CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells
Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms....
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Format: | Article |
Language: | English |
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Elsevier
2016-01-01
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Series: | Molecular Therapy: Nucleic Acids |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S2162253117301154 |
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author | Xuelian Wang Xiumin Huang Xiuli Fang Youzhong Zhang Wanpeng Wang |
author_facet | Xuelian Wang Xiumin Huang Xiuli Fang Youzhong Zhang Wanpeng Wang |
author_sort | Xuelian Wang |
collection | DOAJ |
description | Targeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clearly detailing a 20-nt targeting sequence inside its directed RNA. We discuss the most recent advancements in the molecular basis of the type II CRISPR/Cas system and encapsulate applications and elements affecting its use in human cells. We also propose possible applications covering its uses ranging from basic science to implementation in the clinic. |
first_indexed | 2024-12-13T07:07:44Z |
format | Article |
id | doaj.art-1d56589e26f24f299e36b1545af911a8 |
institution | Directory Open Access Journal |
issn | 2162-2531 |
language | English |
last_indexed | 2024-12-13T07:07:44Z |
publishDate | 2016-01-01 |
publisher | Elsevier |
record_format | Article |
series | Molecular Therapy: Nucleic Acids |
spelling | doaj.art-1d56589e26f24f299e36b1545af911a82022-12-21T23:55:46ZengElsevierMolecular Therapy: Nucleic Acids2162-25312016-01-015C10.1038/mtna.2016.95CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human CellsXuelian Wang0Xiumin Huang1Xiuli Fang2Youzhong Zhang3Wanpeng Wang4Department of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, Zhongshan Hospital of Xiamen University, Xiamen, ChinaDepartment of Gynecology and Obstetrics, Qilu Hospital of Shandong University, Jinan, ChinaKey Laboratory of Marine Biogenetic Resources, the Third Institute of Oceanography, State of Oceanic Administration, Xiamen, ChinaTargeted nucleases are influential instruments for intervening in genome revision with great accuracy. RNA-guided Cas9 nucleases produced from clustered regularly interspaced short palindromic repeats (CRISPR)-Cas systems have noticeably altered the means to modify the genomes of distinct organisms. They can be notably used to facilitate effective genome manipulation in eukaryotic cells by clearly detailing a 20-nt targeting sequence inside its directed RNA. We discuss the most recent advancements in the molecular basis of the type II CRISPR/Cas system and encapsulate applications and elements affecting its use in human cells. We also propose possible applications covering its uses ranging from basic science to implementation in the clinic.http://www.sciencedirect.com/science/article/pii/S2162253117301154CRISPRcrRNACas9genome editinghuman cellsRGEN |
spellingShingle | Xuelian Wang Xiumin Huang Xiuli Fang Youzhong Zhang Wanpeng Wang CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells Molecular Therapy: Nucleic Acids CRISPR crRNA Cas9 genome editing human cells RGEN |
title | CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells |
title_full | CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells |
title_fullStr | CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells |
title_full_unstemmed | CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells |
title_short | CRISPR-Cas9 System as a Versatile Tool for Genome Engineering in Human Cells |
title_sort | crispr cas9 system as a versatile tool for genome engineering in human cells |
topic | CRISPR crRNA Cas9 genome editing human cells RGEN |
url | http://www.sciencedirect.com/science/article/pii/S2162253117301154 |
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