Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomas
Hematogones are precursors of B-lymphocytes detected in small numbers in the bone marrow. Flow cytometry is the most useful tool to identify hematogones and, so far, 4-color methods have been published. In addition, flow cytometry is used in the diagnosis and follow-up of lymphomas. We developed a f...
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MDPI AG
2014-06-01
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| Series: | Hematology Reports |
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| Online Access: | http://www.pagepress.org/journals/index.php/hr/article/view/5381 |
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| author | Giovanni Carulli Virginia Ottaviano Valentina Guerri Stefano Giuntini Paola Sammuri Eugenio M. Ciancia Antonio Azzarà |
| author_facet | Giovanni Carulli Virginia Ottaviano Valentina Guerri Stefano Giuntini Paola Sammuri Eugenio M. Ciancia Antonio Azzarà |
| author_sort | Giovanni Carulli |
| collection | DOAJ |
| description | Hematogones are precursors of B-lymphocytes detected in small numbers in the bone marrow. Flow cytometry is the most useful tool to identify hematogones and, so far, 4-color methods have been published. In addition, flow cytometry is used in the diagnosis and follow-up of lymphomas. We developed a flow cytometric 7-color method to enumerate hematogones and to assess B-lymphocyte clonality for routine purposes. We evaluated 171 cases of B-cell non-Hodgkin lymphomas, either at diagnosis or in the course of follow-up. By our diagnostic method, which was carried out by the combination K/l/CD20/CD19/CD10/CD45/CD5, we were able to detect hematogones in 97.6% of samples and to distinguish normal B-lymphocytes, neoplastic lymphocytes and hematogones in a single step. The percentage of hematogones showed a significant inverse correlation with the degree of neoplastic infiltration and, when bone marrow samples not involved by disease were taken into consideration, resulted higher in patients during follow-up than in patients evaluated at diagnosis. |
| first_indexed | 2024-04-10T18:36:01Z |
| format | Article |
| id | doaj.art-1d8a0bac019f421b98f842878b662e23 |
| institution | Directory Open Access Journal |
| issn | 2038-8322 2038-8330 |
| language | English |
| last_indexed | 2024-04-10T18:36:01Z |
| publishDate | 2014-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Hematology Reports |
| spelling | doaj.art-1d8a0bac019f421b98f842878b662e232023-02-02T01:22:12ZengMDPI AGHematology Reports2038-83222038-83302014-06-016210.4081/hr.2014.53812869Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomasGiovanni Carulli0Virginia Ottaviano1Valentina Guerri2Stefano Giuntini3Paola Sammuri4Eugenio M. Ciancia5Antonio Azzarà6Division of Hematology, Department of Clinical and Experimental Medicine, Santa Chiara Hospital, University of PisaDivision of Hematology, Department of Clinical and Experimental Medicine, Santa Chiara Hospital, University of PisaDivision of Hematology, Department of Clinical and Experimental Medicine, Santa Chiara Hospital, University of PisaDivision of Hematology, Department of Clinical and Experimental Medicine, Santa Chiara Hospital, University of PisaDivision of Hematology, Department of Clinical and Experimental Medicine, Santa Chiara Hospital, University of PisaSecond Division of Pathology, Pisa University HospitalDivision of Hematology, Department of Clinical and Experimental Medicine, Santa Chiara Hospital, University of PisaHematogones are precursors of B-lymphocytes detected in small numbers in the bone marrow. Flow cytometry is the most useful tool to identify hematogones and, so far, 4-color methods have been published. In addition, flow cytometry is used in the diagnosis and follow-up of lymphomas. We developed a flow cytometric 7-color method to enumerate hematogones and to assess B-lymphocyte clonality for routine purposes. We evaluated 171 cases of B-cell non-Hodgkin lymphomas, either at diagnosis or in the course of follow-up. By our diagnostic method, which was carried out by the combination K/l/CD20/CD19/CD10/CD45/CD5, we were able to detect hematogones in 97.6% of samples and to distinguish normal B-lymphocytes, neoplastic lymphocytes and hematogones in a single step. The percentage of hematogones showed a significant inverse correlation with the degree of neoplastic infiltration and, when bone marrow samples not involved by disease were taken into consideration, resulted higher in patients during follow-up than in patients evaluated at diagnosis.http://www.pagepress.org/journals/index.php/hr/article/view/5381B-lymphocytes, bone marrow, hematogones, multiparameter flow cytometry, non-Hodgkin lymphomas |
| spellingShingle | Giovanni Carulli Virginia Ottaviano Valentina Guerri Stefano Giuntini Paola Sammuri Eugenio M. Ciancia Antonio Azzarà Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomas Hematology Reports B-lymphocytes, bone marrow, hematogones, multiparameter flow cytometry, non-Hodgkin lymphomas |
| title | Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomas |
| title_full | Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomas |
| title_fullStr | Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomas |
| title_full_unstemmed | Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomas |
| title_short | Multiparameter flow cytometry to detect hematogones and to assess B-lymphocyte clonality in bone marrow samples from patients with non-Hodgkin lymphomas |
| title_sort | multiparameter flow cytometry to detect hematogones and to assess b lymphocyte clonality in bone marrow samples from patients with non hodgkin lymphomas |
| topic | B-lymphocytes, bone marrow, hematogones, multiparameter flow cytometry, non-Hodgkin lymphomas |
| url | http://www.pagepress.org/journals/index.php/hr/article/view/5381 |
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