Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity
This study aimed to select the most effective culture extracts for controlling honeybee nosemosis using 342 entomopathogenic fungi of 24 species from 18 genera. The germination inhibitory activity of the fungal culture extract on <i>Nosema ceranae</i> spores was evaluated using an in vit...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-06-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/6/538 |
| _version_ | 1797594188476317696 |
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| author | Dong-Jun Kim Ra-Mi Woo Kyu-Seek Kim Soo-Dong Woo |
| author_facet | Dong-Jun Kim Ra-Mi Woo Kyu-Seek Kim Soo-Dong Woo |
| author_sort | Dong-Jun Kim |
| collection | DOAJ |
| description | This study aimed to select the most effective culture extracts for controlling honeybee nosemosis using 342 entomopathogenic fungi of 24 species from 18 genera. The germination inhibitory activity of the fungal culture extract on <i>Nosema ceranae</i> spores was evaluated using an in vitro germination assay method. Among 89 fungal culture extracts showing germination inhibitory activity of approximately 80% or more, 44 fungal culture extracts that maintained their inhibitory activity even at a concentration of 1% were selected. Finally, the honeybee nosemosis inhibitory activity was evaluated using the cultured extracts of five fungal isolates having a <i>Nosema</i> inhibitory activity of approximately 60% or more, even when the extract was removed after treatment. As a result, the proliferation of <i>Nosema</i> spores was reduced by all fungal culture extract treatments. However, only the treatment of the culture extracts from <i>Paecilomyces marquandii</i> 364 and <i>Pochonia bulbillosa</i> 60 showed a reduction in honeybee mortality due to nosemosis. In particular, the extracts of these two fungal isolates also increased the survival of honeybees. |
| first_indexed | 2024-03-11T02:19:06Z |
| format | Article |
| id | doaj.art-1d73073f72954f62a32fe690e4b6f965 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T02:19:06Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-1d73073f72954f62a32fe690e4b6f9652023-11-18T10:56:12ZengMDPI AGInsects2075-44502023-06-0114653810.3390/insects14060538Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory ActivityDong-Jun Kim0Ra-Mi Woo1Kyu-Seek Kim2Soo-Dong Woo3Department of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University, Cheongju 28644, Republic of KoreaDepartment of Agricultural Biology, College of Agriculture, Life & Environment Science, Chungbuk National University, Cheongju 28644, Republic of KoreaThis study aimed to select the most effective culture extracts for controlling honeybee nosemosis using 342 entomopathogenic fungi of 24 species from 18 genera. The germination inhibitory activity of the fungal culture extract on <i>Nosema ceranae</i> spores was evaluated using an in vitro germination assay method. Among 89 fungal culture extracts showing germination inhibitory activity of approximately 80% or more, 44 fungal culture extracts that maintained their inhibitory activity even at a concentration of 1% were selected. Finally, the honeybee nosemosis inhibitory activity was evaluated using the cultured extracts of five fungal isolates having a <i>Nosema</i> inhibitory activity of approximately 60% or more, even when the extract was removed after treatment. As a result, the proliferation of <i>Nosema</i> spores was reduced by all fungal culture extract treatments. However, only the treatment of the culture extracts from <i>Paecilomyces marquandii</i> 364 and <i>Pochonia bulbillosa</i> 60 showed a reduction in honeybee mortality due to nosemosis. In particular, the extracts of these two fungal isolates also increased the survival of honeybees.https://www.mdpi.com/2075-4450/14/6/538honeybeenosemosis<i>Nosema ceranae</i><i>Apis mellifera</i>entomopathogenic fungiculture extracts |
| spellingShingle | Dong-Jun Kim Ra-Mi Woo Kyu-Seek Kim Soo-Dong Woo Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity Insects honeybee nosemosis <i>Nosema ceranae</i> <i>Apis mellifera</i> entomopathogenic fungi culture extracts |
| title | Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity |
| title_full | Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity |
| title_fullStr | Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity |
| title_full_unstemmed | Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity |
| title_short | Screening of Entomopathogenic Fungal Culture Extracts with Honeybee Nosemosis Inhibitory Activity |
| title_sort | screening of entomopathogenic fungal culture extracts with honeybee nosemosis inhibitory activity |
| topic | honeybee nosemosis <i>Nosema ceranae</i> <i>Apis mellifera</i> entomopathogenic fungi culture extracts |
| url | https://www.mdpi.com/2075-4450/14/6/538 |
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