CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells

Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms....

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Main Authors: Xuelian Wang, Xiumin Huang, Xiuli Fang, Youzhong Zhang, Wanpeng Wang
Format: Article
Language:English
Published: Elsevier 2016-01-01
Series:Molecular Therapy: Nucleic Acids
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2162253117301154
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author Xuelian Wang
Xiumin Huang
Xiuli Fang
Youzhong Zhang
Wanpeng Wang
author_facet Xuelian Wang
Xiumin Huang
Xiuli Fang
Youzhong Zhang
Wanpeng Wang
author_sort Xuelian Wang
collection DOAJ
description Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clearly detailing a 20-nt targeting sequence inside its directed RNA. We discuss the most recent advancements in the molecular basis of the type II CRISPR/Cas system and encapsulate applications and elements affecting its use in human cells. We also propose possible applications covering its uses ranging from basic science to implementation in the clinic.
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spelling doaj.art-1d56589e26f24f299e36b1545af911a82022-12-21T23:55:46ZengElsevierMolecular Therapy: Nucleic Acids2162-25312016-01-015C10.1038/mtna.2016.95CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human CellsXuelian Wang0Xiumin Huang1Xiuli Fang2Youzhong Zhang3Wanpeng Wang4Department of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan, ChinaKey Laboratory of Marine Biogenetic Resources, the Third Institute of Oceanography, State of Oceanic Administration, Xiamen, ChinaTargeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clearly detailing a 20-nt targeting sequence inside its directed RNA. We discuss the most recent advancements in the molecular basis of the type II CRISPR/Cas system and encapsulate applications and elements affecting its use in human cells. We also propose possible applications covering its uses ranging from basic science to implementation in the clinic.http://www.sciencedirect.com/science/article/pii/S2162253117301154CRISPRcrRNACas9genome editinghuman cellsRGEN
spellingShingle Xuelian Wang
Xiumin Huang
Xiuli Fang
Youzhong Zhang
Wanpeng Wang
CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells
Molecular Therapy: Nucleic Acids
CRISPR
crRNA
Cas9
genome editing
human cells
RGEN
title CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells
title_full CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells
title_fullStr CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells
title_full_unstemmed CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells
title_short CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells
title_sort crispr cas9 system as a versatile tool for genome engineering in human cells
topic CRISPR
crRNA
Cas9
genome editing
human cells
RGEN
url http://www.sciencedirect.com/science/article/pii/S2162253117301154
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