Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato
In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature were at first screened, performing preliminar...
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MDPI AG
2022-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/2/207 |
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| author | Marta Luigi Ariana Manglli Antonio Tiberini Sabrina Bertin Luca Ferretti Anna Taglienti Francesco Faggioli Laura Tomassoli |
| author_facet | Marta Luigi Ariana Manglli Antonio Tiberini Sabrina Bertin Luca Ferretti Anna Taglienti Francesco Faggioli Laura Tomassoli |
| author_sort | Marta Luigi |
| collection | DOAJ |
| description | In 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature were at first screened, performing preliminary studies in accordance with the EPPO standard PM 7/98 (4). Five molecular tests, two conventional RT-PCR and three real-time RT-PCR were found to be suitable and were included in the TPS. Thirty-four laboratories from 18 countries worldwide took part in TPS, receiving a panel of 22 blind samples. The panel consisted of sap belonging to symptomatic or asymptomatic leaves of <i>Solanum lycopersicum</i> and <i>Capsicum annuum</i>. The results returned by each laboratory were analyzed and diagnostic parameters were assessed for each test: reproducibility, repeatability, analytical sensitivity, diagnostic sensitivity and diagnostic specificity. All the evaluated tests resulted in being reliable in detecting ToBRFV and were included in an EPPO Standard PM 7/146—Diagnostics. |
| first_indexed | 2024-03-09T21:16:37Z |
| format | Article |
| id | doaj.art-1d8a58bc0c024bab8157129ea8cbf44e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T21:16:37Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-1d8a58bc0c024bab8157129ea8cbf44e2023-11-23T21:32:01ZengMDPI AGPathogens2076-08172022-02-0111220710.3390/pathogens11020207Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on TomatoMarta Luigi0Ariana Manglli1Antonio Tiberini2Sabrina Bertin3Luca Ferretti4Anna Taglienti5Francesco Faggioli6Laura Tomassoli7Research Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyResearch Centre for Plant Protection and Certification, Council for Agricultural Research and Economics (CREA-DC), Via C.G. Bertero 22, 00156 Rome, ItalyIn 2020, a test performance study (TPS) for the specific detection of tomato brown rugose fruit virus (ToBRFV) was organized in the frame of the H2020 Valitest project. Since no validated tests were available, all the protocols reported in the literature were at first screened, performing preliminary studies in accordance with the EPPO standard PM 7/98 (4). Five molecular tests, two conventional RT-PCR and three real-time RT-PCR were found to be suitable and were included in the TPS. Thirty-four laboratories from 18 countries worldwide took part in TPS, receiving a panel of 22 blind samples. The panel consisted of sap belonging to symptomatic or asymptomatic leaves of <i>Solanum lycopersicum</i> and <i>Capsicum annuum</i>. The results returned by each laboratory were analyzed and diagnostic parameters were assessed for each test: reproducibility, repeatability, analytical sensitivity, diagnostic sensitivity and diagnostic specificity. All the evaluated tests resulted in being reliable in detecting ToBRFV and were included in an EPPO Standard PM 7/146—Diagnostics.https://www.mdpi.com/2076-0817/11/2/207ToBRFVconventional RT-PCRreal-time RT-PCRvalidationperformance criteriaTPS |
| spellingShingle | Marta Luigi Ariana Manglli Antonio Tiberini Sabrina Bertin Luca Ferretti Anna Taglienti Francesco Faggioli Laura Tomassoli Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato Pathogens ToBRFV conventional RT-PCR real-time RT-PCR validation performance criteria TPS |
| title | Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato |
| title_full | Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato |
| title_fullStr | Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato |
| title_full_unstemmed | Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato |
| title_short | Inter-Laboratory Comparison of RT-PCR-Based Methods for the Detection of Tomato Brown Rugose Fruit Virus on Tomato |
| title_sort | inter laboratory comparison of rt pcr based methods for the detection of tomato brown rugose fruit virus on tomato |
| topic | ToBRFV conventional RT-PCR real-time RT-PCR validation performance criteria TPS |
| url | https://www.mdpi.com/2076-0817/11/2/207 |
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