De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium Development
<i>Sclerotium rolfsii</i> is a destructive soil-borne fungal pathogen that causes stem rot in cultivated plants. However, little is known about the genetic basis of sclerotium development. In this study, we conducted de novo sequencing of genes from three different stages of <i>S....
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2023-12-01
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| author | Fanfan Wang Xiaoyue Wang Tao Tang Yuanyuan Duan Ting Mao Xiaoliang Guo Qingfang Wang Jingmao You |
| author_facet | Fanfan Wang Xiaoyue Wang Tao Tang Yuanyuan Duan Ting Mao Xiaoliang Guo Qingfang Wang Jingmao You |
| author_sort | Fanfan Wang |
| collection | DOAJ |
| description | <i>Sclerotium rolfsii</i> is a destructive soil-borne fungal pathogen that causes stem rot in cultivated plants. However, little is known about the genetic basis of sclerotium development. In this study, we conducted de novo sequencing of genes from three different stages of <i>S. rolfsii</i> (mycelia, early sclerotium formation, and late sclerotium formation) using Illumina HiSeq<sup>TM</sup> 4000. We then determined differentially expressed genes (DEGs) across the three stages and annotated gene functions. STEM and weighted gene-co-expression network analysis were used to cluster DEGs with similar expression patterns. Our analysis yielded an average of 25,957,621 clean reads per sample (22,913,500–28,988,848). We identified 8929, 8453, and 3744 DEGs between sclerotium developmental stages 1 versus 2, 1 versus 3, and 2 versus 3, respectively. Additionally, four significantly altered gene expression profiles involved 220 genes related to sclerotium formation, and two modules were positively correlated with early and late sclerotium formation. These results were supported by the outcomes of qPCR and RNA-sequencing conducted on six genes. This is the first study to provide a gene expression map during sclerotial development in <i>S. rolfsii</i>, which can be used to reduce the re-infection ability of this pathogen and provide new insights into the scientific prevention and control of the disease. This study also provides a useful resource for further research on the genomics of <i>S. rolfsii</i>. |
| first_indexed | 2024-03-08T20:44:28Z |
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| issn | 2073-4425 |
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| last_indexed | 2024-03-08T20:44:28Z |
| publishDate | 2023-12-01 |
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| series | Genes |
| spelling | doaj.art-1d5b67f6bdcd4cf888b3b2e536b3ec752023-12-22T14:11:10ZengMDPI AGGenes2073-44252023-12-011412217010.3390/genes14122170De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium DevelopmentFanfan Wang0Xiaoyue Wang1Tao Tang2Yuanyuan Duan3Ting Mao4Xiaoliang Guo5Qingfang Wang6Jingmao You7Key Laboratory of Biology and Cultivation of Chinese Herbal Medicines, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, ChinaKey Laboratory of Biology and Cultivation of Chinese Herbal Medicines, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, ChinaKey Laboratory of Biology and Cultivation of Chinese Herbal Medicines, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, ChinaHubei Engineering Research Center of Under-Forest Economy, Hubei Academy of Agricultural Sciences, Wuhan 430064, ChinaKey Laboratory of Biology and Cultivation of Chinese Herbal Medicines, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, ChinaKey Laboratory of Biology and Cultivation of Chinese Herbal Medicines, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, ChinaKey Laboratory of Biology and Cultivation of Chinese Herbal Medicines, Ministry of Agriculture and Rural Affairs, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, ChinaHubei Engineering Research Center of Good Agricultural Practices (GAP) Production for Chinese Herbal Medicines, Institute of Chinese Herbal Medicines, Hubei Academy of Agricultural Sciences, Enshi 445000, China<i>Sclerotium rolfsii</i> is a destructive soil-borne fungal pathogen that causes stem rot in cultivated plants. However, little is known about the genetic basis of sclerotium development. In this study, we conducted de novo sequencing of genes from three different stages of <i>S. rolfsii</i> (mycelia, early sclerotium formation, and late sclerotium formation) using Illumina HiSeq<sup>TM</sup> 4000. We then determined differentially expressed genes (DEGs) across the three stages and annotated gene functions. STEM and weighted gene-co-expression network analysis were used to cluster DEGs with similar expression patterns. Our analysis yielded an average of 25,957,621 clean reads per sample (22,913,500–28,988,848). We identified 8929, 8453, and 3744 DEGs between sclerotium developmental stages 1 versus 2, 1 versus 3, and 2 versus 3, respectively. Additionally, four significantly altered gene expression profiles involved 220 genes related to sclerotium formation, and two modules were positively correlated with early and late sclerotium formation. These results were supported by the outcomes of qPCR and RNA-sequencing conducted on six genes. This is the first study to provide a gene expression map during sclerotial development in <i>S. rolfsii</i>, which can be used to reduce the re-infection ability of this pathogen and provide new insights into the scientific prevention and control of the disease. This study also provides a useful resource for further research on the genomics of <i>S. rolfsii</i>.https://www.mdpi.com/2073-4425/14/12/2170<i>Sclerotium rolfsii</i>transcriptomesclerotiumweighted gene co-expression network analysis |
| spellingShingle | Fanfan Wang Xiaoyue Wang Tao Tang Yuanyuan Duan Ting Mao Xiaoliang Guo Qingfang Wang Jingmao You De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium Development Genes <i>Sclerotium rolfsii</i> transcriptome sclerotium weighted gene co-expression network analysis |
| title | De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium Development |
| title_full | De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium Development |
| title_fullStr | De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium Development |
| title_full_unstemmed | De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium Development |
| title_short | De Novo RNA Sequencing and Transcriptome Analysis of <i>Sclerotium rolfsii</i> Gene Expression during Sclerotium Development |
| title_sort | de novo rna sequencing and transcriptome analysis of i sclerotium rolfsii i gene expression during sclerotium development |
| topic | <i>Sclerotium rolfsii</i> transcriptome sclerotium weighted gene co-expression network analysis |
| url | https://www.mdpi.com/2073-4425/14/12/2170 |
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