Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice
Liver health has long been linked to the homeostasis of gut microbiota. Although some studies have shown that alterations in the species and function of gut microbiota contribute to the initiation and development of acute liver injury (ALI), studies investigating the effects of ALI on gut microbial...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Medicine in Microecology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590097822000131 |
| _version_ | 1811257545975660544 |
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| author | Fengyi Mei Tao Chen Xianglong Zhang Peng Chen |
| author_facet | Fengyi Mei Tao Chen Xianglong Zhang Peng Chen |
| author_sort | Fengyi Mei |
| collection | DOAJ |
| description | Liver health has long been linked to the homeostasis of gut microbiota. Although some studies have shown that alterations in the species and function of gut microbiota contribute to the initiation and development of acute liver injury (ALI), studies investigating the effects of ALI on gut microbial dynamic composition changes are still limited. To observe whether liver damage can alter the composition of gut microbiota dynamically, we established three chemical models (e.g., acetaminophen, carbon tetrachloride, lipopolysaccharide) of ALI. Using these models, multiple time points of liver injury and intestinal microbiome were analyzed through plasma biochemical analysis and 16S rRNA gene sequencing. We assessed α-diversity, Unifrac principal coordinates analysis (PCoA), and linear discriminant analysis effect size (LEfSe) in the injury and control groups. The composition of the gut microbiota underwent dramatic shifts with liver injury and recovery in each model. Additionally, specific microbial abundance was significantly correlated with the level of plasma alanine transaminase and aspartate transaminase. These data provide new evidence that liver dysfunction and restoration is dynamically linked with the changes in the intestinal microbiome. |
| first_indexed | 2024-04-12T17:59:05Z |
| format | Article |
| id | doaj.art-1d8a1f64c4014dbeaf09cd937006826f |
| institution | Directory Open Access Journal |
| issn | 2590-0978 |
| language | English |
| last_indexed | 2024-04-12T17:59:05Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Medicine in Microecology |
| spelling | doaj.art-1d8a1f64c4014dbeaf09cd937006826f2022-12-22T03:22:13ZengElsevierMedicine in Microecology2590-09782022-12-0114100063Acute liver injury progression is associated with dynamic enteric eubiosis alteration in miceFengyi Mei0Tao Chen1Xianglong Zhang2Peng Chen3Department of Pathophysiology, Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDepartment of Pathophysiology, Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDepartment of Pathophysiology, Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, ChinaDepartment of Pathophysiology, Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China; Microbiome Medicine Center, Zhujiang Hospital, Southern Medical University, Guangzhou, China; Corresponding author. Department of Pathophysiology, Guangdong Provincial Key Laboratory of Proteomics, School of Basic Medical Sciences, Southern Medical University, Guangzhou, China.Liver health has long been linked to the homeostasis of gut microbiota. Although some studies have shown that alterations in the species and function of gut microbiota contribute to the initiation and development of acute liver injury (ALI), studies investigating the effects of ALI on gut microbial dynamic composition changes are still limited. To observe whether liver damage can alter the composition of gut microbiota dynamically, we established three chemical models (e.g., acetaminophen, carbon tetrachloride, lipopolysaccharide) of ALI. Using these models, multiple time points of liver injury and intestinal microbiome were analyzed through plasma biochemical analysis and 16S rRNA gene sequencing. We assessed α-diversity, Unifrac principal coordinates analysis (PCoA), and linear discriminant analysis effect size (LEfSe) in the injury and control groups. The composition of the gut microbiota underwent dramatic shifts with liver injury and recovery in each model. Additionally, specific microbial abundance was significantly correlated with the level of plasma alanine transaminase and aspartate transaminase. These data provide new evidence that liver dysfunction and restoration is dynamically linked with the changes in the intestinal microbiome.http://www.sciencedirect.com/science/article/pii/S2590097822000131Acute liver injuryGut microbiota16S rRNA |
| spellingShingle | Fengyi Mei Tao Chen Xianglong Zhang Peng Chen Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice Medicine in Microecology Acute liver injury Gut microbiota 16S rRNA |
| title | Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice |
| title_full | Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice |
| title_fullStr | Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice |
| title_full_unstemmed | Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice |
| title_short | Acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice |
| title_sort | acute liver injury progression is associated with dynamic enteric eubiosis alteration in mice |
| topic | Acute liver injury Gut microbiota 16S rRNA |
| url | http://www.sciencedirect.com/science/article/pii/S2590097822000131 |
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