PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation
Tumor metabolic reprogramming and epigenetic modification work together to promote tumorigenesis and development. Protein lysine acetylation, which affects a variety of biological functions of proteins, plays an important role under physiol...
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2023-08-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2023155 |
| _version_ | 1797635879400898560 |
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| author | Fu Gongyu Li Shi-Ting Jiang Zetan Mao Qiankun Xiong Nanchi Li Xiang Hao Yijie Zhang Huafeng |
| author_facet | Fu Gongyu Li Shi-Ting Jiang Zetan Mao Qiankun Xiong Nanchi Li Xiang Hao Yijie Zhang Huafeng |
| author_sort | Fu Gongyu |
| collection | DOAJ |
| description | Tumor metabolic reprogramming and epigenetic modification work together to promote tumorigenesis and development. Protein lysine acetylation, which affects a variety of biological functions of proteins, plays an important role under physiological and pathological conditions. Here, through immunoprecipitation and mass spectrum data, we show that phosphoglycerate mutase 5 (PGAM5) deacetylation enhances malic enzyme 1 (ME1) metabolic enzyme activity to promote lipid synthesis and proliferation of liver cancer cells. Mechanistically, we demonstrate that the deacetylase SIRT2 mediates PGAM5 deacetylation to activate ME1 activity, leading to ME1 dephosphorylation, subsequent lipid accumulation and the proliferation of liver cancer cells. Taken together, our study establishes an important role for the SIRT2-PGAM5-ME1 axis in the proliferation of liver cancer cells, suggesting a potential innovative cancer therapy. |
| first_indexed | 2024-03-11T12:26:24Z |
| format | Article |
| id | doaj.art-1d5f8f78ea80408ca35b6efb83d2f3e9 |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:26:24Z |
| publishDate | 2023-08-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-1d5f8f78ea80408ca35b6efb83d2f3e92023-11-06T08:30:38ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452023-08-01551370137910.3724/abbs.202315520d259ccPGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferationFu Gongyu0Li Shi-Ting1Jiang Zetan2Mao Qiankun3Xiong Nanchi4Li Xiang5Hao Yijie6Zhang Huafeng7["Anhui Key Laboratory of Hepatopancreatobiliary Surgery, Department of General Surgery, Anhui Provincial Hospital, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China","Hefei National Laboratory for Physical Sciences at Microscale, the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"]["Guangdong Cardiovascular Institute, Guangdong Provincial People’s Hospital, Guangdong Academy of Medical Sciences, Guangzhou 510080, China"]["Anhui Key Laboratory of Hepatopancreatobiliary Surgery, Department of General Surgery, Anhui Provincial Hospital, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China","Hefei National Laboratory for Physical Sciences at Microscale, the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"]["Anhui Key Laboratory of Hepatopancreatobiliary Surgery, Department of General Surgery, Anhui Provincial Hospital, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China","Hefei National Laboratory for Physical Sciences at Microscale, the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"]["Anhui Key Laboratory of Hepatopancreatobiliary Surgery, Department of General Surgery, Anhui Provincial Hospital, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China","Hefei National Laboratory for Physical Sciences at Microscale, the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"]["Hefei National Laboratory for Physical Sciences at Microscale, the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"]["Anhui Key Laboratory of Hepatopancreatobiliary Surgery, Department of General Surgery, Anhui Provincial Hospital, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China","Hefei National Laboratory for Physical Sciences at Microscale, the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"]["Anhui Key Laboratory of Hepatopancreatobiliary Surgery, Department of General Surgery, Anhui Provincial Hospital, the First Affiliated Hospital of USTC, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China","Hefei National Laboratory for Physical Sciences at Microscale, the Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic Disease, School of Basic Medical Sciences, Division of Life Science and Medicine, University of Science and Technology of China, Hefei 230027, China"]Tumor metabolic reprogramming and epigenetic modification work together to promote tumorigenesis and development. Protein lysine acetylation, which affects a variety of biological functions of proteins, plays an important role under physiological and pathological conditions. Here, through immunoprecipitation and mass spectrum data, we show that phosphoglycerate mutase 5 (PGAM5) deacetylation enhances malic enzyme 1 (ME1) metabolic enzyme activity to promote lipid synthesis and proliferation of liver cancer cells. Mechanistically, we demonstrate that the deacetylase SIRT2 mediates PGAM5 deacetylation to activate ME1 activity, leading to ME1 dephosphorylation, subsequent lipid accumulation and the proliferation of liver cancer cells. Taken together, our study establishes an important role for the SIRT2-PGAM5-ME1 axis in the proliferation of liver cancer cells, suggesting a potential innovative cancer therapy.https://www.sciengine.com/doi/10.3724/abbs.2023155metabolic reprogramingPGAM5protein acetylationSIRT2ME1liver cancer |
| spellingShingle | Fu Gongyu Li Shi-Ting Jiang Zetan Mao Qiankun Xiong Nanchi Li Xiang Hao Yijie Zhang Huafeng PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation Acta Biochimica et Biophysica Sinica metabolic reprograming PGAM5 protein acetylation SIRT2 ME1 liver cancer |
| title | PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation |
| title_full | PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation |
| title_fullStr | PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation |
| title_full_unstemmed | PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation |
| title_short | PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation |
| title_sort | pgam5 deacetylation mediated by sirt2 facilitates lipid metabolism and liver cancer proliferation |
| topic | metabolic reprograming PGAM5 protein acetylation SIRT2 ME1 liver cancer |
| url | https://www.sciengine.com/doi/10.3724/abbs.2023155 |
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