Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species

Pathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treat...

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Main Authors: Anvitha Hansoge, Sudheesh S Nair, M V Chinnu, M.K. Narayanan, K.D. John Martin
Format: Article
Language:English
Published: Director of Academics and Research, Kerala Veterinary and Animal Sciences University 2021-06-01
Series:Journal of Veterinary and Animal Sciences
Subjects:
Online Access:https://www.jvas.in/public_html/upload/article_file/article_file_qvr8z4.pdf?t=qvr8z4
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author Anvitha Hansoge
Sudheesh S Nair
M V Chinnu
M.K. Narayanan
K.D. John Martin
author_facet Anvitha Hansoge
Sudheesh S Nair
M V Chinnu
M.K. Narayanan
K.D. John Martin
author_sort Anvitha Hansoge
collection DOAJ
description Pathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treatment and prevention protocols. Four cases of chronic obstructive urolithiasis in male goats presented to University Veterinary Hospital were surgically managed by tube cystotomy. The calculi extracted from the bladder were subjected to Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) to identify the composition of the calculi. The infrared wavelength bandwidths of H-O-H stretching vibrations, H-O-H bending modes of vibrations of water molecules, N-H symmetric stretching vibrations, N-H bending vibration and N-H asymmetric bending vibration in NH4+ unit’s ionic phosphate were compared with the available reference spectrum of wavelength and were found to be identical and comparable with the standard infrared wavelength of struvite calculi.
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spelling doaj.art-1d58d5b2218d46acb3e82a77662739612022-12-21T23:13:58ZengDirector of Academics and Research, Kerala Veterinary and Animal Sciences UniversityJournal of Veterinary and Animal Sciences0971-07012582-06052021-06-0152212513010.51966/jvas.2021.52.2.125-130Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine speciesAnvitha Hansoge0Sudheesh S Nair1https://orcid.org/0000-0002-1209-8741M V Chinnu2 M.K. Narayanan3K.D. John Martin4Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaPathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treatment and prevention protocols. Four cases of chronic obstructive urolithiasis in male goats presented to University Veterinary Hospital were surgically managed by tube cystotomy. The calculi extracted from the bladder were subjected to Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) to identify the composition of the calculi. The infrared wavelength bandwidths of H-O-H stretching vibrations, H-O-H bending modes of vibrations of water molecules, N-H symmetric stretching vibrations, N-H bending vibration and N-H asymmetric bending vibration in NH4+ unit’s ionic phosphate were compared with the available reference spectrum of wavelength and were found to be identical and comparable with the standard infrared wavelength of struvite calculi.https://www.jvas.in/public_html/upload/article_file/article_file_qvr8z4.pdf?t=qvr8z4fourier transform infrared spectrophotometerattenuated total reflectancechronic obstructive urolithiasisgoats
spellingShingle Anvitha Hansoge
Sudheesh S Nair
M V Chinnu
M.K. Narayanan
K.D. John Martin
Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
Journal of Veterinary and Animal Sciences
fourier transform infrared spectrophotometer
attenuated total reflectance
chronic obstructive urolithiasis
goats
title Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
title_full Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
title_fullStr Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
title_full_unstemmed Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
title_short Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
title_sort fourier transform infrared spectrophotometer with attenuated total reflectance ftir atr for analysis of uroliths in caprine species
topic fourier transform infrared spectrophotometer
attenuated total reflectance
chronic obstructive urolithiasis
goats
url https://www.jvas.in/public_html/upload/article_file/article_file_qvr8z4.pdf?t=qvr8z4
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