Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms
Weichao Zhao,1,2,* Huqin Yang,1,* Yong Huang,3,* Xin Fan,4 Zhaohui Tong1 1Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Department...
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| Aineistotyyppi: | Artikkeli |
| Kieli: | English |
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Dove Medical Press
2024-02-01
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| Sarja: | Infection and Drug Resistance |
| Aiheet: | |
| Linkit: | https://www.dovepress.com/genomic-sequencing-of-clinical-cupriavidus-gilardii-isolates-revealed--peer-reviewed-fulltext-article-IDR |
| _version_ | 1827347578452180992 |
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| author | Zhao W Yang H Huang Y Fan X Tong Z |
| author_facet | Zhao W Yang H Huang Y Fan X Tong Z |
| author_sort | Zhao W |
| collection | DOAJ |
| description | Weichao Zhao,1,2,* Huqin Yang,1,* Yong Huang,3,* Xin Fan,4 Zhaohui Tong1 1Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Department of Respiratory Medicine, Strategic Support Force Medical Center, Beijing, People’s Republic of China; 3State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of China; 4Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhaohui Tong; Xin Fan, NO. 8 Gong Ti South Road, Beijing, 10020, People’s Republic of China, Tel +86 1085231297 ; +86 13521438062, Email tongzhaohuicy@sina.com; fanxin123356@163.comPurpose: Cupriavidus gilardii is an emerging multidrug-resistant pathogen found in many environments and few clinical samples. The clinical infectiousness, pathogenicity, and resistance mechanisms of C. gilardii are still unclear due to the lack of clinical and sequencing data. We need to obtain insight into the clinical characteristics, virulence, and resistance mechanisms of C. gilardii.Patients and Methods: We isolated five C. gilardii isolates from hospitalized patients and carried out assay, culture and genome sequencing. We analyzed the genomic features of clinical C. gilardii isolates and took insight into their clinical characteristics, virulence, and resistance mechanisms.Results: These isolates were resistant to meropenem, gentamicin, and other antimicrobials due to intrinsic resistance genes. Furthermore, the sequencing results revealed the widespread presence of the MCR-5.1 gene in C. gilardii. The virulence magnitude of C. gilardii is closely correlated with the number of virulence factors they carry. Some C. gilardii strains can acquire resistance to levofloxacin through gyrA gene mutation during treatment. The diverse antimicrobial resistance mechanisms challenge the treatment of C. gilardii infections.Conclusion: We present the genomic characteristics of clinically isolated C. gilardii to improve (i) our understanding of this pathogen and (ii) treatment options.Keywords: Cupriavidus gilardii, clinical characteristics, antimicrobial resistance, MCR-5.1, gyrA |
| first_indexed | 2024-03-07T23:53:42Z |
| format | Article |
| id | doaj.art-1d51a727d27c41ee9a1eb139c5e43dd8 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-03-07T23:53:42Z |
| publishDate | 2024-02-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-1d51a727d27c41ee9a1eb139c5e43dd82024-02-18T16:42:54ZengDove Medical PressInfection and Drug Resistance1178-69732024-02-01Volume 1765566490518Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance MechanismsZhao WYang HHuang YFan XTong ZWeichao Zhao,1,2,* Huqin Yang,1,* Yong Huang,3,* Xin Fan,4 Zhaohui Tong1 1Department of Respiratory and Critical Care Medicine, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China; 2Department of Respiratory Medicine, Strategic Support Force Medical Center, Beijing, People’s Republic of China; 3State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, Beijing, People’s Republic of China; 4Department of Infectious Diseases and Clinical Microbiology, Beijing Institute of Respiratory Medicine, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zhaohui Tong; Xin Fan, NO. 8 Gong Ti South Road, Beijing, 10020, People’s Republic of China, Tel +86 1085231297 ; +86 13521438062, Email tongzhaohuicy@sina.com; fanxin123356@163.comPurpose: Cupriavidus gilardii is an emerging multidrug-resistant pathogen found in many environments and few clinical samples. The clinical infectiousness, pathogenicity, and resistance mechanisms of C. gilardii are still unclear due to the lack of clinical and sequencing data. We need to obtain insight into the clinical characteristics, virulence, and resistance mechanisms of C. gilardii.Patients and Methods: We isolated five C. gilardii isolates from hospitalized patients and carried out assay, culture and genome sequencing. We analyzed the genomic features of clinical C. gilardii isolates and took insight into their clinical characteristics, virulence, and resistance mechanisms.Results: These isolates were resistant to meropenem, gentamicin, and other antimicrobials due to intrinsic resistance genes. Furthermore, the sequencing results revealed the widespread presence of the MCR-5.1 gene in C. gilardii. The virulence magnitude of C. gilardii is closely correlated with the number of virulence factors they carry. Some C. gilardii strains can acquire resistance to levofloxacin through gyrA gene mutation during treatment. The diverse antimicrobial resistance mechanisms challenge the treatment of C. gilardii infections.Conclusion: We present the genomic characteristics of clinically isolated C. gilardii to improve (i) our understanding of this pathogen and (ii) treatment options.Keywords: Cupriavidus gilardii, clinical characteristics, antimicrobial resistance, MCR-5.1, gyrAhttps://www.dovepress.com/genomic-sequencing-of-clinical-cupriavidus-gilardii-isolates-revealed--peer-reviewed-fulltext-article-IDRcupriavidus gilardiiclinical characteristicsantimicrobial resistancemcr-5.1gyra. |
| spellingShingle | Zhao W Yang H Huang Y Fan X Tong Z Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms Infection and Drug Resistance cupriavidus gilardii clinical characteristics antimicrobial resistance mcr-5.1 gyra. |
| title | Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms |
| title_full | Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms |
| title_fullStr | Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms |
| title_full_unstemmed | Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms |
| title_short | Genomic Sequencing of Clinical Cupriavidus gilardii Isolates Revealed Their Diverse Antimicrobial Resistance Mechanisms |
| title_sort | genomic sequencing of clinical cupriavidus gilardii isolates revealed their diverse antimicrobial resistance mechanisms |
| topic | cupriavidus gilardii clinical characteristics antimicrobial resistance mcr-5.1 gyra. |
| url | https://www.dovepress.com/genomic-sequencing-of-clinical-cupriavidus-gilardii-isolates-revealed--peer-reviewed-fulltext-article-IDR |
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