Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina
The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in th...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-09-01
|
| Series: | Toxicon: X |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590171020300254 |
| _version_ | 1818402732990529536 |
|---|---|
| author | Luciano S. Fusco Emidio B. Neto Aleff F. Francisco Jorge Alfonso Andreimar Soares Daniel C. Pimenta Laura C. Leiva |
| author_facet | Luciano S. Fusco Emidio B. Neto Aleff F. Francisco Jorge Alfonso Andreimar Soares Daniel C. Pimenta Laura C. Leiva |
| author_sort | Luciano S. Fusco |
| collection | DOAJ |
| description | The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy. |
| first_indexed | 2024-12-14T08:13:03Z |
| format | Article |
| id | doaj.art-1d5578b738114b819280e6b9d7793469 |
| institution | Directory Open Access Journal |
| issn | 2590-1710 |
| language | English |
| last_indexed | 2024-12-14T08:13:03Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Toxicon: X |
| spelling | doaj.art-1d5578b738114b819280e6b9d77934692022-12-21T23:10:01ZengElsevierToxicon: X2590-17102020-09-017100047Fast venomic analysis of Crotalus durissus terrificus from northeastern ArgentinaLuciano S. Fusco0Emidio B. Neto1Aleff F. Francisco2Jorge Alfonso3Andreimar Soares4Daniel C. Pimenta5Laura C. Leiva6Laboratorio de Investigación en Proteínas (LabInPro), Instituto de Química Básica y aplicada del Nordeste Argentino (IQUIBA-NEA, CONICET), Universidad Nacional del Nordeste (UNNE), Corrientes, Argentina; Corresponding author.Laboratorio de Bioquímica y Biofísica, Instituto Butantan, São Paulo, SP, BrazilDepartamento de Física e Biofísica, Instituto de Biociências, São Paulo State University, UNESP, Botucatu, SP, BrazilCentro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz (FIOCRUZ), Fiocruz Rondônia, Porto Velho-RO e Universidade Federal de Rondônia (UNIR), Porto Velho, RO, Brazil; Centro Para el Desarrollo de la Investigación Científica (CEDIC), Asunción, ParaguayCentro de Estudos de Biomoléculas Aplicadas à Saúde (CEBio), Fundação Oswaldo Cruz (FIOCRUZ), Fiocruz Rondônia, Porto Velho-RO e Universidade Federal de Rondônia (UNIR), Porto Velho, RO, Brazil; Centro Universitário São Lucas (UniSL), Porto Velho, RO, BrazilLaboratorio de Bioquímica y Biofísica, Instituto Butantan, São Paulo, SP, BrazilLaboratorio de Investigación en Proteínas (LabInPro), Instituto de Química Básica y aplicada del Nordeste Argentino (IQUIBA-NEA, CONICET), Universidad Nacional del Nordeste (UNNE), Corrientes, Argentina; Corresponding author.The complete knowledge of the toxins that make up venoms is the base for the treatment of snake accidents victims and the selection of specimens for the preparation of venom pools for antivenom production. In this work, we used a fast and direct venomics approach to identify the toxin families in the C.d. terrificus venom, a Southern American Neotropical rattlesnake. The RP-HPLC separation profile of pooled venom from adult specimens followed by mass spectrometry analysis revealed that C.d. terrificus’ venom proteome is composed of 12 protein families, which are unevenly distributed in the venom, e.g., there are few major proteins in the venom's composition phospholipase A2, serine proteinase, crotamine and L-amino acid oxidase. At the same time, the proteome analysis revealed a small set of proteins with low quantity (less than 1.5%), both enzymes (metaloprotease, phospholipase B and 5′-nucleotidase) and proteins (Bradykinin potentiating and C-type natriuretic peptides, C-type lectin convulxin and nerve growth factor). To sum up, this research is the first venomic report of C.d.terrificus venom from Argentina. This proved to be crotamine positive venom that has a lower metalloprotease content than C.d. terrificus venoms from other regions. This information could be used in the discovery of future pharmacological agents or targets in antivenom therapy.http://www.sciencedirect.com/science/article/pii/S2590171020300254 |
| spellingShingle | Luciano S. Fusco Emidio B. Neto Aleff F. Francisco Jorge Alfonso Andreimar Soares Daniel C. Pimenta Laura C. Leiva Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina Toxicon: X |
| title | Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina |
| title_full | Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina |
| title_fullStr | Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina |
| title_full_unstemmed | Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina |
| title_short | Fast venomic analysis of Crotalus durissus terrificus from northeastern Argentina |
| title_sort | fast venomic analysis of crotalus durissus terrificus from northeastern argentina |
| url | http://www.sciencedirect.com/science/article/pii/S2590171020300254 |
| work_keys_str_mv | AT lucianosfusco fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina AT emidiobneto fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina AT aleffffrancisco fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina AT jorgealfonso fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina AT andreimarsoares fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina AT danielcpimenta fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina AT lauracleiva fastvenomicanalysisofcrotalusdurissusterrificusfromnortheasternargentina |