Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani
Fusarium solani is a pathogenic fungus that causes significant harm, leading to crop yield reduction, fruit quality reduction, postharvest decay, and other diseases. This study used potato glycoside alkaloids (PGA) as inhibitors to investigate their effects on the mitochondrial structure and tricarb...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1390269/full |
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| author | Chongqing Zhang Wei Chen Bin Wang Yupeng Wang Nan Li Ruiyun Li Yuke Yan Yuyan Sun Jing He Jing He |
| author_facet | Chongqing Zhang Wei Chen Bin Wang Yupeng Wang Nan Li Ruiyun Li Yuke Yan Yuyan Sun Jing He Jing He |
| author_sort | Chongqing Zhang |
| collection | DOAJ |
| description | Fusarium solani is a pathogenic fungus that causes significant harm, leading to crop yield reduction, fruit quality reduction, postharvest decay, and other diseases. This study used potato glycoside alkaloids (PGA) as inhibitors to investigate their effects on the mitochondrial structure and tricarboxylic acid (TCA) cycle pathway of F. solani. The results showed that PGA could inhibit the colony growth of F. solani (54.49%), resulting in the disappearance of the mitochondrial membrane and the loss of contents. PGA significantly decreased the activities of aconitase (ACO), isocitrate dehydrogenase (IDH), α-ketoglutarate dehydrogenase (α-KGDH), succinate dehydrogenase (SDH), fumarase (FH), malate dehydrogenase (MDH), succinyl-CoA synthetase (SCS), and increased the activity of citrate synthase (CS) in F. solani. After PGA treatment, the contents of acetyl coenzyme A (CoA), citric acid (CA), malic acid (L-MA), and α-ketoglutaric acid (α-KG) in F. solani were significantly decreased. The contents of isocitric acid (ICA), succinyl coenzyme A (S-CoA), succinic acid (SA), fumaric acid (FA), and oxaloacetic acid (OA) were significantly increased. Transcriptomic analysis showed that PGA could significantly affect the expression levels of 19 genes related to TCA cycle in F. solani. RT-qPCR results showed that the expression levels of ACO, IDH, α-KGDH, and MDH-related genes were significantly down-regulated, and the expression levels of SDH and FH-related genes were significantly up-regulated, which was consistent with the results of transcriptomics. In summary, PGA can achieve antifungal effects by reducing the tricarboxylic acid cycle’s flow and regulating key genes’ expression levels. This study reveals the antifungal mechanism of PGA from the perspective of TCA cycle, and provides a theoretical basis for the development and application of PGA as a biopesticide. |
| first_indexed | 2024-04-24T09:44:03Z |
| format | Article |
| id | doaj.art-1d534b7121274134bffe329e61115b11 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-24T09:44:03Z |
| publishDate | 2024-04-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-1d534b7121274134bffe329e61115b112024-04-15T04:15:13ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2024-04-011510.3389/fmicb.2024.13902691390269Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solaniChongqing Zhang0Wei Chen1Bin Wang2Yupeng Wang3Nan Li4Ruiyun Li5Yuke Yan6Yuyan Sun7Jing He8Jing He9College of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaCollege of Forestry, Gansu Agricultural University, Lanzhou, ChinaWolfberry Harmless Cultivation Engineering Research Center of Gansu Province, Lanzhou, ChinaFusarium solani is a pathogenic fungus that causes significant harm, leading to crop yield reduction, fruit quality reduction, postharvest decay, and other diseases. This study used potato glycoside alkaloids (PGA) as inhibitors to investigate their effects on the mitochondrial structure and tricarboxylic acid (TCA) cycle pathway of F. solani. The results showed that PGA could inhibit the colony growth of F. solani (54.49%), resulting in the disappearance of the mitochondrial membrane and the loss of contents. PGA significantly decreased the activities of aconitase (ACO), isocitrate dehydrogenase (IDH), α-ketoglutarate dehydrogenase (α-KGDH), succinate dehydrogenase (SDH), fumarase (FH), malate dehydrogenase (MDH), succinyl-CoA synthetase (SCS), and increased the activity of citrate synthase (CS) in F. solani. After PGA treatment, the contents of acetyl coenzyme A (CoA), citric acid (CA), malic acid (L-MA), and α-ketoglutaric acid (α-KG) in F. solani were significantly decreased. The contents of isocitric acid (ICA), succinyl coenzyme A (S-CoA), succinic acid (SA), fumaric acid (FA), and oxaloacetic acid (OA) were significantly increased. Transcriptomic analysis showed that PGA could significantly affect the expression levels of 19 genes related to TCA cycle in F. solani. RT-qPCR results showed that the expression levels of ACO, IDH, α-KGDH, and MDH-related genes were significantly down-regulated, and the expression levels of SDH and FH-related genes were significantly up-regulated, which was consistent with the results of transcriptomics. In summary, PGA can achieve antifungal effects by reducing the tricarboxylic acid cycle’s flow and regulating key genes’ expression levels. This study reveals the antifungal mechanism of PGA from the perspective of TCA cycle, and provides a theoretical basis for the development and application of PGA as a biopesticide.https://www.frontiersin.org/articles/10.3389/fmicb.2024.1390269/fullpotato glycoside alkaloidsFusarium solanitricarboxylic acid cyclemitochondrion structuregene expression |
| spellingShingle | Chongqing Zhang Wei Chen Bin Wang Yupeng Wang Nan Li Ruiyun Li Yuke Yan Yuyan Sun Jing He Jing He Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani Frontiers in Microbiology potato glycoside alkaloids Fusarium solani tricarboxylic acid cycle mitochondrion structure gene expression |
| title | Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani |
| title_full | Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani |
| title_fullStr | Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani |
| title_full_unstemmed | Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani |
| title_short | Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani |
| title_sort | potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of fusarium solani |
| topic | potato glycoside alkaloids Fusarium solani tricarboxylic acid cycle mitochondrion structure gene expression |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2024.1390269/full |
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