Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses
The calmodulin-binding transcription activators (CAMTAs) mediate transcriptional regulation of development, growth, and responses to various environmental stresses in plants. To understand the biological roles of soybean <i>CAMTA</i> (<i>GmCAMTA</i>) family members in respons...
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MDPI AG
2023-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/14/11477 |
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| author | Dongwon Baek Hyun Min Cho Ye Jin Cha Byung Jun Jin Su Hyeon Lee Mi Suk Park Hyun Jin Chun Min Chul Kim |
| author_facet | Dongwon Baek Hyun Min Cho Ye Jin Cha Byung Jun Jin Su Hyeon Lee Mi Suk Park Hyun Jin Chun Min Chul Kim |
| author_sort | Dongwon Baek |
| collection | DOAJ |
| description | The calmodulin-binding transcription activators (CAMTAs) mediate transcriptional regulation of development, growth, and responses to various environmental stresses in plants. To understand the biological roles of soybean <i>CAMTA</i> (<i>GmCAMTA</i>) family members in response to abiotic stresses, we characterized expression patterns of 15 <i>GmCAMTA</i> genes in response to various abiotic stresses. The <i>GmCAMTA</i> genes exhibited distinct circadian regulation expression patterns and were differently expressed in response to salt, drought, and cold stresses. Interestingly, the expression levels of <i>GmCAMTA2</i>, <i>GmCAMTA8</i>, and <i>GmCAMTA12</i> were higher in stem tissue than in other soybean tissues. To determine the roles of <i>GmCAMTAs</i> in the regulation of developmental processes and stress responses, we isolated <i>GmCAMTA2</i> and <i>GmCAMTA8</i> cDNAs from soybean and generated Arabidopsis overexpressing transgenic plants. The <i>GmCAMTA2</i>-OX and <i>GmCAMTA8</i>-OX plants showed hypersensitivity to drought stress. The water in the leaves of <i>GmCAMTA2</i>-OX and <i>GmCAMTA8</i>-OX plants was lost faster than that in wild-type (WT) plants under drought-stress conditions. In addition, stress-responsive genes were down-regulated in the <i>GmCAMTA2</i>-OX and <i>GmCAMTA8</i>-OX plants under drought stress conditions compared to WT plants. Our results suggest that <i>GmCAMTA2</i> and <i>GmCAMTA8</i> genes are regulated by circadian rhythms and function as negative regulators in development and drought stress responses. |
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| language | English |
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| publishDate | 2023-07-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1d85782af9264a5aae812b452b2cbfb62023-11-18T19:40:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-07-0124141147710.3390/ijms241411477Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress ResponsesDongwon Baek0Hyun Min Cho1Ye Jin Cha2Byung Jun Jin3Su Hyeon Lee4Mi Suk Park5Hyun Jin Chun6Min Chul Kim7Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of KoreaInstitute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaDivision of Applied Life Science (BK21 Four), Gyeongsang National University, Jinju 52828, Republic of KoreaPlant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Republic of KoreaInstitute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of KoreaPlant Molecular Biology and Biotechnology Research Center, Gyeongsang National University, Jinju 52828, Republic of KoreaThe calmodulin-binding transcription activators (CAMTAs) mediate transcriptional regulation of development, growth, and responses to various environmental stresses in plants. To understand the biological roles of soybean <i>CAMTA</i> (<i>GmCAMTA</i>) family members in response to abiotic stresses, we characterized expression patterns of 15 <i>GmCAMTA</i> genes in response to various abiotic stresses. The <i>GmCAMTA</i> genes exhibited distinct circadian regulation expression patterns and were differently expressed in response to salt, drought, and cold stresses. Interestingly, the expression levels of <i>GmCAMTA2</i>, <i>GmCAMTA8</i>, and <i>GmCAMTA12</i> were higher in stem tissue than in other soybean tissues. To determine the roles of <i>GmCAMTAs</i> in the regulation of developmental processes and stress responses, we isolated <i>GmCAMTA2</i> and <i>GmCAMTA8</i> cDNAs from soybean and generated Arabidopsis overexpressing transgenic plants. The <i>GmCAMTA2</i>-OX and <i>GmCAMTA8</i>-OX plants showed hypersensitivity to drought stress. The water in the leaves of <i>GmCAMTA2</i>-OX and <i>GmCAMTA8</i>-OX plants was lost faster than that in wild-type (WT) plants under drought-stress conditions. In addition, stress-responsive genes were down-regulated in the <i>GmCAMTA2</i>-OX and <i>GmCAMTA8</i>-OX plants under drought stress conditions compared to WT plants. Our results suggest that <i>GmCAMTA2</i> and <i>GmCAMTA8</i> genes are regulated by circadian rhythms and function as negative regulators in development and drought stress responses.https://www.mdpi.com/1422-0067/24/14/11477GmCAMTAcircadian rhythmdevelopmentdrought stress |
| spellingShingle | Dongwon Baek Hyun Min Cho Ye Jin Cha Byung Jun Jin Su Hyeon Lee Mi Suk Park Hyun Jin Chun Min Chul Kim Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses International Journal of Molecular Sciences GmCAMTA circadian rhythm development drought stress |
| title | Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses |
| title_full | Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses |
| title_fullStr | Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses |
| title_full_unstemmed | Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses |
| title_short | Soybean Calmodulin-Binding Transcription Activators, GmCAMTA2 and GmCAMTA8, Coordinate the Circadian Regulation of Developmental Processes and Drought Stress Responses |
| title_sort | soybean calmodulin binding transcription activators gmcamta2 and gmcamta8 coordinate the circadian regulation of developmental processes and drought stress responses |
| topic | GmCAMTA circadian rhythm development drought stress |
| url | https://www.mdpi.com/1422-0067/24/14/11477 |
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