary: | Background: Invasive candidiasis, caused mostly by Candida
albicans and C. tropicalis is one of the most common causes of
bloodstream infection with a substantial attributable mortality. This
disease is associated with formation of structured, multilayered
microbial communities known as biofilms over indwelling
devices. Treatment is rendered difficult owing to factors like poor
drug penetration through biofilms and high cost of the available
antifungal drugs. Hence there is imminent need of developing
low-cost natural compounds inhibiting Candidal biofilm formation
in vitro. Organohalgen compounds derived from crude culture
filtrate of Aspergillus flavus have been documented to impair in
vitro Candidal survival.
Aim: We aimed to detect the effect of preheated and unheated
crude culture filtrate of Aspergillus flavus on biofilm formation of
Candida albicans and C. tropicalis in vitro.
Setting and Designs: Ours was a laboratory-based observational
study with clinical isolates of the microorganisms selected
randomly.
Material and Methods: In this study, we showed for the first
time by microtitre plate method that heat stable compounds
which were present in preheated and unheated culture filtrates of
Aspergillus flavus inhibited biofilm formation of Candida albicans
and C. tropicalis and also lipase activities of these pathogens,
and filtrate was non-toxic on human cell line as checked
microscopically.
Statistical Analysis used: Z-test of significance was used to
calculate significant difference between Candidal biofilm formation
in normal liquid medium and culture filtrate, respectively.
Results and Conclusion: Heat stable compounds present in
culture filtrate of Aspergillus flavus inhibit biofilm formation of
Candida albicans and C. tropicalis and also in-vitro lipase activity
of these pathogens and could pave the way for development of
low-cost alternatives to treat invasive candidiasis. |
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| ISSN: | 2249-782X 0973-709X |