Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species
Pathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treat...
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Director of Academics and Research, Kerala Veterinary and Animal Sciences University
2021-06-01
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Online Access: | https://www.jvas.in/public_html/upload/article_file/article_file_qvr8z4.pdf?t=qvr8z4 |
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author | Anvitha Hansoge Sudheesh S Nair M V Chinnu M.K. Narayanan K.D. John Martin |
author_facet | Anvitha Hansoge Sudheesh S Nair M V Chinnu M.K. Narayanan K.D. John Martin |
author_sort | Anvitha Hansoge |
collection | DOAJ |
description | Pathophysiology of obstructive urolithiasis in goats are multifactorial involving
management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treatment and prevention protocols. Four cases of chronic obstructive urolithiasis in male goats presented to University Veterinary Hospital were surgically managed by tube
cystotomy. The calculi extracted from the bladder were subjected to Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) to identify the composition of the calculi. The infrared wavelength bandwidths of H-O-H stretching vibrations, H-O-H bending modes of vibrations of water molecules, N-H symmetric stretching vibrations, N-H bending vibration and N-H asymmetric bending vibration in NH4+ unit’s ionic phosphate were compared with the
available reference spectrum of wavelength and were found to be identical and comparable with the standard infrared wavelength of struvite calculi. |
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issn | 0971-0701 2582-0605 |
language | English |
last_indexed | 2024-12-14T06:18:02Z |
publishDate | 2021-06-01 |
publisher | Director of Academics and Research, Kerala Veterinary and Animal Sciences University |
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series | Journal of Veterinary and Animal Sciences |
spelling | doaj.art-1d58d5b2218d46acb3e82a77662739612022-12-21T23:13:58ZengDirector of Academics and Research, Kerala Veterinary and Animal Sciences UniversityJournal of Veterinary and Animal Sciences0971-07012582-06052021-06-0152212513010.51966/jvas.2021.52.2.125-130Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine speciesAnvitha Hansoge0Sudheesh S Nair1https://orcid.org/0000-0002-1209-8741M V Chinnu2 M.K. Narayanan3K.D. John Martin4Department of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Biochemistry, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaDepartment of Veterinary Surgery and Radiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala Veterinary and Animal Sciences University, IndiaPathophysiology of obstructive urolithiasis in goats are multifactorial involving management, nutritional and hormonal factors. Analysis of the composition of a particular urolith would aid in understanding the aetiopathogenesis of the calculi which can facilitate establishment of appropriate treatment and prevention protocols. Four cases of chronic obstructive urolithiasis in male goats presented to University Veterinary Hospital were surgically managed by tube cystotomy. The calculi extracted from the bladder were subjected to Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) to identify the composition of the calculi. The infrared wavelength bandwidths of H-O-H stretching vibrations, H-O-H bending modes of vibrations of water molecules, N-H symmetric stretching vibrations, N-H bending vibration and N-H asymmetric bending vibration in NH4+ unit’s ionic phosphate were compared with the available reference spectrum of wavelength and were found to be identical and comparable with the standard infrared wavelength of struvite calculi.https://www.jvas.in/public_html/upload/article_file/article_file_qvr8z4.pdf?t=qvr8z4fourier transform infrared spectrophotometerattenuated total reflectancechronic obstructive urolithiasisgoats |
spellingShingle | Anvitha Hansoge Sudheesh S Nair M V Chinnu M.K. Narayanan K.D. John Martin Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species Journal of Veterinary and Animal Sciences fourier transform infrared spectrophotometer attenuated total reflectance chronic obstructive urolithiasis goats |
title | Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species |
title_full | Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species |
title_fullStr | Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species |
title_full_unstemmed | Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species |
title_short | Fourier transform infrared spectrophotometer with attenuated total reflectance (FTIR-ATR) for analysis of uroliths in caprine species |
title_sort | fourier transform infrared spectrophotometer with attenuated total reflectance ftir atr for analysis of uroliths in caprine species |
topic | fourier transform infrared spectrophotometer attenuated total reflectance chronic obstructive urolithiasis goats |
url | https://www.jvas.in/public_html/upload/article_file/article_file_qvr8z4.pdf?t=qvr8z4 |
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