Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cells
Mastl is an essential kinase required for inhibition of the phosphatase activity directed toward Cdk1/cyclin B substrates during mitosis. Mastl phosphorylates two small evolutionarily conserved proteins, Arpp19 and Ensa converting them into strong inhibitors of PP2A-B55δ. Mastl-Arpp19/Ensa-PP2A regu...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-03-01
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| Series: | Türk Biyokimya Dergisi |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/tjb-2022-0191 |
| _version_ | 1797814124854378496 |
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| author | Diril Muhammed Kasim Erguven Mehmet |
| author_facet | Diril Muhammed Kasim Erguven Mehmet |
| author_sort | Diril Muhammed Kasim |
| collection | DOAJ |
| description | Mastl is an essential kinase required for inhibition of the phosphatase activity directed toward Cdk1/cyclin B substrates during mitosis. Mastl phosphorylates two small evolutionarily conserved proteins, Arpp19 and Ensa converting them into strong inhibitors of PP2A-B55δ. Mastl-Arpp19/Ensa-PP2A regulatory pathway has been mainly studied in Xenopus egg extracts and Drosophila using biochemical and genetic approaches. Studies in mammalian cells and genetically modified mouse models have suggested distinct but important functions for Arpp19 and Ensa, in mitosis and S-phase, respectively. A detailed comparative analysis of the Arpp19 and Ensa functions in mammalian cells has not been performed. |
| first_indexed | 2024-03-13T08:02:55Z |
| format | Article |
| id | doaj.art-1d7adfc1d5c2499c972814fc1513c004 |
| institution | Directory Open Access Journal |
| issn | 1303-829X |
| language | English |
| last_indexed | 2024-03-13T08:02:55Z |
| publishDate | 2023-03-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Türk Biyokimya Dergisi |
| spelling | doaj.art-1d7adfc1d5c2499c972814fc1513c0042023-06-01T09:43:54ZengDe GruyterTürk Biyokimya Dergisi1303-829X2023-03-0148219020210.1515/tjb-2022-0191Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cellsDiril Muhammed Kasim0Erguven Mehmet1Izmir Biomedicine and Genome Center, Balcova, Izmir, TürkiyeIzmir Biomedicine and Genome Center, Balcova, Izmir, TürkiyeMastl is an essential kinase required for inhibition of the phosphatase activity directed toward Cdk1/cyclin B substrates during mitosis. Mastl phosphorylates two small evolutionarily conserved proteins, Arpp19 and Ensa converting them into strong inhibitors of PP2A-B55δ. Mastl-Arpp19/Ensa-PP2A regulatory pathway has been mainly studied in Xenopus egg extracts and Drosophila using biochemical and genetic approaches. Studies in mammalian cells and genetically modified mouse models have suggested distinct but important functions for Arpp19 and Ensa, in mitosis and S-phase, respectively. A detailed comparative analysis of the Arpp19 and Ensa functions in mammalian cells has not been performed.https://doi.org/10.1515/tjb-2022-0191arpp19ensagreatwallmastlmitosispp2a-b55δ |
| spellingShingle | Diril Muhammed Kasim Erguven Mehmet Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cells Türk Biyokimya Dergisi arpp19 ensa greatwall mastl mitosis pp2a-b55δ |
| title | Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cells |
| title_full | Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cells |
| title_fullStr | Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cells |
| title_full_unstemmed | Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cells |
| title_short | Genetic dissection of the Mastl-Arpp19/Ensa-PP2A-B55δ pathway in mammalian cells |
| title_sort | genetic dissection of the mastl arpp19 ensa pp2a b55δ pathway in mammalian cells |
| topic | arpp19 ensa greatwall mastl mitosis pp2a-b55δ |
| url | https://doi.org/10.1515/tjb-2022-0191 |
| work_keys_str_mv | AT dirilmuhammedkasim geneticdissectionofthemastlarpp19ensapp2ab55dpathwayinmammaliancells AT erguvenmehmet geneticdissectionofthemastlarpp19ensapp2ab55dpathwayinmammaliancells |