NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor.
In plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell p...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2020-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0241627 |
| _version_ | 1818195598835187712 |
|---|---|
| author | Thomas E Wilkop Minmin Wang Angelo Heringer Jaideep Singh Florence Zakharov Viswanathan V Krishnan Georgia Drakakaki |
| author_facet | Thomas E Wilkop Minmin Wang Angelo Heringer Jaideep Singh Florence Zakharov Viswanathan V Krishnan Georgia Drakakaki |
| author_sort | Thomas E Wilkop |
| collection | DOAJ |
| description | In plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell plate, arresting late-stage cytokinesis in arabidopsis. Endosidin-7 has emerged as a very valuable tool for dissecting this essential plant process. To gain insights regarding its mode of action and the effects of cytokinesis inhibition on the overall plant response, we investigated the effect of endosidin-7 through a nuclear magnetic resonance spectroscopy (NMR) metabolomics approach. In this case study, metabolomics profiles of arabidopsis leaf and root tissues were analyzed at different growth stages and endosidin-7 exposure levels. The results show leaf and root-specific metabolic profile changes and the effects of endosidin-7 treatment on these metabolomes. Statistical analyses indicated that the effect of endosidin-7 treatment was more significant than the developmental impact. The endosidin-7 induced metabolic profiles suggest compensations for cytokinesis inhibition in central metabolism pathways. This study further shows that long-term treatment of endosidin-7 profoundly changes, likely via alteration of hormonal regulation, the primary metabolism of arabidopsis seedlings. Hormonal pathway-changes are likely reflecting the plant's responses, compensating for the arrested cell division, which in turn are leading to global metabolite modulation. The presented NMR spectral data are made available through the Metabolomics Workbench, providing a reference resource for the scientific community. |
| first_indexed | 2024-12-12T01:20:44Z |
| format | Article |
| id | doaj.art-1d50aab9128d4ab48001b98f12eda203 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T01:20:44Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1d50aab9128d4ab48001b98f12eda2032022-12-22T00:43:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011511e024162710.1371/journal.pone.0241627NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor.Thomas E WilkopMinmin WangAngelo HeringerJaideep SinghFlorence ZakharovViswanathan V KrishnanGeorgia DrakakakiIn plant cytokinesis, de novo formation of a cell plate evolving into the new cell wall partitions the cytoplasm of the dividing cell. In our earlier chemical genomics studies, we identified and characterized the small molecule endosidin-7, that specifically inhibits callose deposition at the cell plate, arresting late-stage cytokinesis in arabidopsis. Endosidin-7 has emerged as a very valuable tool for dissecting this essential plant process. To gain insights regarding its mode of action and the effects of cytokinesis inhibition on the overall plant response, we investigated the effect of endosidin-7 through a nuclear magnetic resonance spectroscopy (NMR) metabolomics approach. In this case study, metabolomics profiles of arabidopsis leaf and root tissues were analyzed at different growth stages and endosidin-7 exposure levels. The results show leaf and root-specific metabolic profile changes and the effects of endosidin-7 treatment on these metabolomes. Statistical analyses indicated that the effect of endosidin-7 treatment was more significant than the developmental impact. The endosidin-7 induced metabolic profiles suggest compensations for cytokinesis inhibition in central metabolism pathways. This study further shows that long-term treatment of endosidin-7 profoundly changes, likely via alteration of hormonal regulation, the primary metabolism of arabidopsis seedlings. Hormonal pathway-changes are likely reflecting the plant's responses, compensating for the arrested cell division, which in turn are leading to global metabolite modulation. The presented NMR spectral data are made available through the Metabolomics Workbench, providing a reference resource for the scientific community.https://doi.org/10.1371/journal.pone.0241627 |
| spellingShingle | Thomas E Wilkop Minmin Wang Angelo Heringer Jaideep Singh Florence Zakharov Viswanathan V Krishnan Georgia Drakakaki NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor. PLoS ONE |
| title | NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor. |
| title_full | NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor. |
| title_fullStr | NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor. |
| title_full_unstemmed | NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor. |
| title_short | NMR spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor. |
| title_sort | nmr spectroscopy analysis reveals differential metabolic responses in arabidopsis roots and leaves treated with a cytokinesis inhibitor |
| url | https://doi.org/10.1371/journal.pone.0241627 |
| work_keys_str_mv | AT thomasewilkop nmrspectroscopyanalysisrevealsdifferentialmetabolicresponsesinarabidopsisrootsandleavestreatedwithacytokinesisinhibitor AT minminwang nmrspectroscopyanalysisrevealsdifferentialmetabolicresponsesinarabidopsisrootsandleavestreatedwithacytokinesisinhibitor AT angeloheringer nmrspectroscopyanalysisrevealsdifferentialmetabolicresponsesinarabidopsisrootsandleavestreatedwithacytokinesisinhibitor AT jaideepsingh nmrspectroscopyanalysisrevealsdifferentialmetabolicresponsesinarabidopsisrootsandleavestreatedwithacytokinesisinhibitor AT florencezakharov nmrspectroscopyanalysisrevealsdifferentialmetabolicresponsesinarabidopsisrootsandleavestreatedwithacytokinesisinhibitor AT viswanathanvkrishnan nmrspectroscopyanalysisrevealsdifferentialmetabolicresponsesinarabidopsisrootsandleavestreatedwithacytokinesisinhibitor AT georgiadrakakaki nmrspectroscopyanalysisrevealsdifferentialmetabolicresponsesinarabidopsisrootsandleavestreatedwithacytokinesisinhibitor |