Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer
Deciphering the mechanisms of axis formation in amphioxus is a key step to understanding the evolution of chordate body plan. The current view is that Nodal signaling is the only factor promoting the dorsal axis specification in the amphioxus, whereas Wnt/β-catenin signaling plays no role in this pr...
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2020-05-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/56817 |
| _version_ | 1811181016163811328 |
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| author | Iryna Kozmikova Zbynek Kozmik |
| author_facet | Iryna Kozmikova Zbynek Kozmik |
| author_sort | Iryna Kozmikova |
| collection | DOAJ |
| description | Deciphering the mechanisms of axis formation in amphioxus is a key step to understanding the evolution of chordate body plan. The current view is that Nodal signaling is the only factor promoting the dorsal axis specification in the amphioxus, whereas Wnt/β-catenin signaling plays no role in this process. Here, we re-examined the role of Wnt/βcatenin signaling in the dorsal/ventral patterning of amphioxus embryo. We demonstrated that the spatial activity of Wnt/β-catenin signaling is located in presumptive dorsal cells from cleavage to gastrula stage, and provided functional evidence that Wnt/β-catenin signaling is necessary for the specification of dorsal cell fate in a stage-dependent manner. Microinjection of Wnt8 and Wnt11 mRNA induced ectopic dorsal axis in neurulae and larvae. Finally, we demonstrated that Nodal and Wnt/β-catenin signaling cooperate to promote the dorsal-specific gene expression in amphioxus gastrula. Our study reveals high evolutionary conservation of dorsal organizer formation in the chordate lineage. |
| first_indexed | 2024-04-11T09:11:15Z |
| format | Article |
| id | doaj.art-1d627f341a31425ebeb57b37c8c504ac |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:11:15Z |
| publishDate | 2020-05-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-1d627f341a31425ebeb57b37c8c504ac2022-12-22T04:32:29ZengeLife Sciences Publications LtdeLife2050-084X2020-05-01910.7554/eLife.56817Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizerIryna Kozmikova0https://orcid.org/0000-0002-7861-9802Zbynek Kozmik1Laboratory of Transcriptional Regulation, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech RepublicLaboratory of Transcriptional Regulation, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech RepublicDeciphering the mechanisms of axis formation in amphioxus is a key step to understanding the evolution of chordate body plan. The current view is that Nodal signaling is the only factor promoting the dorsal axis specification in the amphioxus, whereas Wnt/β-catenin signaling plays no role in this process. Here, we re-examined the role of Wnt/βcatenin signaling in the dorsal/ventral patterning of amphioxus embryo. We demonstrated that the spatial activity of Wnt/β-catenin signaling is located in presumptive dorsal cells from cleavage to gastrula stage, and provided functional evidence that Wnt/β-catenin signaling is necessary for the specification of dorsal cell fate in a stage-dependent manner. Microinjection of Wnt8 and Wnt11 mRNA induced ectopic dorsal axis in neurulae and larvae. Finally, we demonstrated that Nodal and Wnt/β-catenin signaling cooperate to promote the dorsal-specific gene expression in amphioxus gastrula. Our study reveals high evolutionary conservation of dorsal organizer formation in the chordate lineage.https://elifesciences.org/articles/56817body planaxial patterningevolutionwnt/β-catenin signalingbranchiostoma floridaebranchiostoma lanceolatum |
| spellingShingle | Iryna Kozmikova Zbynek Kozmik Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer eLife body plan axial patterning evolution wnt/β-catenin signaling branchiostoma floridae branchiostoma lanceolatum |
| title | Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer |
| title_full | Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer |
| title_fullStr | Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer |
| title_full_unstemmed | Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer |
| title_short | Wnt/β-catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer |
| title_sort | wnt β catenin signaling is an evolutionarily conserved determinant of chordate dorsal organizer |
| topic | body plan axial patterning evolution wnt/β-catenin signaling branchiostoma floridae branchiostoma lanceolatum |
| url | https://elifesciences.org/articles/56817 |
| work_keys_str_mv | AT irynakozmikova wntbcateninsignalingisanevolutionarilyconserveddeterminantofchordatedorsalorganizer AT zbynekkozmik wntbcateninsignalingisanevolutionarilyconserveddeterminantofchordatedorsalorganizer |