Effect of Oral Morphine Consumption in Female Rats on Development of Brain Cavities, Central Canal and Choroid Plexus of Their Embryos
Objective: Previous studies have shown that morphine consumption during pregnancymay delay embryo development or cause abnormal nervous system function. The presentstudy focused on the effects of maternal morphine consumption on brain cavities andcentral canal development in Wistar rats.Materials an...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2011-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | http://celljournal.org/library/upload/article/af_4233632Kazemi.pdf |
| Summary: | Objective: Previous studies have shown that morphine consumption during pregnancymay delay embryo development or cause abnormal nervous system function. The presentstudy focused on the effects of maternal morphine consumption on brain cavities andcentral canal development in Wistar rats.Materials and Methods: In this study Wistar rats (average weight: 170-200 g) were used.The experimental group, after pregnancy, received 0.05 mg/ml of morphine by tap waterwhile the control group received water. On the 17th day of pregnancy, the pregnant animalswere anesthetized by chloroform and embryos were surgically removed. The sampleswere fixed in 10% formalin for four weeks. Then, tissues were processed and sectioned.Sections were stained with hematoxylin and eosin (H&E) and examined for ventricle, centralcanal and choroid plexus development by light microscopy and MOTIC software.Results: Severe reductions of the third and lateral ventricles were observed in the experimentalgroup. In addition, an increase in the choroid plexus (CP) area in the experimentalgroup with regards to the control group was identified.Conclusion: The study showed that oral morphine consumption lead to reduction in thethird and lateral brain cavities and an increase in the CP area. This defect may cause behavioralchanges observed in the F1 generation from addicted pregnant animals. |
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| ISSN: | 2228-5806 2228-5814 |