Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement
This study developed a method to simultaneously determine 73 multi-class pesticides in okra fruit using LC-MS/MS and GC–MS/MS. The sample was extracted with acetonitrile and subsequent clean-up through dispersive-SPE method. The quantification level of the technique was 0.01 µg g−1 and compliance to...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-10-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157523002572 |
| _version_ | 1797676534249553920 |
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| author | M.S. Pallavi R. Harischandra Naik K. Pavankumar Ratnamma Nandini A. Shwetha P. Naveenkumar M. Paramasivam R. Udaykumar Nidoni A. Prabhuraj M. Bheemanna |
| author_facet | M.S. Pallavi R. Harischandra Naik K. Pavankumar Ratnamma Nandini A. Shwetha P. Naveenkumar M. Paramasivam R. Udaykumar Nidoni A. Prabhuraj M. Bheemanna |
| author_sort | M.S. Pallavi |
| collection | DOAJ |
| description | This study developed a method to simultaneously determine 73 multi-class pesticides in okra fruit using LC-MS/MS and GC–MS/MS. The sample was extracted with acetonitrile and subsequent clean-up through dispersive-SPE method. The quantification level of the technique was 0.01 µg g−1 and compliance to the MRLs fixed by the regulatory bodies like EU and FSSAI. The recovery at 10, 50, and 100 µg kg−1 spiked levels; intra and inter-day precision at 50 µg kg−1 were found within 70–120% with RSD less than 15% with LC-MS/MS and GC–MS/MS. Measurement uncertainty was in the range of 1.81 to 12.91 µg kg−1 estimated at 50 µg kg−1. The matrix effects were slightly higher for LC than GC-compatible pesticides. Risk assessment for pesticides detected in the field and market samples found no hazardous to the consumers except profenofos. The proposed method is highly sensitive, reproducible for the complex matrix like okra, and meets the regulatory standards. |
| first_indexed | 2024-03-11T22:30:34Z |
| format | Article |
| id | doaj.art-1d8023994dc14208a7b4e3e9b8cdd576 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-03-11T22:30:34Z |
| publishDate | 2023-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-1d8023994dc14208a7b4e3e9b8cdd5762023-09-23T05:12:22ZengElsevierFood Chemistry: X2590-15752023-10-0119100814Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurementM.S. Pallavi0R. Harischandra Naik1K. Pavankumar2 Ratnamma3 Nandini4A. Shwetha5P. Naveenkumar6M. Paramasivam7R. Udaykumar Nidoni8A. Prabhuraj9M. Bheemanna10University of Agricultural Sciences, Raichur 584 104, India; KSN University of Agricultural and Horticultural Sciences, Shivamogga 577 412, IndiaUniversity of Agricultural Sciences, Raichur 584 104, India; University of Horticultural Sciences, Bagalkot, College of Horticulture, Bangalore 560 065, Karnataka, India; Corresponding author at: University of Horticultural Sciences, Bagalkot, India; University of Agricultural Sciences Raichur, India.University of Agricultural Sciences, Raichur 584 104, IndiaUniversity of Agricultural Sciences, Raichur 584 104, IndiaUniversity of Agricultural Sciences, Raichur 584 104, IndiaUniversity of Agricultural Sciences, Raichur 584 104, IndiaUniversity of Agricultural Sciences, Raichur 584 104, IndiaTamil Nadu Agricultural University, Coimbatore 641 003, IndiaUniversity of Agricultural Sciences, Raichur 584 104, IndiaUniversity of Agricultural Sciences, Raichur 584 104, IndiaUniversity of Agricultural Sciences, Raichur 584 104, IndiaThis study developed a method to simultaneously determine 73 multi-class pesticides in okra fruit using LC-MS/MS and GC–MS/MS. The sample was extracted with acetonitrile and subsequent clean-up through dispersive-SPE method. The quantification level of the technique was 0.01 µg g−1 and compliance to the MRLs fixed by the regulatory bodies like EU and FSSAI. The recovery at 10, 50, and 100 µg kg−1 spiked levels; intra and inter-day precision at 50 µg kg−1 were found within 70–120% with RSD less than 15% with LC-MS/MS and GC–MS/MS. Measurement uncertainty was in the range of 1.81 to 12.91 µg kg−1 estimated at 50 µg kg−1. The matrix effects were slightly higher for LC than GC-compatible pesticides. Risk assessment for pesticides detected in the field and market samples found no hazardous to the consumers except profenofos. The proposed method is highly sensitive, reproducible for the complex matrix like okra, and meets the regulatory standards.http://www.sciencedirect.com/science/article/pii/S2590157523002572Okra fruitsPesticidesQuEChERSLC-MS/MSGC–MS/MSRisk assessment |
| spellingShingle | M.S. Pallavi R. Harischandra Naik K. Pavankumar Ratnamma Nandini A. Shwetha P. Naveenkumar M. Paramasivam R. Udaykumar Nidoni A. Prabhuraj M. Bheemanna Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement Food Chemistry: X Okra fruits Pesticides QuEChERS LC-MS/MS GC–MS/MS Risk assessment |
| title | Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement |
| title_full | Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement |
| title_fullStr | Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement |
| title_full_unstemmed | Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement |
| title_short | Determination of 73 multi-class pesticides in okra (Abelmoschus esculentus L.) fruits using LC–MS/MS and GC–MS/MS and estimation of analytical uncertainty of measurement |
| title_sort | determination of 73 multi class pesticides in okra abelmoschus esculentus l fruits using lc ms ms and gc ms ms and estimation of analytical uncertainty of measurement |
| topic | Okra fruits Pesticides QuEChERS LC-MS/MS GC–MS/MS Risk assessment |
| url | http://www.sciencedirect.com/science/article/pii/S2590157523002572 |
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