Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar rats
Objective(s): The objectives of the current study were to evaluate the effects of hepatic ischemia/reperfusion (IR) injury on the activity of antioxidant enzymes, biochemical factors, and histopathological changes in rat kidney, and to investigate the effect of crocin on IR-related changes. Mater...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2017-10-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_9450_a41563b5edf2f136da8a804c2e759d1a.pdf |
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| author | Seyyed Ali Mard Ghaidafeh Akbari Esrafil Mansouri Mahdi Parsanahad |
| author_facet | Seyyed Ali Mard Ghaidafeh Akbari Esrafil Mansouri Mahdi Parsanahad |
| author_sort | Seyyed Ali Mard |
| collection | DOAJ |
| description | Objective(s): The objectives of the current study were to evaluate the effects of hepatic ischemia/reperfusion (IR) injury on the activity of antioxidant enzymes, biochemical factors, and histopathological changes in rat kidney, and to investigate the effect of crocin on IR-related changes. Materials and Methods: Thirty-two male Wistar rats were randomly allocated into four groups (n=8). They were sham-operated, IR, crocin pre-treatment, and crocin pretreatment+IR groups. Sham-operated and Crocin pre-treatment groups received normal saline (N/S, 2 ml/day) and crocin (200 mg/kg) for seven consecutive days intraperitoneally (IP), respectively, then rats underwent laparotomy, only. IR and crocin pretreatment+IR groups received N/S and crocin with the same dose, time, and route, respectively, then rats underwent partial (70%) ischemia for 45 min that was followed by reperfusion for 60 min. At the end of the experiment, kidney specimens were taken for histopathological and antioxidant evaluations and also blood samples were obtained for biochemical analysis. Results: The results of the present study showed that crocin pre-treatment significantly increased the activity of antioxidants, decreased the serum levels of liver enzymes and blood urea nitrogen following IR-induced hepatic injury. Crocin also ameliorated kidney´s histopathological disturbance beyond IR-induced hepatic injury. Conclusion: Crocin as an antioxidant agent protected renal insult following liver IR injury by increasing the activity of antioxidant enzymes, reducing serum levels of liver enzymes, and improving histopathological changes. |
| first_indexed | 2024-12-18T19:15:23Z |
| format | Article |
| id | doaj.art-1d86103a977d48d48cab5f296ff0ff79 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-18T19:15:23Z |
| publishDate | 2017-10-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-1d86103a977d48d48cab5f296ff0ff792022-12-21T20:56:09ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742017-10-0120101172117710.22038/ijbms.2017.94509450Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar ratsSeyyed Ali Mard0Ghaidafeh Akbari1Esrafil Mansouri2Mahdi Parsanahad3Research Center for Infectious Diseases of Digestive System (Alimentary Tract Research Center), Physiology Research Center (PRC), Dept. of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranResearch Center for Infectious Diseases of Digestive System (Alimentary Tract Research Center), Physiology Research Center (PRC), Dept. of Physiology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranCellular and Molecular Research Center, Department of Anatomic Sciences, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranVirology department, School of Medicine Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranObjective(s): The objectives of the current study were to evaluate the effects of hepatic ischemia/reperfusion (IR) injury on the activity of antioxidant enzymes, biochemical factors, and histopathological changes in rat kidney, and to investigate the effect of crocin on IR-related changes. Materials and Methods: Thirty-two male Wistar rats were randomly allocated into four groups (n=8). They were sham-operated, IR, crocin pre-treatment, and crocin pretreatment+IR groups. Sham-operated and Crocin pre-treatment groups received normal saline (N/S, 2 ml/day) and crocin (200 mg/kg) for seven consecutive days intraperitoneally (IP), respectively, then rats underwent laparotomy, only. IR and crocin pretreatment+IR groups received N/S and crocin with the same dose, time, and route, respectively, then rats underwent partial (70%) ischemia for 45 min that was followed by reperfusion for 60 min. At the end of the experiment, kidney specimens were taken for histopathological and antioxidant evaluations and also blood samples were obtained for biochemical analysis. Results: The results of the present study showed that crocin pre-treatment significantly increased the activity of antioxidants, decreased the serum levels of liver enzymes and blood urea nitrogen following IR-induced hepatic injury. Crocin also ameliorated kidney´s histopathological disturbance beyond IR-induced hepatic injury. Conclusion: Crocin as an antioxidant agent protected renal insult following liver IR injury by increasing the activity of antioxidant enzymes, reducing serum levels of liver enzymes, and improving histopathological changes.http://ijbms.mums.ac.ir/article_9450_a41563b5edf2f136da8a804c2e759d1a.pdfAntioxidantCrocinIschemia/reperfusion KidneyLiverRat |
| spellingShingle | Seyyed Ali Mard Ghaidafeh Akbari Esrafil Mansouri Mahdi Parsanahad Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar rats Iranian Journal of Basic Medical Sciences Antioxidant Crocin Ischemia/reperfusion Kidney Liver Rat |
| title | Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar rats |
| title_full | Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar rats |
| title_fullStr | Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar rats |
| title_full_unstemmed | Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar rats |
| title_short | Renoprotective effect of crocin following liver ischemia/ reperfusion injury in Wistar rats |
| title_sort | renoprotective effect of crocin following liver ischemia reperfusion injury in wistar rats |
| topic | Antioxidant Crocin Ischemia/reperfusion Kidney Liver Rat |
| url | http://ijbms.mums.ac.ir/article_9450_a41563b5edf2f136da8a804c2e759d1a.pdf |
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