Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen
Recently discovered microcolonial forms of Mycoplasma hominis (M. hominis) and their impact on human spermatogenesis are studied. The spermatozoa of 125 fertile men (sperm donors; from Reprobank [Reproductive Tissue Bank, Moscow, Russia]) and of 93 patients with fertility problems (from the Federal...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-01-01
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| Series: | Asian Journal of Andrology |
| Subjects: | |
| Online Access: | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2024;volume=26;issue=1;spage=71;epage=76;aulast= |
| _version_ | 1797300067011395584 |
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| author | Elizaveta Bragina Tatyana Sorokina Avtandil Chogovadze Yulia Shevchenko Vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya |
| author_facet | Elizaveta Bragina Tatyana Sorokina Avtandil Chogovadze Yulia Shevchenko Vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya |
| author_sort | Elizaveta Bragina |
| collection | DOAJ |
| description | Recently discovered microcolonial forms of Mycoplasma hominis (M. hominis) and their impact on human spermatogenesis are studied. The spermatozoa of 125 fertile men (sperm donors; from Reprobank [Reproductive Tissue Bank, Moscow, Russia]) and of 93 patients with fertility problems (from the Federal State Budgetary Institution “Research Centre for Medical Genetics [RCMG]”, Moscow, Russia) were used. Classical colonies of M. hominis and microcolonies were detected by molecular biological methods, culture of bacteria, and transmission electron microscopy. The unique structure of microcolonial cells, in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12–14 nm, and the ability of microcolonial cells to attach to spermatozoa are shown. In patients with lower sperm quality, microcolonies of M. hominis were detected 2.5 times more frequently than classical colonies. The detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility. |
| first_indexed | 2024-03-07T23:00:51Z |
| format | Article |
| id | doaj.art-1d5fc685adbe4f20b99d421f4c9eb417 |
| institution | Directory Open Access Journal |
| issn | 1008-682X 1745-7262 |
| language | English |
| last_indexed | 2024-03-07T23:00:51Z |
| publishDate | 2024-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Andrology |
| spelling | doaj.art-1d5fc685adbe4f20b99d421f4c9eb4172024-02-22T14:40:20ZengWolters Kluwer Medknow PublicationsAsian Journal of Andrology1008-682X1745-72622024-01-01261717610.4103/aja202333Detection and evaluation of different morphological forms of Mycoplasma hominis in human semenElizaveta BraginaTatyana SorokinaAvtandil ChogovadzeYulia ShevchenkoVyacheslav ChernykhOlga BarkhatovaIrina RakovskayaRecently discovered microcolonial forms of Mycoplasma hominis (M. hominis) and their impact on human spermatogenesis are studied. The spermatozoa of 125 fertile men (sperm donors; from Reprobank [Reproductive Tissue Bank, Moscow, Russia]) and of 93 patients with fertility problems (from the Federal State Budgetary Institution “Research Centre for Medical Genetics [RCMG]”, Moscow, Russia) were used. Classical colonies of M. hominis and microcolonies were detected by molecular biological methods, culture of bacteria, and transmission electron microscopy. The unique structure of microcolonial cells, in which the cytoplasmic cylinder is surrounded by concentric electron-dense and electron-light layers with a periodicity of 12–14 nm, and the ability of microcolonial cells to attach to spermatozoa are shown. In patients with lower sperm quality, microcolonies of M. hominis were detected 2.5 times more frequently than classical colonies. The detection of microcolonies in the ejaculate and the frequent isolation of microcolonies from sperm samples of patients with fertility problems suggest that microcolonial cells may be one cause of infertility.http://www.ajandrology.com/article.asp?issn=1008-682X;year=2024;volume=26;issue=1;spage=71;epage=76;aulast=ejaculate; fertility; mycoplasma hominis; spermatogenesis; transmission electron microscopy |
| spellingShingle | Elizaveta Bragina Tatyana Sorokina Avtandil Chogovadze Yulia Shevchenko Vyacheslav Chernykh Olga Barkhatova Irina Rakovskaya Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen Asian Journal of Andrology ejaculate; fertility; mycoplasma hominis; spermatogenesis; transmission electron microscopy |
| title | Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen |
| title_full | Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen |
| title_fullStr | Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen |
| title_full_unstemmed | Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen |
| title_short | Detection and evaluation of different morphological forms of Mycoplasma hominis in human semen |
| title_sort | detection and evaluation of different morphological forms of mycoplasma hominis in human semen |
| topic | ejaculate; fertility; mycoplasma hominis; spermatogenesis; transmission electron microscopy |
| url | http://www.ajandrology.com/article.asp?issn=1008-682X;year=2024;volume=26;issue=1;spage=71;epage=76;aulast= |
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