Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosa
Phaeocystis globosa blooms can have negative effects on higher trophic levels in the marine ecosystem and consequently influence human activities. Strain KA22, identified as the bacterium Hahella, was isolated from coastal surface water and used to control P. globosa growth. A methanol extract from...
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2016-04-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00602/full |
| _version_ | 1828823622858309632 |
|---|---|
| author | Huajun eZhang Huajun eZhang Yun ePeng Su eZhang Guanjing eCai Yi eLi Xujun eYang Ke eYang Zhangran eChen Jun eZhang Hui eWang Wei eZheng Tianling eZheng |
| author_facet | Huajun eZhang Huajun eZhang Yun ePeng Su eZhang Guanjing eCai Yi eLi Xujun eYang Ke eYang Zhangran eChen Jun eZhang Hui eWang Wei eZheng Tianling eZheng |
| author_sort | Huajun eZhang |
| collection | DOAJ |
| description | Phaeocystis globosa blooms can have negative effects on higher trophic levels in the marine ecosystem and consequently influence human activities. Strain KA22, identified as the bacterium Hahella, was isolated from coastal surface water and used to control P. globosa growth. A methanol extract from the bacteral cells showed strong algicidal activity. After purification, the compound showed a similar structure to prodigiosin when identified with Q-Exactive Orbitrap MS and nuclear magnetic resonance spectra. The compound showed algicidal activity against P. globosa with a 50% Lethal Dose (LD50) of 2.24 μg/mL. The prodigiosin was stable under heat and acid environment, and it could be degraded under alkaline environment and natural light condition. The growth rates of strain KA22 was fast in 2216E medium and the content of prodigiosin in this medium was more than 70 μg/mL after 16 h incubation. The compound showed particularly strong algicidal activity against Prorocentrum donghaiense, P. globosa and Heterosigma akashiwo, but having little effect on three other phytoplankton species tested. The results of our research could increase our knowledge on harmful algal bloom control compound and lead to further study on the mechanisms of the lysis effect on harmful algae. |
| first_indexed | 2024-12-12T13:38:48Z |
| format | Article |
| id | doaj.art-1d50bc77e02c47e39c28410b6db91d63 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T13:38:48Z |
| publishDate | 2016-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-1d50bc77e02c47e39c28410b6db91d632022-12-22T00:22:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2016-04-01710.3389/fmicb.2016.00602179338Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosaHuajun eZhang0Huajun eZhang1Yun ePeng2Su eZhang3Guanjing eCai4Yi eLi5Xujun eYang6Ke eYang7Zhangran eChen8Jun eZhang9Hui eWang10Wei eZheng11Tianling eZheng12Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaSchool of Marine Sciences, Ningbo University, Ningbo 315211, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaDepartment of biology, college of science, Shantou university, 515063, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaKey Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, School of Life Sciences, Xiamen University, Xiamen, ChinaPhaeocystis globosa blooms can have negative effects on higher trophic levels in the marine ecosystem and consequently influence human activities. Strain KA22, identified as the bacterium Hahella, was isolated from coastal surface water and used to control P. globosa growth. A methanol extract from the bacteral cells showed strong algicidal activity. After purification, the compound showed a similar structure to prodigiosin when identified with Q-Exactive Orbitrap MS and nuclear magnetic resonance spectra. The compound showed algicidal activity against P. globosa with a 50% Lethal Dose (LD50) of 2.24 μg/mL. The prodigiosin was stable under heat and acid environment, and it could be degraded under alkaline environment and natural light condition. The growth rates of strain KA22 was fast in 2216E medium and the content of prodigiosin in this medium was more than 70 μg/mL after 16 h incubation. The compound showed particularly strong algicidal activity against Prorocentrum donghaiense, P. globosa and Heterosigma akashiwo, but having little effect on three other phytoplankton species tested. The results of our research could increase our knowledge on harmful algal bloom control compound and lead to further study on the mechanisms of the lysis effect on harmful algae.http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00602/fullProdigiosinred tideAlgicidal bacteriumPhaeocystis globosaanti-algal |
| spellingShingle | Huajun eZhang Huajun eZhang Yun ePeng Su eZhang Guanjing eCai Yi eLi Xujun eYang Ke eYang Zhangran eChen Jun eZhang Hui eWang Wei eZheng Tianling eZheng Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosa Frontiers in Microbiology Prodigiosin red tide Algicidal bacterium Phaeocystis globosa anti-algal |
| title | Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosa |
| title_full | Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosa |
| title_fullStr | Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosa |
| title_full_unstemmed | Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosa |
| title_short | Effectiveness of an anti-algal compound in eliminating an aquatic unicellular harmful algal Phaeocystis globosa |
| title_sort | effectiveness of an anti algal compound in eliminating an aquatic unicellular harmful algal phaeocystis globosa |
| topic | Prodigiosin red tide Algicidal bacterium Phaeocystis globosa anti-algal |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2016.00602/full |
| work_keys_str_mv | AT huajunezhang effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT huajunezhang effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT yunepeng effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT suezhang effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT guanjingecai effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT yieli effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT xujuneyang effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT keeyang effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT zhangranechen effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT junezhang effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT huiewang effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT weiezheng effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa AT tianlingezheng effectivenessofanantialgalcompoundineliminatinganaquaticunicellularharmfulalgalphaeocystisglobosa |