PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry
Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting a valuable p...
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MDPI AG
2019-02-01
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| Online Access: | https://www.mdpi.com/1996-1944/12/4/597 |
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| author | Marco Tatullo Gianrico Spagnuolo Bruna Codispoti Fausto Zamparini Anqi Zhang Micaela Degli Esposti Conrado Aparicio Carlo Rengo Manuel Nuzzolese Lucia Manzoli Fabio Fava Carlo Prati Paola Fabbri Maria Giovanna Gandolfi |
| author_facet | Marco Tatullo Gianrico Spagnuolo Bruna Codispoti Fausto Zamparini Anqi Zhang Micaela Degli Esposti Conrado Aparicio Carlo Rengo Manuel Nuzzolese Lucia Manzoli Fabio Fava Carlo Prati Paola Fabbri Maria Giovanna Gandolfi |
| author_sort | Marco Tatullo |
| collection | DOAJ |
| description | Human periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting a valuable potential in tissue engineering and regenerative medicine applications. In the present study, hPCy-MSCs were collected, isolated, and seeded on three experimental mineral-doped porous scaffolds produced by the thermally-induced phase-separation (TIPS) technique. Mineral-doped scaffolds, composed of polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD), and/or hydraulic calcium silicate (CaSi), were produced by TIPS (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Micro-CT analysis evaluated scaffolds micromorphology. Collected hPCy-MSCs, characterized by cytofluorimetry, were seeded on the scaffolds and tested for cell proliferation, cells viability, and gene expression for osteogenic and odontogenic differentiation (DMP-1, OSC, RUNX-2, HPRT). Micro-CT revealed an interconnected highly porous structure for all the scaffolds, similar total porosity with 99% open pores. Pore wall thickness increased with the percentage of CaSi and DCPD. Cells seeded on mineral-doped scaffolds showed a superior proliferation compared to pure PLA scaffolds (control), particularly on PLA-10CaSi-10DCPD at day 12. A higher number of non-viable (red stained) cells was observable on PLA scaffolds at days 14 and 21. DMP-1 expression increased in hPCy-MSCs cultured on all mineral-doped scaffolds, in particular on PLA-5CaSi-5DCPD and PLA-10CaSi-10DCPD. In conclusion, the innovative combination of experimental scaffolds colonized with autologous stem cells from periapical cyst represent a promising strategy for regenerative healing of periapical and alveolar bone. |
| first_indexed | 2024-04-13T06:15:57Z |
| format | Article |
| id | doaj.art-1d76e4b8866843b5a457b83c24a9de5f |
| institution | Directory Open Access Journal |
| issn | 1996-1944 |
| language | English |
| last_indexed | 2024-04-13T06:15:57Z |
| publishDate | 2019-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Materials |
| spelling | doaj.art-1d76e4b8866843b5a457b83c24a9de5f2022-12-22T02:58:49ZengMDPI AGMaterials1996-19442019-02-0112459710.3390/ma12040597ma12040597PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in DentistryMarco Tatullo0Gianrico Spagnuolo1Bruna Codispoti2Fausto Zamparini3Anqi Zhang4Micaela Degli Esposti5Conrado Aparicio6Carlo Rengo7Manuel Nuzzolese8Lucia Manzoli9Fabio Fava10Carlo Prati11Paola Fabbri12Maria Giovanna Gandolfi13Tecnologica Research Institute, Stem Cell Unit, 88900 Crotone, ItalyDepartment of Neurosciences, Reproductive and Odontostomatological Sciences, University of Naples “Federico II”, 80131 Napoli, ItalyTecnologica Research Institute, Stem Cell Unit, 88900 Crotone, ItalyLaboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, ItalyMDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USADepartment of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, 40131 Bologna, ItalyMDRCBB-Minnesota Dental Research Center for Biomaterials and Biomechanics, School of Dentistry, University of Minnesota, Minneapolis, MN 55455, USADepartment of Prosthodontics and Dental Materials, School of Dental Medicine, University of Siena, 53100 Siena, ItalyUniversity Hospitals Birmingham—NHS Foundation Trust, Birmingham B152GW, UKCellular Signalling Laboratory, Institute of Human Anatomy, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40126 Bologna, ItalyDepartment of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, 40131 Bologna, ItalyLaboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, ItalyDepartment of Civil, Chemical, Environmental and Materials Engineering, University of Bologna, 40131 Bologna, ItalyLaboratory of Biomaterials and Oral Pathology, School of Dentistry, Department of Biomedical and Neuromotor Sciences, University of Bologna, 40125 Bologna, ItalyHuman periapical cyst mesenchymal stem cells (hPCy-MSCs) are a newly discovered cell population innovatively collected from inflammatory periapical cysts. The use of this biological waste guarantees a source of stem cells without any impact on the surrounding healthy tissues, presenting a valuable potential in tissue engineering and regenerative medicine applications. In the present study, hPCy-MSCs were collected, isolated, and seeded on three experimental mineral-doped porous scaffolds produced by the thermally-induced phase-separation (TIPS) technique. Mineral-doped scaffolds, composed of polylactic acid (PLA), dicalcium phosphate dihydrate (DCPD), and/or hydraulic calcium silicate (CaSi), were produced by TIPS (PLA-10CaSi, PLA-5CaSi-5DCPD, PLA-10CaSi-10DCPD). Micro-CT analysis evaluated scaffolds micromorphology. Collected hPCy-MSCs, characterized by cytofluorimetry, were seeded on the scaffolds and tested for cell proliferation, cells viability, and gene expression for osteogenic and odontogenic differentiation (DMP-1, OSC, RUNX-2, HPRT). Micro-CT revealed an interconnected highly porous structure for all the scaffolds, similar total porosity with 99% open pores. Pore wall thickness increased with the percentage of CaSi and DCPD. Cells seeded on mineral-doped scaffolds showed a superior proliferation compared to pure PLA scaffolds (control), particularly on PLA-10CaSi-10DCPD at day 12. A higher number of non-viable (red stained) cells was observable on PLA scaffolds at days 14 and 21. DMP-1 expression increased in hPCy-MSCs cultured on all mineral-doped scaffolds, in particular on PLA-5CaSi-5DCPD and PLA-10CaSi-10DCPD. In conclusion, the innovative combination of experimental scaffolds colonized with autologous stem cells from periapical cyst represent a promising strategy for regenerative healing of periapical and alveolar bone.https://www.mdpi.com/1996-1944/12/4/597polylactic acid (PLA)-based scaffoldscalcium silicate-containing scaffoldsbioactive scaffoldsporous scaffoldshuman periapical cyst mesenchymal stem cells (hPCy-MSCs)regenerative dentistrythermally induced phase separation technique (TIPS) |
| spellingShingle | Marco Tatullo Gianrico Spagnuolo Bruna Codispoti Fausto Zamparini Anqi Zhang Micaela Degli Esposti Conrado Aparicio Carlo Rengo Manuel Nuzzolese Lucia Manzoli Fabio Fava Carlo Prati Paola Fabbri Maria Giovanna Gandolfi PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry Materials polylactic acid (PLA)-based scaffolds calcium silicate-containing scaffolds bioactive scaffolds porous scaffolds human periapical cyst mesenchymal stem cells (hPCy-MSCs) regenerative dentistry thermally induced phase separation technique (TIPS) |
| title | PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry |
| title_full | PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry |
| title_fullStr | PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry |
| title_full_unstemmed | PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry |
| title_short | PLA-Based Mineral-Doped Scaffolds Seeded with Human Periapical Cyst-Derived MSCs: A Promising Tool for Regenerative Healing in Dentistry |
| title_sort | pla based mineral doped scaffolds seeded with human periapical cyst derived mscs a promising tool for regenerative healing in dentistry |
| topic | polylactic acid (PLA)-based scaffolds calcium silicate-containing scaffolds bioactive scaffolds porous scaffolds human periapical cyst mesenchymal stem cells (hPCy-MSCs) regenerative dentistry thermally induced phase separation technique (TIPS) |
| url | https://www.mdpi.com/1996-1944/12/4/597 |
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