Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources
Three novel hydrogen-generating strains, ST1, ST4, and ST5, were isolated from the rumen of cow in Vietnam, and respectively identified as <em>Clostridium beijerinckii</em> ST1, <em>Clostridium bifermentans</em> ST4, and <em>Clostridium butyricum</em> ST5, based o...
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| Format: | Article |
| Language: | English |
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AIMS Press
2018-10-01
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| Series: | AIMS Energy |
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| Online Access: | http://www.aimspress.com/energy/article/3131/fulltext.html |
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| author | Vui Thi Hoang Diem Huong Hoang Ngoc Duc Pham Hanh My Tran Ha Thi Viet Bui Tien Anh Ngo |
| author_facet | Vui Thi Hoang Diem Huong Hoang Ngoc Duc Pham Hanh My Tran Ha Thi Viet Bui Tien Anh Ngo |
| author_sort | Vui Thi Hoang |
| collection | DOAJ |
| description | Three novel hydrogen-generating strains, ST1, ST4, and ST5, were isolated from the rumen of cow in Vietnam, and respectively identified as <em>Clostridium beijerinckii</em> ST1, <em>Clostridium bifermentans</em> ST4, and <em>Clostridium butyricum</em> ST5, based on 16S rDNA gene sequence analysis and physiobiochemical characteristics. The dark fermentative hydrogen production of these isolated <em>Clostridium</em> strains was performed and characterized in both pure- and co-cultures from various carbon sources including sucrose, glucose, lactose, xylose, molasses, cassava stumps, and rice distillers wet grains with soluble. The highest hydrogen production was achieved from a co-culture with three <em>Clostridium</em> strains. To optimize the operational conditions of temperature, time, and substrate concentration for the high-level production of hydrogen, response surface methodology in a Box-Behnken design was used. The results revealed a maximum hydrogen production of 1.13 ± 0.015 L H<sub>2</sub>/L medium by the three-strain co-culture under the following fermentation conditions: 11.63 g/L sucrose, 36.1 °C, in 51.13 h. |
| first_indexed | 2024-12-11T19:57:31Z |
| format | Article |
| id | doaj.art-1d8170edc13349b2b799f23f1dfb77b5 |
| institution | Directory Open Access Journal |
| issn | 2333-8334 |
| language | English |
| last_indexed | 2024-12-11T19:57:31Z |
| publishDate | 2018-10-01 |
| publisher | AIMS Press |
| record_format | Article |
| series | AIMS Energy |
| spelling | doaj.art-1d8170edc13349b2b799f23f1dfb77b52022-12-22T00:52:36ZengAIMS PressAIMS Energy2333-83342018-10-016584686510.3934/energy.2018.5.846energy-06-05-846Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sourcesVui Thi Hoang0Diem Huong Hoang1Ngoc Duc Pham2Hanh My Tran3Ha Thi Viet Bui4Tien Anh NgoDepartment of Microbiology, Hanoi University of Science, Vietnam National University, Hanoi, VietnamDepartment of Microbiology, Hanoi University of Science, Vietnam National University, Hanoi, VietnamDepartment of Microbiology, Hanoi University of Science, Vietnam National University, Hanoi, VietnamDepartment of Microbiology, Hanoi University of Science, Vietnam National University, Hanoi, VietnamDepartment of Microbiology, Hanoi University of Science, Vietnam National University, Hanoi, VietnamThree novel hydrogen-generating strains, ST1, ST4, and ST5, were isolated from the rumen of cow in Vietnam, and respectively identified as <em>Clostridium beijerinckii</em> ST1, <em>Clostridium bifermentans</em> ST4, and <em>Clostridium butyricum</em> ST5, based on 16S rDNA gene sequence analysis and physiobiochemical characteristics. The dark fermentative hydrogen production of these isolated <em>Clostridium</em> strains was performed and characterized in both pure- and co-cultures from various carbon sources including sucrose, glucose, lactose, xylose, molasses, cassava stumps, and rice distillers wet grains with soluble. The highest hydrogen production was achieved from a co-culture with three <em>Clostridium</em> strains. To optimize the operational conditions of temperature, time, and substrate concentration for the high-level production of hydrogen, response surface methodology in a Box-Behnken design was used. The results revealed a maximum hydrogen production of 1.13 ± 0.015 L H<sub>2</sub>/L medium by the three-strain co-culture under the following fermentation conditions: 11.63 g/L sucrose, 36.1 °C, in 51.13 h.http://www.aimspress.com/energy/article/3131/fulltext.htmlhydrogen productionClostridiumco-culturecow rumen bacteriaresponse surface methodologyfood industry wastes |
| spellingShingle | Vui Thi Hoang Diem Huong Hoang Ngoc Duc Pham Hanh My Tran Ha Thi Viet Bui Tien Anh Ngo Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources AIMS Energy hydrogen production Clostridium co-culture cow rumen bacteria response surface methodology food industry wastes |
| title | Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources |
| title_full | Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources |
| title_fullStr | Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources |
| title_full_unstemmed | Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources |
| title_short | Hydrogen production by newly isolated <em>Clostridium</em> species from cow rumen in pure- and co-cultures on a broad range of carbon sources |
| title_sort | hydrogen production by newly isolated em clostridium em species from cow rumen in pure and co cultures on a broad range of carbon sources |
| topic | hydrogen production Clostridium co-culture cow rumen bacteria response surface methodology food industry wastes |
| url | http://www.aimspress.com/energy/article/3131/fulltext.html |
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