Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i>
The vigor of tea plants (<i>Camellia sinensis</i>) and tea quality are strongly influenced by the abundance and forms of nitrogen, principally NO<sub>3</sub><sup>−</sup>, NH<sub>4</sub><sup>+</sup>, and amino acids. Mechanisms to access dif...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Plants |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2223-7747/9/9/1218 |
| _version_ | 1827706062665416704 |
|---|---|
| author | Xinwan Zhang Hongling Liu Elizabeth Pilon-Smits Wei Huang Pu Wang Mingle Wang Fei Guo Yu Wang Ruiyuan Li Hua Zhao Dejiang Ni |
| author_facet | Xinwan Zhang Hongling Liu Elizabeth Pilon-Smits Wei Huang Pu Wang Mingle Wang Fei Guo Yu Wang Ruiyuan Li Hua Zhao Dejiang Ni |
| author_sort | Xinwan Zhang |
| collection | DOAJ |
| description | The vigor of tea plants (<i>Camellia sinensis</i>) and tea quality are strongly influenced by the abundance and forms of nitrogen, principally NO<sub>3</sub><sup>−</sup>, NH<sub>4</sub><sup>+</sup>, and amino acids. Mechanisms to access different nitrogen sources and the regulatory cues remain largely elusive in tea plants. A transcriptome analysis was performed to categorize differentially expressed genes (DEGs) in roots and young leaves during the early response to four nitrogen treatments. Relative to the continuously nitrogen-replete control, the three nitrogen-deprived and resupplied treatments shared 237 DEGs in the shoots and 21 DEGs in the root. Gene-ontology characterization revealed that transcripts encoding genes predicted to participate in nitrogen uptake, assimilation, and translocation were among the most differentially expressed after exposure to the different nitrogen regimes. Because of its high transcript level regardless of nitrogen condition, a putative amino acid transporter, TEA020444/<i>CsCAT9.1</i>, was further characterized in <i>Arabidopsis</i> and found to mediate the acquisition of a broad spectrum of amino acids, suggesting a role in amino acid uptake, transport, and deposition in sinks as an internal reservoir. Our results enhance our understanding of nitrogen-regulated transcript level patterns in tea plants and pinpoint candidate genes that function in nitrogen transport and metabolism, allowing tea plants to adjust to variable nitrogen environments. |
| first_indexed | 2024-03-10T16:16:14Z |
| format | Article |
| id | doaj.art-1d7f9d85077b478381d36b60ee52e13f |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T16:16:14Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1d7f9d85077b478381d36b60ee52e13f2023-11-20T14:01:41ZengMDPI AGPlants2223-77472020-09-0199121810.3390/plants9091218Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i>Xinwan Zhang0Hongling Liu1Elizabeth Pilon-Smits2Wei Huang3Pu Wang4Mingle Wang5Fei Guo6Yu Wang7Ruiyuan Li8Hua Zhao9Dejiang Ni10Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaDepartment of Biology, Colorado State University, Fort Collins, CO 80521, USAKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of information and computing science Guizhou Province, Guizhou Normal University, Guiyang 550001, ChinaKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaKey Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, Wuhan 430070, ChinaThe vigor of tea plants (<i>Camellia sinensis</i>) and tea quality are strongly influenced by the abundance and forms of nitrogen, principally NO<sub>3</sub><sup>−</sup>, NH<sub>4</sub><sup>+</sup>, and amino acids. Mechanisms to access different nitrogen sources and the regulatory cues remain largely elusive in tea plants. A transcriptome analysis was performed to categorize differentially expressed genes (DEGs) in roots and young leaves during the early response to four nitrogen treatments. Relative to the continuously nitrogen-replete control, the three nitrogen-deprived and resupplied treatments shared 237 DEGs in the shoots and 21 DEGs in the root. Gene-ontology characterization revealed that transcripts encoding genes predicted to participate in nitrogen uptake, assimilation, and translocation were among the most differentially expressed after exposure to the different nitrogen regimes. Because of its high transcript level regardless of nitrogen condition, a putative amino acid transporter, TEA020444/<i>CsCAT9.1</i>, was further characterized in <i>Arabidopsis</i> and found to mediate the acquisition of a broad spectrum of amino acids, suggesting a role in amino acid uptake, transport, and deposition in sinks as an internal reservoir. Our results enhance our understanding of nitrogen-regulated transcript level patterns in tea plants and pinpoint candidate genes that function in nitrogen transport and metabolism, allowing tea plants to adjust to variable nitrogen environments.https://www.mdpi.com/2223-7747/9/9/1218assimilation<i>Camellia sinensis</i>nitrogentransporttranscriptome |
| spellingShingle | Xinwan Zhang Hongling Liu Elizabeth Pilon-Smits Wei Huang Pu Wang Mingle Wang Fei Guo Yu Wang Ruiyuan Li Hua Zhao Dejiang Ni Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i> Plants assimilation <i>Camellia sinensis</i> nitrogen transport transcriptome |
| title | Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i> |
| title_full | Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i> |
| title_fullStr | Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i> |
| title_full_unstemmed | Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i> |
| title_short | Transcriptome-Wide Analysis of Nitrogen-Regulated Genes in Tea Plant (<i>Camellia sinensis</i> L. O. Kuntze) and Characterization of Amino Acid Transporter <i>CsCAT9.1</i> |
| title_sort | transcriptome wide analysis of nitrogen regulated genes in tea plant i camellia sinensis i l o kuntze and characterization of amino acid transporter i cscat9 1 i |
| topic | assimilation <i>Camellia sinensis</i> nitrogen transport transcriptome |
| url | https://www.mdpi.com/2223-7747/9/9/1218 |
| work_keys_str_mv | AT xinwanzhang transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT honglingliu transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT elizabethpilonsmits transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT weihuang transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT puwang transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT minglewang transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT feiguo transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT yuwang transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT ruiyuanli transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT huazhao transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i AT dejiangni transcriptomewideanalysisofnitrogenregulatedgenesinteaplanticamelliasinensisilokuntzeandcharacterizationofaminoacidtransportericscat91i |