Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus
In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating th...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Vaccines |
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| Online Access: | https://www.mdpi.com/2076-393X/12/2/143 |
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| author | Nadezhda B. Rudometova Anastasia A. Fando Lyubov A. Kisakova Denis N. Kisakov Mariya B. Borgoyakova Victoria R. Litvinova Vladimir A. Yakovlev Elena V. Tigeeva Danil I. Vahitov Sergey V. Sharabrin Dmitriy N. Shcherbakov Veronika I. Evseenko Ksenia I. Ivanova Andrei S. Gudymo Tatiana N. Ilyicheva Vasiliy Yu. Marchenko Alexander A. Ilyichev Andrey P. Rudometov Larisa I. Karpenko |
| author_facet | Nadezhda B. Rudometova Anastasia A. Fando Lyubov A. Kisakova Denis N. Kisakov Mariya B. Borgoyakova Victoria R. Litvinova Vladimir A. Yakovlev Elena V. Tigeeva Danil I. Vahitov Sergey V. Sharabrin Dmitriy N. Shcherbakov Veronika I. Evseenko Ksenia I. Ivanova Andrei S. Gudymo Tatiana N. Ilyicheva Vasiliy Yu. Marchenko Alexander A. Ilyichev Andrey P. Rudometov Larisa I. Karpenko |
| author_sort | Nadezhda B. Rudometova |
| collection | DOAJ |
| description | In this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus. The results demonstrated that both immunogens induced a specific antibody response as determined by ELISA. Virus neutralization assay revealed that sera of immunized animals were able to neutralize A/turkey/Stavropol/320-01/2020 (H5N8) influenza virus—the average neutralizing titer was 2560. Immunization with both recombinant HA/H5 hemagglutinin and inactivated virus gave 100% protection against lethal H5N8 virus challenge. This study shows that recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against influenza A (H5N8) virus with suitable immunogenicity and protective efficacy. |
| first_indexed | 2024-03-07T22:10:48Z |
| format | Article |
| id | doaj.art-1d51e53906da4e168b48bd8fd815c67c |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-07T22:10:48Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-1d51e53906da4e168b48bd8fd815c67c2024-02-23T15:36:58ZengMDPI AGVaccines2076-393X2024-01-0112214310.3390/vaccines12020143Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) VirusNadezhda B. Rudometova0Anastasia A. Fando1Lyubov A. Kisakova2Denis N. Kisakov3Mariya B. Borgoyakova4Victoria R. Litvinova5Vladimir A. Yakovlev6Elena V. Tigeeva7Danil I. Vahitov8Sergey V. Sharabrin9Dmitriy N. Shcherbakov10Veronika I. Evseenko11Ksenia I. Ivanova12Andrei S. Gudymo13Tatiana N. Ilyicheva14Vasiliy Yu. Marchenko15Alexander A. Ilyichev16Andrey P. Rudometov17Larisa I. Karpenko18Federal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaInstitute of Solid State Chemistry and Mechanochemistry, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaFederal Budgetary Research Institution State Research Center of Virology and Biotechnology «Vector», Rospotrebnadzor, Koltsovo 630559, Novosibirsk Region, RussiaIn this study, we characterized recombinant hemagglutinin (HA) of influenza A (H5N8) virus produced in Chinese hamster ovary cells (CHO-K1s). Immunochemical analysis showed that the recombinant hemagglutinin was recognized by the serum of ferrets infected with influenza A (H5N8) virus, indicating that its antigenic properties were retained. Two groups of Balb/c mice were immunized with intramuscular injection of recombinant hemagglutinin or propiolactone inactivated A/Astrakhan/3212/2020 (H5N8) influenza virus. The results demonstrated that both immunogens induced a specific antibody response as determined by ELISA. Virus neutralization assay revealed that sera of immunized animals were able to neutralize A/turkey/Stavropol/320-01/2020 (H5N8) influenza virus—the average neutralizing titer was 2560. Immunization with both recombinant HA/H5 hemagglutinin and inactivated virus gave 100% protection against lethal H5N8 virus challenge. This study shows that recombinant HA (H5N8) protein may be a useful antigen candidate for developing subunit vaccines against influenza A (H5N8) virus with suitable immunogenicity and protective efficacy.https://www.mdpi.com/2076-393X/12/2/143influenza A (H5N8) virusrecombinant hemagglutininCHO-K1immunogenicityprotection |
| spellingShingle | Nadezhda B. Rudometova Anastasia A. Fando Lyubov A. Kisakova Denis N. Kisakov Mariya B. Borgoyakova Victoria R. Litvinova Vladimir A. Yakovlev Elena V. Tigeeva Danil I. Vahitov Sergey V. Sharabrin Dmitriy N. Shcherbakov Veronika I. Evseenko Ksenia I. Ivanova Andrei S. Gudymo Tatiana N. Ilyicheva Vasiliy Yu. Marchenko Alexander A. Ilyichev Andrey P. Rudometov Larisa I. Karpenko Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus Vaccines influenza A (H5N8) virus recombinant hemagglutinin CHO-K1 immunogenicity protection |
| title | Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus |
| title_full | Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus |
| title_fullStr | Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus |
| title_full_unstemmed | Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus |
| title_short | Immunogenic and Protective Properties of Recombinant Hemagglutinin of Influenza A (H5N8) Virus |
| title_sort | immunogenic and protective properties of recombinant hemagglutinin of influenza a h5n8 virus |
| topic | influenza A (H5N8) virus recombinant hemagglutinin CHO-K1 immunogenicity protection |
| url | https://www.mdpi.com/2076-393X/12/2/143 |
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