In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation
Fast-growing forest species with multiple uses, like bamboo, have aroused interest for their silvicultural applications. Bamboo species are a valuable source of renewable raw material, and Bambusa vulgaris is an economically important species. However, there are limitations to large-scale cloning of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-05-01
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| Series: | Advances in Bamboo Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773139123000101 |
| _version_ | 1797802019404120064 |
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| author | Douglas Santos Gonçalves Denys Matheus Santana Costa Souza Dulcinéia de Carvalho Leandro Silva de Oliveira Gustavo Leal Teixeira Gilvano Ebling Brondani |
| author_facet | Douglas Santos Gonçalves Denys Matheus Santana Costa Souza Dulcinéia de Carvalho Leandro Silva de Oliveira Gustavo Leal Teixeira Gilvano Ebling Brondani |
| author_sort | Douglas Santos Gonçalves |
| collection | DOAJ |
| description | Fast-growing forest species with multiple uses, like bamboo, have aroused interest for their silvicultural applications. Bamboo species are a valuable source of renewable raw material, and Bambusa vulgaris is an economically important species. However, there are limitations to large-scale cloning of adult-selected genotypes. This study aimed to evaluate the in vitro cloning of Bambusa vulgaris in different culture systems, sucrose and activated charcoal supplementation by the inter-simple-sequence repeat (ISSR) molecular markers. In vitro bud multiplication and shoot elongation were evaluated in three cultivation systems: semi-solid and liquid culture media, and temporary immersion bioreactor (TIB). The sucrose concentrations, 0 and 30 g L−1 were evaluated in the stages. Both the culture media were supplemented with 2.0 mg L−1 benzylaminopurine (BAP) and 0.5 mg L−1 α-naphthalene acetic acid (NAA). The absence and presence of activated charcoal (100 mg L−1) were evaluated in the in vitro rooting. MS culture medium was supplemented with 2 mg L−1 indole-3-butyric acid (IBA), 1.0 mg L−1 NAA, and 0.5 mg L−1 BAP. Semi-solid culture medium supplemented with 30 g L−1 of sucrose presented superior emission of bud per explant. Liquid culture medium supplemented with 30 g L−1 of sucrose presented the most elongated shoots. Activated charcoal in the culture medium did not influence the adventitious rooting. Micropropagated plants showed genetic fidelity and were clones of the adult selected plant. |
| first_indexed | 2024-03-13T04:59:23Z |
| format | Article |
| id | doaj.art-1d85cccc28d94246aa6785decbd8e4ec |
| institution | Directory Open Access Journal |
| issn | 2773-1391 |
| language | English |
| last_indexed | 2024-03-13T04:59:23Z |
| publishDate | 2023-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Advances in Bamboo Science |
| spelling | doaj.art-1d85cccc28d94246aa6785decbd8e4ec2023-06-17T05:21:59ZengElsevierAdvances in Bamboo Science2773-13912023-05-013100024In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementationDouglas Santos Gonçalves0Denys Matheus Santana Costa Souza1Dulcinéia de Carvalho2Leandro Silva de Oliveira3Gustavo Leal Teixeira4Gilvano Ebling Brondani5Laboratory of In Vitro Culture of Forest Species, Department of Forestry Sciences, Federal University of Lavras, University Campus, Po. Box. 3037, 37200-900 Lavras, Minas Gerais, BrazilLaboratory of In Vitro Culture of Forest Species, Department of Forestry Sciences, Federal University of Lavras, University Campus, Po. Box. 3037, 37200-900 Lavras, Minas Gerais, BrazilLaboratory of In Vitro Culture of Forest Species, Department of Forestry Sciences, Federal University of Lavras, University Campus, Po. Box. 3037, 37200-900 Lavras, Minas Gerais, BrazilInstitute of Agricultural Science, Federal University of Minas Gerais, University Campus, 39404-547 Montes Claros, Minas Gerais, BrazilInstitute of Agricultural Science, Federal University of Minas Gerais, University Campus, 39404-547 Montes Claros, Minas Gerais, BrazilLaboratory of In Vitro Culture of Forest Species, Department of Forestry Sciences, Federal University of Lavras, University Campus, Po. Box. 3037, 37200-900 Lavras, Minas Gerais, Brazil; Corresponding author.Fast-growing forest species with multiple uses, like bamboo, have aroused interest for their silvicultural applications. Bamboo species are a valuable source of renewable raw material, and Bambusa vulgaris is an economically important species. However, there are limitations to large-scale cloning of adult-selected genotypes. This study aimed to evaluate the in vitro cloning of Bambusa vulgaris in different culture systems, sucrose and activated charcoal supplementation by the inter-simple-sequence repeat (ISSR) molecular markers. In vitro bud multiplication and shoot elongation were evaluated in three cultivation systems: semi-solid and liquid culture media, and temporary immersion bioreactor (TIB). The sucrose concentrations, 0 and 30 g L−1 were evaluated in the stages. Both the culture media were supplemented with 2.0 mg L−1 benzylaminopurine (BAP) and 0.5 mg L−1 α-naphthalene acetic acid (NAA). The absence and presence of activated charcoal (100 mg L−1) were evaluated in the in vitro rooting. MS culture medium was supplemented with 2 mg L−1 indole-3-butyric acid (IBA), 1.0 mg L−1 NAA, and 0.5 mg L−1 BAP. Semi-solid culture medium supplemented with 30 g L−1 of sucrose presented superior emission of bud per explant. Liquid culture medium supplemented with 30 g L−1 of sucrose presented the most elongated shoots. Activated charcoal in the culture medium did not influence the adventitious rooting. Micropropagated plants showed genetic fidelity and were clones of the adult selected plant.http://www.sciencedirect.com/science/article/pii/S2773139123000101BambooMicropropagationGenetic fidelityISSRTemporary immersion bioreactor |
| spellingShingle | Douglas Santos Gonçalves Denys Matheus Santana Costa Souza Dulcinéia de Carvalho Leandro Silva de Oliveira Gustavo Leal Teixeira Gilvano Ebling Brondani In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation Advances in Bamboo Science Bamboo Micropropagation Genetic fidelity ISSR Temporary immersion bioreactor |
| title | In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation |
| title_full | In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation |
| title_fullStr | In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation |
| title_full_unstemmed | In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation |
| title_short | In vitro cloning of Bambusa vulgaris Schrad. ex J. C. Wendl.: Effect of culture systems, sucrose and activated charcoal supplementation |
| title_sort | in vitro cloning of bambusa vulgaris schrad ex j c wendl effect of culture systems sucrose and activated charcoal supplementation |
| topic | Bamboo Micropropagation Genetic fidelity ISSR Temporary immersion bioreactor |
| url | http://www.sciencedirect.com/science/article/pii/S2773139123000101 |
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