Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles
Jiayu Zhang,1 Shiqing Ma,1 Zihao Liu,1 Hongjuan Geng,1 Xin Lu,1 Xi Zhang,1 Hongjie Li,1 Chenyuan Gao,2 Xu Zhang,1 Ping Gao1 1School of Dentistry, Hospital of Stomatology, Tianjin Medical University, Tianjin, 2Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Bei...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2017-12-01
|
| Series: | International Journal of Nanomedicine |
| Subjects: | |
| Online Access: | https://www.dovepress.com/guided-bone-regeneration-with-asymmetric-collagen-chitosan-membranes-c-peer-reviewed-article-IJN |
| _version_ | 1819200329927884800 |
|---|---|
| author | Zhang J Ma S Liu Z Geng H Lu X Zhang X Li H Gao C Zhang X Gao P |
| author_facet | Zhang J Ma S Liu Z Geng H Lu X Zhang X Li H Gao C Zhang X Gao P |
| author_sort | Zhang J |
| collection | DOAJ |
| description | Jiayu Zhang,1 Shiqing Ma,1 Zihao Liu,1 Hongjuan Geng,1 Xin Lu,1 Xi Zhang,1 Hongjie Li,1 Chenyuan Gao,2 Xu Zhang,1 Ping Gao1 1School of Dentistry, Hospital of Stomatology, Tianjin Medical University, Tianjin, 2Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, People’s Republic of China Introduction: Membranes allowing the sustained release of drugs that can achieve cell adhesion are very promising for guided bone regeneration. Previous studies have suggested that aspirin has the potential to promote bone regeneration. The purpose of this study was to prepare a local drug delivery system with aspirin-loaded chitosan nanoparticles (ACS) contained in an asymmetric collagen-chitosan membrane (CCM). Methods: In this study, the ACS were fabricated using different concentrations of aspirin (5 mg, 25 mg, 50 mg, and 75 mg). The drug release behavior of ACS was studied. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to examine the micromorphology of ACS and aspirin-loaded chitosan nanoparticles contained in chitosan-collagen membranes (ACS-CCM). In vitro bone mesenchymal stem cells (BMSCs) were cultured and critical-sized cranial defects on Sprague-Dawley rats were made to evaluate the effect of the ACS-CCM on bone regeneration.Results: Drug release behavior results of ACS showed that the nanoparticles fabricated in this study could successfully sustain the release of the drug. TEM showed the morphology of the nanoparticles. SEM images indicated that the asymmetric membrane comprised a loose collagen layer and a dense chitosan layer. In vitro studies showed that ACS-CCM could promote the proliferation of BMSCs, and that the degree of differentiated BMSCs seeded on CCMs containing 50 mg of ACS was higher than that of other membranes. Micro-computed tomography showed that 50 mg of ACS-CCM resulted in enhanced bone regeneration compared with the control group.Conclusion: This study shows that the ACS-CCM would allow the sustained release of aspirin and have further osteogenic potential. This membrane is a promising therapeutic approach to guiding bone regeneration. Keywords: aspirin, nanoparticle, drug release, membrane, guided bone regeneration |
| first_indexed | 2024-12-23T03:30:31Z |
| format | Article |
| id | doaj.art-1d5960495e134aef94f9f6ef2a0e3ab8 |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-23T03:30:31Z |
| publishDate | 2017-12-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-1d5960495e134aef94f9f6ef2a0e3ab82022-12-21T18:01:42ZengDove Medical PressInternational Journal of Nanomedicine1178-20132017-12-01Volume 128855886636036Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticlesZhang JMa SLiu ZGeng HLu XZhang XLi HGao CZhang XGao PJiayu Zhang,1 Shiqing Ma,1 Zihao Liu,1 Hongjuan Geng,1 Xin Lu,1 Xi Zhang,1 Hongjie Li,1 Chenyuan Gao,2 Xu Zhang,1 Ping Gao1 1School of Dentistry, Hospital of Stomatology, Tianjin Medical University, Tianjin, 2Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing, People’s Republic of China Introduction: Membranes allowing the sustained release of drugs that can achieve cell adhesion are very promising for guided bone regeneration. Previous studies have suggested that aspirin has the potential to promote bone regeneration. The purpose of this study was to prepare a local drug delivery system with aspirin-loaded chitosan nanoparticles (ACS) contained in an asymmetric collagen-chitosan membrane (CCM). Methods: In this study, the ACS were fabricated using different concentrations of aspirin (5 mg, 25 mg, 50 mg, and 75 mg). The drug release behavior of ACS was studied. Transmission electron microscopy (TEM) and scanning electron microscopy (SEM) were used to examine the micromorphology of ACS and aspirin-loaded chitosan nanoparticles contained in chitosan-collagen membranes (ACS-CCM). In vitro bone mesenchymal stem cells (BMSCs) were cultured and critical-sized cranial defects on Sprague-Dawley rats were made to evaluate the effect of the ACS-CCM on bone regeneration.Results: Drug release behavior results of ACS showed that the nanoparticles fabricated in this study could successfully sustain the release of the drug. TEM showed the morphology of the nanoparticles. SEM images indicated that the asymmetric membrane comprised a loose collagen layer and a dense chitosan layer. In vitro studies showed that ACS-CCM could promote the proliferation of BMSCs, and that the degree of differentiated BMSCs seeded on CCMs containing 50 mg of ACS was higher than that of other membranes. Micro-computed tomography showed that 50 mg of ACS-CCM resulted in enhanced bone regeneration compared with the control group.Conclusion: This study shows that the ACS-CCM would allow the sustained release of aspirin and have further osteogenic potential. This membrane is a promising therapeutic approach to guiding bone regeneration. Keywords: aspirin, nanoparticle, drug release, membrane, guided bone regenerationhttps://www.dovepress.com/guided-bone-regeneration-with-asymmetric-collagen-chitosan-membranes-c-peer-reviewed-article-IJNaspirinnanoparticledrug releasemembraneguided bone regeneration |
| spellingShingle | Zhang J Ma S Liu Z Geng H Lu X Zhang X Li H Gao C Zhang X Gao P Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles International Journal of Nanomedicine aspirin nanoparticle drug release membrane guided bone regeneration |
| title | Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles |
| title_full | Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles |
| title_fullStr | Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles |
| title_full_unstemmed | Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles |
| title_short | Guided bone regeneration with asymmetric collagen-chitosan membranes containing aspirin-loaded chitosan nanoparticles |
| title_sort | guided bone regeneration with asymmetric collagen chitosan membranes containing aspirin loaded chitosan nanoparticles |
| topic | aspirin nanoparticle drug release membrane guided bone regeneration |
| url | https://www.dovepress.com/guided-bone-regeneration-with-asymmetric-collagen-chitosan-membranes-c-peer-reviewed-article-IJN |
| work_keys_str_mv | AT zhangj guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT mas guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT liuz guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT gengh guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT lux guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT zhangx guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT lih guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT gaoc guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT zhangx guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles AT gaop guidedboneregenerationwithasymmetriccollagenchitosanmembranescontainingaspirinloadedchitosannanoparticles |