Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in Stool
DNA amplification of exfoliated cells in stool repre sents an inexpensive and rapid test, but has only 50% to 60% sensitivity. A new quantitative method, calle( fluorescence long DNA, was developed and validate( in our laboratory on stool obtained from 86 patient., with primary colorectal cancer and...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2004-09-01
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| Series: | Neoplasia: An International Journal for Oncology Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1476558604800319 |
| _version_ | 1818387361932771328 |
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| author | Daniele Calistri Claudia Rengucci Arturo Lattuneddu Gianfranco Francioni Anna Maria Polifemo Oriana Nanni Luca Saragoni Franco Monti Alberto Ravaioli Wainer Zoli Dino Amadori |
| author_facet | Daniele Calistri Claudia Rengucci Arturo Lattuneddu Gianfranco Francioni Anna Maria Polifemo Oriana Nanni Luca Saragoni Franco Monti Alberto Ravaioli Wainer Zoli Dino Amadori |
| author_sort | Daniele Calistri |
| collection | DOAJ |
| description | DNA amplification of exfoliated cells in stool repre sents an inexpensive and rapid test, but has only 50% to 60% sensitivity. A new quantitative method, calle( fluorescence long DNA, was developed and validate( in our laboratory on stool obtained from 86 patient., with primary colorectal cancer and from 62 health individuals. It consists of the amplification of stoo DNA with fluorescence primers and the quantification of the amplification using a standard curve. Results are arbitrarily expressed in nanograms. The potential of thi new method compared to the conventional approact was analyzed in a subgroup of 94 individuals (51 patients and 38 healthy volunteers). In the presen series, DNA amplification analysis showed a specific ity of 97% and a sensitivity of only 50%. Conversely fluorescence DNA evaluation, using the best cutoff o 25 ng, showed a sensitivity of about 76% and a spec ificity of 93%. Similar sensitivity was observed regard less of Dukes stage, tumor location, and size, thu., also permitting the detection of early-stage tumors The present study seems to indicate that quantitative fluorescence DNA determination in stool successfully identifies colorectal cancer patients with a sensitivity comparable, if not superior, to that of multiple gene analysis but at a lower cost and in a shorter time. |
| first_indexed | 2024-12-14T04:08:44Z |
| format | Article |
| id | doaj.art-1d564c6dc0514a3bb68076af2d6e0586 |
| institution | Directory Open Access Journal |
| issn | 1476-5586 1522-8002 |
| language | English |
| last_indexed | 2024-12-14T04:08:44Z |
| publishDate | 2004-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Neoplasia: An International Journal for Oncology Research |
| spelling | doaj.art-1d564c6dc0514a3bb68076af2d6e05862022-12-21T23:17:45ZengElsevierNeoplasia: An International Journal for Oncology Research1476-55861522-80022004-09-016553654010.1593/neo.04190Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in StoolDaniele Calistri0Claudia Rengucci1Arturo Lattuneddu2Gianfranco Francioni3Anna Maria Polifemo4Oriana Nanni5Luca Saragoni6Franco Monti7Alberto Ravaioli8Wainer Zoli9Dino Amadori10Division of Oncology and Diagnostics, Pierantoni Hospital, Forli, ItalyDivision of Oncology and Diagnostics, Pierantoni Hospital, Forli, ItalyDepartment of Surgery I, Morgagni Hospital, Forlì, ItalyDepartment of General Surgery, Infermi Hospital, Rimini, ItalyGastroenterology Unit, Morgagni Hospital, Forli, ItalyDivision of Oncology and Diagnostics, Pierantoni Hospital, Forli, ItalyDivision of Oncology and Diagnostics, Pierantoni Hospital, Forli, ItalyDepartment of Oncology, Infermi Hospital, Rimini, ItalyDepartment of Oncology, Infermi Hospital, Rimini, ItalyDivision of Oncology and Diagnostics, Pierantoni Hospital, Forli, ItalyDivision of Oncology and Diagnostics, Pierantoni Hospital, Forli, ItalyDNA amplification of exfoliated cells in stool repre sents an inexpensive and rapid test, but has only 50% to 60% sensitivity. A new quantitative method, calle( fluorescence long DNA, was developed and validate( in our laboratory on stool obtained from 86 patient., with primary colorectal cancer and from 62 health individuals. It consists of the amplification of stoo DNA with fluorescence primers and the quantification of the amplification using a standard curve. Results are arbitrarily expressed in nanograms. The potential of thi new method compared to the conventional approact was analyzed in a subgroup of 94 individuals (51 patients and 38 healthy volunteers). In the presen series, DNA amplification analysis showed a specific ity of 97% and a sensitivity of only 50%. Conversely fluorescence DNA evaluation, using the best cutoff o 25 ng, showed a sensitivity of about 76% and a spec ificity of 93%. Similar sensitivity was observed regard less of Dukes stage, tumor location, and size, thu., also permitting the detection of early-stage tumors The present study seems to indicate that quantitative fluorescence DNA determination in stool successfully identifies colorectal cancer patients with a sensitivity comparable, if not superior, to that of multiple gene analysis but at a lower cost and in a shorter time.http://www.sciencedirect.com/science/article/pii/S1476558604800319Colorectal cancerstoolDNA amplificationdiagnosismolecular markers |
| spellingShingle | Daniele Calistri Claudia Rengucci Arturo Lattuneddu Gianfranco Francioni Anna Maria Polifemo Oriana Nanni Luca Saragoni Franco Monti Alberto Ravaioli Wainer Zoli Dino Amadori Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in Stool Neoplasia: An International Journal for Oncology Research Colorectal cancer stool DNA amplification diagnosis molecular markers |
| title | Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in Stool |
| title_full | Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in Stool |
| title_fullStr | Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in Stool |
| title_full_unstemmed | Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in Stool |
| title_short | Detection of Colorectal Cancer by a Quantitative Fluorescence Determination of DNA Amplification in Stool |
| title_sort | detection of colorectal cancer by a quantitative fluorescence determination of dna amplification in stool |
| topic | Colorectal cancer stool DNA amplification diagnosis molecular markers |
| url | http://www.sciencedirect.com/science/article/pii/S1476558604800319 |
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