Generation of the human erythroblast-derived iPSC line UBTi001-A
We performed reprogramming of human erythroblasts derived from CD34+ hematopoietic stem / progenitor cells of a healthy donor. CD34+ cells were differentiated in-vitro into a pure population of CD36+ erythroblasts and nucleofected with four episomal plasmids expressing SOX2, OCT3/4, KLF4, LIN28, L-M...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-10-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506122002598 |
| _version_ | 1828321960218591232 |
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| author | Afrim Avdili Lisa Rohrhofer Martina Auer Claudia Bernecker Peter Schlenke Isabel Dorn |
| author_facet | Afrim Avdili Lisa Rohrhofer Martina Auer Claudia Bernecker Peter Schlenke Isabel Dorn |
| author_sort | Afrim Avdili |
| collection | DOAJ |
| description | We performed reprogramming of human erythroblasts derived from CD34+ hematopoietic stem / progenitor cells of a healthy donor. CD34+ cells were differentiated in-vitro into a pure population of CD36+ erythroblasts and nucleofected with four episomal plasmids expressing SOX2, OCT3/4, KLF4, LIN28, L-MYC and TP53-shRNA. The established iPSC line showed normal karyotype. Pluripotency was confirmed by expression of pluripotency markers and in-vitro differentiation into tissues of all three germ layers. The UBTi001-A iPSC line might provide an attractive source for developmental research on human hematopoiesis and erythropoiesis. |
| first_indexed | 2024-04-13T18:33:51Z |
| format | Article |
| id | doaj.art-1d520636f1cc449b85e5eb1a3cc9b609 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-13T18:33:51Z |
| publishDate | 2022-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-1d520636f1cc449b85e5eb1a3cc9b6092022-12-22T02:34:57ZengElsevierStem Cell Research1873-50612022-10-0164102910Generation of the human erythroblast-derived iPSC line UBTi001-AAfrim Avdili0Lisa Rohrhofer1Martina Auer2Claudia Bernecker3Peter Schlenke4Isabel Dorn5Department of Blood Group Serology and Transfusion Medicine, Medical University of Graz, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Graz, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Graz, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Graz, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Graz, AustriaDepartment of Blood Group Serology and Transfusion Medicine, Medical University of Graz, AustriaWe performed reprogramming of human erythroblasts derived from CD34+ hematopoietic stem / progenitor cells of a healthy donor. CD34+ cells were differentiated in-vitro into a pure population of CD36+ erythroblasts and nucleofected with four episomal plasmids expressing SOX2, OCT3/4, KLF4, LIN28, L-MYC and TP53-shRNA. The established iPSC line showed normal karyotype. Pluripotency was confirmed by expression of pluripotency markers and in-vitro differentiation into tissues of all three germ layers. The UBTi001-A iPSC line might provide an attractive source for developmental research on human hematopoiesis and erythropoiesis.http://www.sciencedirect.com/science/article/pii/S1873506122002598 |
| spellingShingle | Afrim Avdili Lisa Rohrhofer Martina Auer Claudia Bernecker Peter Schlenke Isabel Dorn Generation of the human erythroblast-derived iPSC line UBTi001-A Stem Cell Research |
| title | Generation of the human erythroblast-derived iPSC line UBTi001-A |
| title_full | Generation of the human erythroblast-derived iPSC line UBTi001-A |
| title_fullStr | Generation of the human erythroblast-derived iPSC line UBTi001-A |
| title_full_unstemmed | Generation of the human erythroblast-derived iPSC line UBTi001-A |
| title_short | Generation of the human erythroblast-derived iPSC line UBTi001-A |
| title_sort | generation of the human erythroblast derived ipsc line ubti001 a |
| url | http://www.sciencedirect.com/science/article/pii/S1873506122002598 |
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