Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan
Triclosan, produced as a broad-spectrum antibiotic in the early 1960s, is generally used as a preservative in personal care products, fabrics, plastic products such as kitchenware and toys. As a result of the high demand for triclosan, this chemical threatens the aquatic ecosystem by contaminating w...
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| Format: | Article |
| Language: | English |
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University of Belgrade, University of Novi Sad
2021-01-01
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| Series: | Archives of Biological Sciences |
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| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642100040A.pdf |
| _version_ | 1818950376391442432 |
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| author | Akbulut Cansu |
| author_facet | Akbulut Cansu |
| author_sort | Akbulut Cansu |
| collection | DOAJ |
| description | Triclosan, produced as a broad-spectrum antibiotic in the early 1960s, is generally used as a preservative in personal care products, fabrics, plastic products such as kitchenware and toys. As a result of the high demand for triclosan, this chemical threatens the aquatic ecosystem by contaminating wastewater sources. Environmental pollutants affect the reproductive potential of fish, one of the most critical aquatic organisms. This study aimed to investigate the histopathological and apoptotic effects of triclosan in zebrafish gonads. Fish were exposed to sublethal concentrations of triclosan for 5 days, and general histological methods were applied. Histological sections were examined under a light microscope after staining with hematoxylin and eosin and toluidine blue. Triclosan exposure caused deterioration in ovarian tissue, such as shrinkage in the ooplasm, accumulation of proteinaceous fluid in the interstitial tissue, morphological changes of oocyte and the zona radiata. In testicular tissue, triclosan exposure caused fusion in seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, edema in seminiferous tubules, and karyorrhexis in spermatogenic cells. The TUNEL assay was used for the determination of apoptotic cells. Brown-colored apoptotic cells were visualized under the light microscope. TUNEL positive cells were observed in all exposure groups. Triclosan administration was found to cause apoptosis in zebrafish gonads. These findings indicate that triclosan potentially affects fish reproduction, and that its judicious disposal is essential for protecting the environment and maintaining the reproductive potential of fish. |
| first_indexed | 2024-12-20T09:17:36Z |
| format | Article |
| id | doaj.art-1d78c255a89d47e58cf4fcbdfb028e0b |
| institution | Directory Open Access Journal |
| issn | 0354-4664 1821-4339 |
| language | English |
| last_indexed | 2024-12-20T09:17:36Z |
| publishDate | 2021-01-01 |
| publisher | University of Belgrade, University of Novi Sad |
| record_format | Article |
| series | Archives of Biological Sciences |
| spelling | doaj.art-1d78c255a89d47e58cf4fcbdfb028e0b2022-12-21T19:45:22ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392021-01-0173446547210.2298/ABS210923040A0354-46642100040AHistopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosanAkbulut Cansu0Sakarya University Science and Letters Faculty Department of Biology, Serdivan Sakarya, TurkeyTriclosan, produced as a broad-spectrum antibiotic in the early 1960s, is generally used as a preservative in personal care products, fabrics, plastic products such as kitchenware and toys. As a result of the high demand for triclosan, this chemical threatens the aquatic ecosystem by contaminating wastewater sources. Environmental pollutants affect the reproductive potential of fish, one of the most critical aquatic organisms. This study aimed to investigate the histopathological and apoptotic effects of triclosan in zebrafish gonads. Fish were exposed to sublethal concentrations of triclosan for 5 days, and general histological methods were applied. Histological sections were examined under a light microscope after staining with hematoxylin and eosin and toluidine blue. Triclosan exposure caused deterioration in ovarian tissue, such as shrinkage in the ooplasm, accumulation of proteinaceous fluid in the interstitial tissue, morphological changes of oocyte and the zona radiata. In testicular tissue, triclosan exposure caused fusion in seminiferous tubules, hypertrophy in spermatogenic and Leydig cells, edema in seminiferous tubules, and karyorrhexis in spermatogenic cells. The TUNEL assay was used for the determination of apoptotic cells. Brown-colored apoptotic cells were visualized under the light microscope. TUNEL positive cells were observed in all exposure groups. Triclosan administration was found to cause apoptosis in zebrafish gonads. These findings indicate that triclosan potentially affects fish reproduction, and that its judicious disposal is essential for protecting the environment and maintaining the reproductive potential of fish.http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642100040A.pdftriclosantestisovarytunel assayzebrafish |
| spellingShingle | Akbulut Cansu Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan Archives of Biological Sciences triclosan testis ovary tunel assay zebrafish |
| title | Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan |
| title_full | Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan |
| title_fullStr | Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan |
| title_full_unstemmed | Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan |
| title_short | Histopathological and apoptotic examination of zebrafish (Danio rerio) gonads exposed to triclosan |
| title_sort | histopathological and apoptotic examination of zebrafish danio rerio gonads exposed to triclosan |
| topic | triclosan testis ovary tunel assay zebrafish |
| url | http://www.doiserbia.nb.rs/img/doi/0354-4664/2021/0354-46642100040A.pdf |
| work_keys_str_mv | AT akbulutcansu histopathologicalandapoptoticexaminationofzebrafishdanioreriogonadsexposedtotriclosan |