The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic Bacteria
The antibacterial agent can be extracted from plants, animals, and microorganisms such as fungi. The potential antibacterial activity of laccase derived from fungi remains limited in current reports. This study aimed to investigate the characteristics of laccase from Indonesian white rot fungi (WRF...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2023-10-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/47888 |
| _version_ | 1797642622960926720 |
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| author | Sita Heris Anita Deni Zulfiana Ananda Digita Nafisah Nuha Vilya Syafriana Amelia Febriani Dede Heri Yuli Yanto |
| author_facet | Sita Heris Anita Deni Zulfiana Ananda Digita Nafisah Nuha Vilya Syafriana Amelia Febriani Dede Heri Yuli Yanto |
| author_sort | Sita Heris Anita |
| collection | DOAJ |
| description |
The antibacterial agent can be extracted from plants, animals, and microorganisms such as fungi. The potential antibacterial activity of laccase derived from fungi remains limited in current reports. This study aimed to investigate the characteristics of laccase from Indonesian white rot fungi (WRF) and explore its potential as an antibacterial agent. The laccases were produced by Trametes hirsuta D7, Trametes hirsuta EDN 082, Leiotrametes menziesii BRB 73, and Lentinus sajor-caju BRB 12 using oil palm empty fruit bunch as a substrate. The results showed that the Indonesian WRF tested produced brownish-yellow laccase. FTIR analysis demonstrated similar peak patterns but distinct absorption intensities among the laccases. Trametes hirsuta D7 gained 0.044 U/ml of the greatest laccase activity. Laccase, with minimal activity of 0.001 U/ml–0.026 U/ml, suppressed the propagation of Propionibacterium acnes and Staphylococcus aureus. Escherichia coli and Pseudomonas aeruginosa could be inhibited by the laccase with a minimum activity of 0.002 U/ml–0.044 U/ml. However, S. aureus and E. coli showed the Minimum Bactericidal Concentration in the laccase activity range of 0.018 U/ml–0.308 U/ml. Gram-positive and Gram-negative bacteria grow more slowly when the laccase is present, supposed the laccase as a potential antibacterial agent.
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| first_indexed | 2024-03-11T14:02:59Z |
| format | Article |
| id | doaj.art-1d673ab123784855b149ae4f28b9801b |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-03-11T14:02:59Z |
| publishDate | 2023-10-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-1d673ab123784855b149ae4f28b9801b2023-11-02T03:37:56ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942023-10-0131110.4308/hjb.31.1.133-144The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic BacteriaSita Heris Anita0Deni Zulfiana1Ananda Digita2Nafisah Nuha3Vilya Syafriana4Amelia Febriani5Dede Heri Yuli Yanto6Research Center for Applied Microbiology, National Research and Innovation Agency Republic of Indonesia, Cibinong, IndonesiaResearch Center for Applied Microbiology, National Research and Innovation Agency Republic of Indonesia, Cibinong, IndonesiaDepartment of Pharmacy, National Institute of Science and Technology, South Jakarta, Jakarta Capital Special Region, IndonesiaDepartment of Pharmacy, National Institute of Science and Technology, South Jakarta, Jakarta Capital Special Region, IndonesiaDepartment of Pharmacy, National Institute of Science and Technology, South Jakarta, Jakarta Capital Special Region, IndonesiaDepartment of Pharmacy, National Institute of Science and Technology, South Jakarta, Jakarta Capital Special Region, IndonesiaResearch Center for Applied Microbiology, National Research and Innovation Agency Republic of Indonesia, Cibinong, Indonesia The antibacterial agent can be extracted from plants, animals, and microorganisms such as fungi. The potential antibacterial activity of laccase derived from fungi remains limited in current reports. This study aimed to investigate the characteristics of laccase from Indonesian white rot fungi (WRF) and explore its potential as an antibacterial agent. The laccases were produced by Trametes hirsuta D7, Trametes hirsuta EDN 082, Leiotrametes menziesii BRB 73, and Lentinus sajor-caju BRB 12 using oil palm empty fruit bunch as a substrate. The results showed that the Indonesian WRF tested produced brownish-yellow laccase. FTIR analysis demonstrated similar peak patterns but distinct absorption intensities among the laccases. Trametes hirsuta D7 gained 0.044 U/ml of the greatest laccase activity. Laccase, with minimal activity of 0.001 U/ml–0.026 U/ml, suppressed the propagation of Propionibacterium acnes and Staphylococcus aureus. Escherichia coli and Pseudomonas aeruginosa could be inhibited by the laccase with a minimum activity of 0.002 U/ml–0.044 U/ml. However, S. aureus and E. coli showed the Minimum Bactericidal Concentration in the laccase activity range of 0.018 U/ml–0.308 U/ml. Gram-positive and Gram-negative bacteria grow more slowly when the laccase is present, supposed the laccase as a potential antibacterial agent. https://journal.ipb.ac.id/index.php/hayati/article/view/47888 |
| spellingShingle | Sita Heris Anita Deni Zulfiana Ananda Digita Nafisah Nuha Vilya Syafriana Amelia Febriani Dede Heri Yuli Yanto The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic Bacteria Hayati Journal of Biosciences |
| title | The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic Bacteria |
| title_full | The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic Bacteria |
| title_fullStr | The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic Bacteria |
| title_full_unstemmed | The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic Bacteria |
| title_short | The First Evidence of Potential Antibacterial Activity of Laccase Enzyme from Indonesian White Rot Fungi against Pathogenic Bacteria |
| title_sort | first evidence of potential antibacterial activity of laccase enzyme from indonesian white rot fungi against pathogenic bacteria |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/47888 |
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